Wyniki wyszukiwania - Biblioteka Nauki

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Rola hormonów oraz czynników wzrostowych w implantacji zarodka u ssaków

100%

Bukowska D. , Kempisty B. , Ciesiolka S. , Piotrowska H. , Antosik P. , Jaskowski J. M. , Nowicki M.

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tom 70

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nr 03

EN

Implantation is crucial for normal pregnancy and embryo development. Synchronized interaction between the embryo and the endometrium is necessary for the embryo to reach the endometrial site of implantation and for the blastocyst to achieve its normal developmental capacity. The process of implantation is divided into three stages: apposition, adhesion, and invasion. The systems that regulate the course of these mechanisms are based on the secretion of hormones and on the synthesis of selected growth factors. Disruptions in these factor-derived signaling pathways prevent implantation and/or result in abortion, thus reducing fertility. This review presents the molecular basis of embryo implantation. This knowledge may be used in the future to evaluate the ability of potential recipients to receive embryos and to be used for embryo transfer.

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Wybrane aspekty wzrostu i różnicowania się komórek błony śluzowej macicy ssaków w warunkach hodowli pierwotnej in vitro

84%

Wojtanowicz-Markiewicz K. , Kempisty B. , Kranc W. , Dyszkiewicz-Konwinska M. , Budna J. , Ciesiolka S. , Bruska M. , Nowicki M. , Antosik P.

Postępy Biologii Komórki

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2016

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tom 43

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nr 3

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Rola cytokin z rodziny TGF-beta w procesie folikulogenezy, oogenezy oraz dojrzewania oocytów u ssaków

67%

Rybska M. , Kempisty B. , Swierczewska M. , Wozna M. , Ciesiolka S. , Piotrowska H. , Antosik P. , Bukowska D. , Jopek K. , Bruska M. , Nowicki M. , Jaskowski J. M.

Medycyna Weterynaryjna

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2015

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tom 71

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nr 07

EN

The process of oocytes maturation in mammals is regulated by the expression of many types of cell-specific genes. Both nuclear and cytoplasmic maturation is based on activation of biochemical and metabolic pathways that lead to reaching the full maturation stage (MII) of oocytes. The most recent data indicates that the proteins belonging to transforming the growth factor beta (TGF-β) superfamily play an important role in the process of oocytes maturation, as well as of reaching full developmental capacity by these cells. Moreover, it has been clearly showed that the proteins belonging to TGF-β such as inhibins (INHA, INHB) and activins regulate both folliculo- and oogenesis by the stimulation and/or inhibition of several biochemical pathways. In this review, the most recent knowledge about the role of proteins belonging to TGF-β superfamily in the regulation of folliculogenesis, oogenesis and oocytes maturation has been presented and discussed.

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Molekularne aspekty procesu dojrzewania jądrowego i cytoplazmatycznego oocytów u świń

67%

Bukowska D. , Kempisty B. , Ciesiolka S. , Piotrowska H. , Antosik P. , Wozna M. , Porowski S. , Ociepa E. , Maryniak H. , Jaskowski J. M. , Bryja A. , Nowicki M.

Medycyna Weterynaryjna

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2013

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tom 69

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nr 08

EN

The development of pig reproductive biotechnology has made it possible to use this species not only as farm animals but also as important models in biomedical research. Systems based on pig embryos from in vitro fertilization (IVF) are used much more frequently, although the quality of these embryos differs from those produced by in vivo fertilization. The most frequent problems in the IVF procedure are disturbances in both nuclear and cytoplasmic oocyte maturation, and the mechanism of polyspermy specific for this species. Although there has been progress in increasing the number of oocytes produced by in vitro maturation processes, the quality of embryos from these oocytes and their developmental competence is still unsatisfactory. It has been suggested that the most important factor in this process is the establishment of optimal conditions for the development and maturation of oocytes. In this review the authors attempt to explain the molecular basis of oocyte maturation, meiosis resumption, and meiotic spindle assembly. The article is also an opportunity to use the knowledge gained to date in order to increase the efficiency of animal breeding, to evaluate the genetic value of oocytes, as well as to improve assisted reproductive techniques (ART).

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Expression of integrin beta 2 (ITGB2) and zona pellucida glycoproteins (ZP3, ZP3alpha) in developmentally competent and incompetent porcine oocytes

67%

Antosik P. , Kempisty B. , Piotrowska H. , Bukowska D. , Ciesiolka S. , Jeseta M. , Piescikowska I. , Lange K. , Maryniak H. , Jaskowski J. M. , Brussow K. P. , Nowicki M. , Zabel M.

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tom 70

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nr 07

EN

The fertilization potential of mammalian oocytes may be regulated at the molecular level by the expression of species-specific sperm-egg interaction molecules, whose activities and/or cellular distribution determine the recognition and fusion of gemetes. Although there exist studies on the expression of integrins (ITGs) and zona pellucida glycoproteins (ZPs) in developmentally fully competent oocytes, the mRNA levels encoding these proteins in immature and developmentally incompetent porcine oocytes have to be elucidated. Therefore, our aim was to determine the expression of ITGB2, ZP3, and ZP3α mRNAs in porcine oocytes before in vitro maturation (IVM), in oocytes stained with BCB test but colorless, and in BCB positive oocytes after IVM. Porcine cumulus-oocyte complexes (COCs) were collected from 32 crossbred Landrace gilts, and then separated into three groups: (i) oocytes analyzed immediately after collection (n = 50), (ii) oocytes stained with brilliant cresyl blue (BCB+) and remained colorless (BCB–) (n = 50). After collection and/or staining and cultivation, all oocytes were denuded and analyzed regarding ITGB2, ZP3, and ZP3α by QT-PCR. We found a higher expression of ITGB2, ZP3, and ZP3α in oocytes immediately after collection and in BCB+ oocytes compared to BCB– oocytes (P < 0.001, respectively). No differences in the ITGB2 and ZP3 mRNA levels were observed between oocytes after collection and BCB+ oocytes. In addition, BCB– oocytes revealed lower transcript expression of all the genes under study. It is presumed that the similar mRNA levels of ITGB2 and ZP3 in oocytes after collection and in BCB+/ IVM oocytes may be related to (i) a toxic effect of BCB staining and/or to (ii) the degradation of accumulated maternal templates in porcine oocytes. Additionally, lower ITGB2, ZP3, and ZP3α transcript levels point to the down-regulation of the mRNA synthesis of stored maternal transcripts in developmentally incompetent porcine oocytes.

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Kancerogeneza w obrębie błony śluzowej jamy ustnej ssaków w aspekcie badań biomedycznych

67%

Bryja A. , Dyszkiewicz-Konwinska M. , Budna J. , Kranc W. , Chachula A. , Ciesiolka S. , Sumelka E. , Krajecki M. , Bukowska D. , Antosik P. , Nowicki M. , Bruska M. , Kempisty B.

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2017

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tom 73

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nr 02

EN

Neoplastic diseases are a challenge to modern medicine. The understanding of histological, molecular and genetic transformation leading to cancer is essential in establishing methods for earlier diagnosis and better treatment of oral cavity cancer, not only in humans but also in animals – in particular, in dogs and cats, which develop oral cancer spontaneously. There are several methods of cancer modeling that expand research capabilities. The following overview discusses different methods of inducing carcinogenesis in animals and presents animal models used in the study of carcinogenesis. Publications reviewed in the paper discuss markers used in the diagnosis of oral cancer and describe the concept of cancer stem cells.