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Isolation of biosynthesis related transcripts of 2,3,5,4'-tetrahydroxy stilbene-2-O-beta-D-glucoside from Fallopia multiflora by suppression subtractive hybridization

100%

Zhao W. , Sheng S. , Liu Z. , Lu D. , Zhu K. , Li X. , Zhao S. , Yao Y.

nr 2

2,3,5,4'-tetrahydroxy stilbene-2-O-ß-D-glucoside (THSG) exerts multiple pharmacodynamic actions, found in Fallopia multiflora, but the biosynthesis pathway of THSG is still unclear. To clear this ambiguity, we constructed suppression subtractive hybridization (SSH) libraries to screen the genes involved in THSG biosynthesis from two F. multiflora varieties, which vary significantly in THSG content. Twelve non-redundant differentially expressed sequence tags were obtained and the full lengths of 4 unreported fragments were amplified by rapid amplification of cDNA ends. We totally got 7 fulllength transcripts, and all of them were aligned to the transcriptome and digital gene expression tag profiling database of four F. multiflora tissues (root, stem and leaf from Deqing F. multiflora and another root from Chongqing F. multiflora; data unpublished) using local BLAST. The results showed that there was a significant, organ specific difference in the expression of fragments and full-length sequences. All the sequences were annotated by aligning to nucleotide and protein databases. Kyoto Encyclopedia of Genes and Genomes pathway analysis indicated that THSG biosynthesis was correlated with multiple life activities.

Mixotrophic denitrification desulfurization wastewater treatment process: bioreactor performance and analysis of the microbial community

100%

Li W. , Lin J. , Zhang L. , Zhu K. , Liu X. , Wang Z. , Cao B. , Guo P.

2016

tom 25

nr 6

The mixotrophic denitrification desulfurization process was adopted in an upflow attached-growth bioreactor for wastewater treatment. The microorganisms in the system were acclimated to remove sulfide, nitrate, nitrite, and organics simultaneously while the contaminants were finally converted to element sulfur, nitrogen gas, and carbon dioxide. The 16S rDNA clone library technique was utilized to investigate the characteristics of the microbial community within this mixotrophic environment. The results showed that Azoarcus was the main sulfur-based denitrification desulfurization bacteria and had a relative abundance of 8.0% in this system. It used sulfide as electron donors, with both nitrate and nitrite as eletron acceptors. Thauera, Vulcanibacillus, and Paracoccus were the main heterotrophic denitrification bacteria and had the relative abundances of 29.5%, 9.82%, and 1.78%, respectively. They utilized arganics as electron donors, with nitrate or nitrite as electron acceptors. The high removal efficiencies of contaminants were attributed to the interaction of these two main species.

First survey of parasitic helminths of goats along the Han River in Hubei Province, China

80%

Yang X. , Gasser R. B. , Fang R. , Zeng J. , Zhu K. , Qi M. , Zhang Z. , Tan L. , Lei W. , Zhou Y. , Zhao J. , Hu M.

nr 3