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Functionalized nanotubular oxide layer on Ti6Al4V as a regulator and biosensor of bone tissue remodeling

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Kaczmarek-Pawelska A. , Krasicka-Cydzik E.

Archives of Materials Science and Engineering

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2015

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tom Vol. 73, nr 2

53--61

EN

Purpose: Our aim was to obtain functionalized nanotubular oxide layer (TNTs) on Ti6Al4V alloy and evaluate its efficiency as a platform for electrochemical biosensor of bone remodeling markers. It was also crucial to examinate does the amount of bonded bALP and BMP-2 and antibodies depends from nanotubes diameter and their electrochemical properties. Design/methodology/approach: The antibody specific for bALP and BMP-2 were used to functionalize the TNTs on Ti6Al4V. The spectrophotometry and electrochemical measurements (CV and EIS) were used to examinate the functionalization efficiency and comfirmed sensing properties of the functionalized TNTs on Ti6Al4V alloy. Findings: The obtained results confirmed that TNTs can strongly bind antibodies by physioabsorption and may be a proper platform for biosensing of the selected markers. The protein immobilization efficiency depends over the nanotube diameter and their electrical charge. Thermally modified TNTs with 50 nm diameter on Ti6Al4V strongly bind bALP antibodies and bALP and it can be detected amperometrically. BMP-2 quantitatively binds to the functionalized non annealed charged TNTs with 100 nm diameter, and it is possible to detect it using EIS. Research limitations/implications: The biosensors presented in this work are simple and fast, but this construction is a prototype and need to be optimized to be used in bone remodelling diagnostics. Practical implications: Development of the functionalized TNTs on the Ti6Al4V sensitive for physiological concentrations of the bone remodelling markers may be alternative for immunotests in diagnostic of bone diseases. Moreover the TNTs morphology generates nano roughness over the Ti6Al4V surface and functionalized by antibodies strongly bind bALP or BMP-2 and stimulate bone proliferation. Originality/value: Unique value of this research is the statement the amount of bonded markers and antibodies depends from TNTs diameter and electrochemical properties, and that the prototype of novel biosensor electrode was developed.

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Morphological and chemical relationships in nanotubes formed by anodizing of Ti6al4v alloy

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Kaczmarek-Pawelska A. , Krasicka-Cydzik E.

Advances in Materials Science

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2014

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tom Vol. 14, nr 4(42)

12--20

EN

The electrochemical formation of oxide nanotubes on the Ti6Al4V alloy has been so far difficult due to easy dissolution of vanadium reach β-phase of the two phase material. Due to the topographical heterogeneity of the anodic layer in nano and microscale at anodizing of the Ti6Al4V alloy we focused to establish the relationships between nanotube diameters on both phases of the alloy and fluorides concentration in electrolyte. We studied the effect of fluoride concentration (0.5-0.7 wt.%) in 99% ethylene glycol on morphological parameters of nanotube layer on the Ti6Al4V alloy anodized at 20V for 20 min. Nanotubes with diameter ~40-50 nm ±5nm on the entire Ti6Al4V alloy surface in electrolyte containing 0.6% wt. NH4F were obtained. Microscale roughness studies revealed that nanotubular layer on α-phase is thicker than on β-phase. The annealing of nanotube layers at 600°C for 2h in air, nitrogen and argon, typically performed to improve their electrical properties, influenced chemical composition and morphology of nanotubes on the Ti6Al4V alloy. The vanadium oxides (VO2, V2O3, V2O5) were present in surface nanotube layer covering both phases of the alloy and the shape of nanotubes was preserved after annealing in nitrogen.

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Alginate based hydrogel for tissue regeneration: optimization, antibacterial activity and mechanical properties

80%

Kaczmarek-Pawelska A. , Winiarczyk K. , Mazurek J.

Journal of Achievements in Materials and Manufacturing Engineering

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2017

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tom Vol. 81, nr 1

35--40

EN

Purpose: In this work our aim was to reveal the relationship between sodium alginate concentration and crosslinking level, also the ratio of release of the antibacterial additives: silver nanoparticles and metronidazole. Moreover, we examine obtained hydrogel as a potential dressing material for regenerative medicine. Design/methodology/approach: In the research specimens of hydrogels were tested to define their mechanical and physicochemical properties like antibacterial activity against gramnegative Escherichia coli and gram-positive Staphylococcus aureus, viscosity and conductivity. Findings: The concentration of alginate and presence of antibacterial additives influence on the crosslinking level. Mechanical properties of hydrogels are similar to human skin. Only hydrogels with addition of metronidazole and AgNP inhibits bacteria growth after 18 h. In case of gram-negative Escherichia coli both of the aseptic additives inhibits bacteria growth, but sodium alginate hydrogel with silver nanoparticles gives better results in tests with grampositive Staphylococcus aureus . Research limitations/implications: The presence of metronidazole in hydrogel, especially its incorporation and binding with mannuronic and guluronic acid residues must be clarified in more advanced research. Practical implications: Obtained results shows that sodium alginate hydrogels with 0.1mg/ml of alginate, due to its properties are proper as a dressing material. Based on the results, and more advanced tests with metronidazole, we can consider dressing design. Originality/value: Unique value of this work is that we completed the gap in knowledge about the relation of crosslinking level and mechanical properties with are crucial to proper tissue healing and addition of popular aseptic agents.