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First substantiated record of the black-striped pipefish, Syngnathus abaster (Actinopterygii: Syngnathiformes: Syngnathidae), in the freshwaters of Bosnia and Herzegovina

100%

Tutman P. , Buric M. , Skaramuca B.

nr 3

Although the ichthyofauna of Bosnia and Herzegovina has been relatively well studied, the knowledge remains incomplete. This report confirms the occurrence of the black-striped pipefish Syngnathus abaster Risso, 1827 in the freshwaters of Bosnia and Herzegovina. A sample specimen of the black-striped pipefish was obtained from the Neretva River in Bosnia and Herzegovina in July 2011. The morphometric measurements and meristic counts were taken. Although the black-striped pipefish has previously been reported to exist in this region, the data were not reliable or conclusive. Therefore the finding reported herewith can be considered the first substantiated record from Bosnia and Herzegovina and extends the previously known distribution range for this species into freshwaters.

Cross-species amplification of microsatellite markers in the invasive spiny-cheek crayfish [Orconectes limosus]: assessment and application

75%

Hulak M. , Kaspar V. , Kozak P. , Buric M. , Filipova L. , Petrusek A.

tom 51

nr 1

73-78

The North American spiny-cheek crayfish, Orconectes limosus (Cambaridae), endangered in its native range, is a widespread invasive species in European waters and conservationally important carrier of crayfish plague. However, its population structure is poorly known, and no informative genetic markers for the species are available. We tested cross-species transfer of microsatellite loci to spiny-cheek crayfish from 5 other crayfish species. Variability of 10 successfully amplifying loci derived from 4 species was then tested in 60 individuals of O. limosus originating from 3 natural populations: the river Danube at Bogyiszló in Hungary, a pond in Starý Klíčov, and the brook Černovický, both in the Czech Republic. The allele number within the populations ranged from 4 to 10 alleles per locus, while heterozygosity levels varied from 0.650 to 0.900 for Ho and from 0.660 to 0.890 for He. No linkage disequilibrium and no null alleles were detected. The selected markers are useful for assessing population structure, intraspecific variation, and paternity studies in spiny-cheek crayfish.