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EN
The effects of cisplatin and transplatin on the ADP (10 μM) - induced aggregation and secretion in vitro were studied. Platinum compounds had an inhibitory effect on ADP-induced platelet aggregation and secretion of adenine nucleotides and proteins from blood platelets. Cisplatin and transplatin also changed the metabolism of endogenous platelet arachidonate and thromboxne A2 synthesis in platelets stimulated by thrombin.
EN
The effects of cisplatin (cis-diamminedichloroplatinum II, CDDP) and transplatin (transdiamminedichloroplatinum, TDDP) at concentrations o f 0.1, 1 and 10 /¿M on platelet cytoskeleton protein composition were studied in vitro. Cisplatin and transplatin were found to cause the changes of cytoskeleton proteins. On the other hand in the presence of CDDP (20 μM) the percent of G-actin was reduced.
EN
The effects of cisplatin (cisdiamminedichloroplatinum II) at the concentration of 20 µM and transplatin (transdiamminedichloroplatinum II) at the concentrations of 20 µM and 200 and transplatin at the concentration of 200 µM were found to cause a significant increase of thiobarbituric add reactive substances (TBARS). We have demonstrated, that cisplatin - induced platelet lipid peroxidation was enhanced by hypertermia and the presence of plasma had no protective effect on dsplatin or transplatin - stimulated TBARS generation in pig blood platelets. The extracellular GSH (1 mM) protects against dsplatin - induced lipid peroxidation in blood platelets measured as TBARS.
PL
Metodą Maddipati i Marnett (1987) wyizolowano i częściowo scharakteryzowano peroksydazę glutationową z płytek krwi świni. Proces oczyszczania przebiegał kolejno poprzez wysalanie siarczanem amonu, hydrofobową chromatografię na złożu Phenyl-Sepharose CL-4B oraz chromatografię jonowymienną. Elektroforeza w żelu poliakryloamidowym z SDS (w obecności /3-merkaptoetanolu) enzymu otrzymanego w ostatnim etapie oczyszczania ujawniła obecność podjednostki o masie cząsteczkowej 23 kDa. Wykazano, że w płytkach dominującą formą GSH-Px jest selenozależna peroksydaza glutationowa, ktora wykazuje ok. 86% całkowitej aktywności tego enzymu. Selenin sodowy powodował wzrost aktywności otrzymanego enzymu, podczas gdy jony miedzi, NEM i cisplatyna była inhibitorami peroksydazy glutationowej.
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EN
The effects of two doses of UVB radiation (1.08 J/cm2 and 4.32 J/cm2; 1.2 mW/cm2) on blood platelets secretion of adenine nucleotides and proteins and P selectin expression were studied. The effect of UVB radiation on P selectin expression (marker of platelet activation) was compared with the effect of thrombin, which is a strong platelet agonist. The obtained results showed that exposure of blood platelet to UVB radiation caused the release of adenine nucleotides, but proteins in a small amount. Moreover, UVB radiation caused decrease of P selectin expression on blood platelets, in opposite to the effect of thrombin. It seems that UVB radiation can partly activate blood platelets, but the mechanism is needed to be explain.
PL
Badano wpływ promieniowania ultrafioletowego (UV-A, 360 nm) na zachowanie się w płytkach krwi wolnych grup sulfhydrylowych szybko reagujących z DTNB. Wykazano, że poziom grup -SH gwałtownie wzrasta w napromienionych płytkach (15, 30, 60 min, UV-A, 0,5 W/cm 9). Wzrost ten jest tylko w nieznacznym stopniu zależny od grup -SH związanych z materiałem uwalnianym z płytek.
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EN
The purpose of this paper is to briefly review the structure and biological activity of bacterial endotoxin. Endotoxin or lipopolisaccharide (LPS) is the major component of the ou ter membranes of the cell walls of Gramm-negative bacteria. LPS consists of two parts: the hydrophobic lipid A and hydrophylic polysaccharide with contrasting chemical and physical properties. The toxic activity is principally due to its lipid A moiety. LPS is capable of inducing stimulation of immunological system acting on monocytes, macrophages, granulocytes, platelets and endothelial cells leading to the release of inflammatory mediators: eicosanoids, cytokines, chemokines, adhesion molecules, reactive free radicals, platelet activating factor (PAF) and NO'. In the circulation LPS may bind to plasme components such as high-density lipoproteins (HDL) or LPS binding protein (LBP). This complex interacts with a high affinity receptor CD14 expressed on leukocytes, monocytes and macrophages resulting in activation of these cells and inducing pathophysiological effects such as inflammation, septic shock and death. The consequences for the host of all these events are dependent on the extent of LPS exposure and the status of the host defense mechanisms.
EN
The effects of inorganic selenocompounds (sodium selenite and selenate) on the level of reduced glutathione, free sulfhydryl groups of proteins and the activity of S-glutathione transferase (GST) in pig blood platelets were studied. It was shown that selenite, contrary to selenate reduced the amounts of both GSH and -SH of platelet proteins. The concentration of GSH after one-hour treatment of platelets with a high dose of selenite (10-4 M) was reduced by 50%. A decrease in protein thiols was also significant. We observed that the activity of platelet GST was inhibited after incubation of these cells with a toxic dose of selenite but not selenate.
EN
P-selectin is stored in the a granules of blood platelets. Following platelet activation by different agonists, P-selectin is expressed on the cell surface where it mediates adhesion primarily to leucocytes.The aim of the study was to evaluate the direct effect of Proteus mirabilis lipopolysaccharides on the P-selectin expression on the platelet surface measured by cytometric method. The obtained results showed that LPS in vitro induced the expression of P-selectin (CD62) on platelets. It indicates that endotoxin from Gram-negative bacteria can activate blood platelets.
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