Wyniki wyszukiwania - Biblioteka Nauki

1

A new case of reciprocal translocation t(10;13)(q16;q21) diagnosed in an AI boar

100%

Danielak-Czech B. , Slota E.

Journal of Applied Genetics

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2007

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tom 48

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nr 4

379-382

EN

A new case of reciprocal translocation t(10;13)(q16;q21) was detected in a hybrid boar (Large White ? Pietrain ? Duroc ? Hampshire) from an artificial insemination (AI) station. Altogether, 258 sires of 4 pure breeds as well as hybrid lines and crossbreeds were investigated. The diagnosis was based on classical cytogenetic examination following the standard protocols of lymphocyte cultures, Giemsa staining and G-, C- and Ag-I banding techniques. The population screening performed was an initial part of a long-term karyotype control system of boars kept at AI stations, which was started by the National Research Institute of Animal Production in Poland in 2007.

2

The utilization of molecular biology methods in farm animals breeding and selection

80%

Slota E. , Rejduch B. , Radko A.

Biotechnologia

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2003

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nr 1

84-92

EN

On the basis of the literature, the new molecular methods useful in animal breeding and selection are described. DNA restriction fragment lenght polymorphism analysis is a tool in the diagnosis of some genetic diseases (RYR1 in pigs, BLAD and DUMPS in cattle). Microsatellite DNA polymorphism is useful in parentage control, genetic characteristic of populations, as well as in gene mapping and marker assisted selection. Cytogenetic analyses are recently supported by fluorescent in situ hybridization (FISH) and primed in situ labelling (PRINS) which make the chromosome aberration diagnosis more precise. One of the expanded method is bio-chip construction for genome analyses.

3

Identification of carriers of the mutation causing coagulation factor XI deficiency in Polish Holstein-Friesian cattle

80%

Gurgul A. , Rubis D. , Slota E.

Journal of Applied Genetics

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2009

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tom 50

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nr 2

149-152

EN

Factor XI (FXI) deficiency is a hereditary coagulation disorder observed in various mammalian species. The molecular basis of coagulopathy has been recognized in Holstein cattle as a 76-bp insertion in the coding region of the FXI gene. Because the disorder seems to have an impact on reproductive traits and udder health in cattle, we tested 103 randomly selected cows, 28 cows with repeat breeding, and 9 cows with recurrent mastitis for the presence of an abnormal FXI allele. Three related cows were diagnosed as carriers.

4

Reproductive performance of a transgenic boar carrying the bovine growth hormone gene (bGH)

80%

Rejduch B. , Slota E. , Sysa P. , Rozycki M.

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nr 3

337-341

EN

The aim of this study was to obtain information on the possible influence of the bovine growth hormone gene (bGH) on gametogenesis and reproductive parameters of a 2-year-old Polish Landrace transgenic boar. The bGH gene construct had been introduced into the zygote of the boar with the use of the microinjection technique. On the basis of the available documentation we established that the fertility of the investigated transgenic (bGH) boar was low in comparison with other animals of the Polish Landrace breed, with a poor libido, ineffective matings and, on average, 3 live piglets less per litter. Samples of testis tissue from the boar were obtained after castration. In total, we observed 100 spermatocytes and all of them had normally paired bivalents. It is possible that the boar?s lower fertility was caused by some, as yet unknown factor.

5

The application of human band specific probe (HSA11p15) to identify a corresponding region in the porcine genome

80%

Rejduch B. , Slota E. , Kozubska-Sobocinska A.

Journal of Applied Genetics

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2004

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tom 45

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nr 3

341-342

EN

The aim of this study was to analyse homology in the telomeric region of chromosome 11 in humans and a corresponding fragment in the porcine genome using the FISH technique. The human band specific probe (HSA 11p15) was used for hybridization with pig chromosomes. The obtained results showed strong signals on human chromosome 11p15, as well as pig chromosome 2p17. Some aspects of the similarity between the human and pig chromosome segments have been discussed.

6

Microdissected bovine X chromosome segment delineates homologous chromosomal regions in sheep and goat

80%

Bugno M. , Zyga A. , Slota E.

Folia Biologica

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2008

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tom 56

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nr 3-4

149-151

EN

In this study we microdissected the proximal part band Xq24 of the bovine X chromosome long arm, in which the Xist gene is located in cattle. The obtained DNA fragment was PCR amplified, labelled and used as a probe for cattle, sheep and goat chromosomes. In cattle, as expected, distinct hybridization signals were observed on Xq24. The painting signals were also observed on Xq24 in sheep and goat. The chromosome painting probes can be used for comparative mapping and searching for internal X chromosome rearrangements in Bovidea and may contribute to the understanding of mammalian sex chromosome evolution.

7

Use of cytochrome b polymorphism for species identification of biological material derived from cattle, sheep, goats, roe deer and red deer

80%

Natonek-Wisniewska M. , Slota E. , Kalisz B.

Folia Biologica

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2010

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tom 58

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nr 1-2

47-50

EN

The objective of this study was species identification of the following biological trace material: skin, blood stains, meat samples and jawbone with a tooth, which were the subject of expert opinion ordered by a court. The expert appraisement was conducted by an analysis of a cytochrome b fragment. The choice of mtDNA fragment for analysis was based on its conservation in mammals which enabled several farm and wild species to be identified with one pair of primers. The PCR product was differentiated by Tsp509I and AluI enzymes. Due to problems with amplification of roe deer DNA, primers specific to this species only, flanking a cytochrome b fragment (Y14951.1), were designed. On the basis of this analysis, it was concluded that the skin sample was derived from a goat, dried blood from a roe deer, the jawbone fromcattle, and twomeat samples froma roe deer and red deer. od from a roe deer, the jawbone fromcattle, and twomeat samples froma roe deer and red deer. This method allowed rapid and efficient identification of several species of mammals using diverse biological material.

8

Evaluation of the effect of centromeric heterochromatin polymorphism on pig fertility

80%

Kozubska-Sobocinska A. , Danielak-Czech B. , Slota E. , Rejduch B.

Journal of Applied Genetics

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1996

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tom 37

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nr 3

293-298

EN

C-banding identified centromeric heterochromatin polymorphism, most often located in a pair no 16, was a basis for selecting animals for the experiment.The aim of the experiment was to assess the impact of centromeric heterochromatin polymorphism on pig fertility, expressed by litter size.The research included the first two litters obtained from 30 pairs of parent with different genotypes as regards the parameter under study.A statistical analysis of the number of offspring from different mating types showed no significant differences between the experimental groups of animals.The results obtaned did not confirm suggested correlation between polymorphism of the centromeric heterochromatin regons and pig fertility, estimated by mean size.Thus the polymorphism cannot be regarded as a selection criterion.

9

Genetic variability among Polish Red, Hereford and Holstein-Friesian cattle raised in Poland based on analysis of microsatellite DNA sequences

80%

Radko A. , Zyga A. , Zabek T. , Slota E.

Journal of Applied Genetics

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2005

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tom 46

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nr 1

89-91

EN

Polymorphism of 11 microsatellite DNA loci was analysed in Polish Red (PR), Hereford and Holstein-Friesian (HF) cattle raised in Poland and genetic distance among these breeds was determined. At the 11 loci (TGLA227, BM2113, TGLA53, ETH10, SPS115, TGLA126, TGLA122, INRA23, ETH3, ETH225 and BM1824) analysed with automated DNA sizing technology, a total of 213 alleles were identified: 76 in PR, 76 in HF, and 61 in Hereford. All the microsatellite DNA markers showed high polymorphism. Polymorphism information content (PIC) calculated for each marker exceeded 0.5, except for the ETH3 locus in Hereford cattle (PIC=0.475), and heterozygosity (H) ranged from 54.1% to as much as 85.2%. The coefficient of genetic distance was 0.354 between PR and Hereford, 0.414 between HF and Hereford, and 0.416 between PR and HF cattle.

10

BSE- identification and prophylactics

80%

Slota E. , Natonek M. , Zyga A. , Rejduch B.

Biotechnologia

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2002

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nr 1

136-141

EN

Prion diseases (for example: scrape of sheep, BSE, CJD of humans) are among the most notable central nervous system degenerative disorders caused by the accumulation of modified cellular protein. The conversion of PrP(C) (the normal cellular protein) into PrP(Sc) (the abnormal disease-causing isoform) involves a conformation change whereby the ?-helical content diminishes and the amount of ? sheet increases. PrP (Sc) is partially resistant to proteases, temperature, high and low pH. Because the incidence of prion diseases is due to several factors, various efforts need to be taken to reduce the scale and consequences of the disease. They include post-mortem and in vivo diagnosis and prophylactics, i.e. monitoring of animals and feed control.

11

Chromosomal aberrations diagnosed in farm animals under cytogenetic control at the National Research Institute of Animal Production

80%

Slota E. , Danielak-Czech B. , Rejduch B. , Kozubska-Sobocinska A.

Biotechnologia

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1999

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nr 4

171-178

EN

Cytogenetic studies, carried out between 1978 - 1998 at the National Research Institute of Animal Production resulted in identification of several chromosomal aberrations as follows: chromosomal chimerism XX/XY in cattle, sheep and horses, Robertsonian translocations and sex chromosome trisomy in cattle, reciprocal translocations in pigs and sheep, pericentric inversion in pigs. The influence of Robertsonian and reciprocal translocations on fertility of aberration carriers was evaluated and economic losses in pigs due to decreased fertility were calculated. In the light of these results it is advisable to continue cytogenetic control of farm animals in Poland, mainly males being used in reproduction.

12

Optimalization of fluorescence in situ hybridization conditions in mare oocytes and mouse embryos

80%

Bugno M. , Jablonska Z. , Slota E.

Folia Biologica

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2009

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tom 57

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nr 1-2

49-55

EN

The aim of the study was to optimize hybridization conditions of molecular probes specific for X sex chromosomes of the domestic horse in mare oocyte chromosomes. Mare oocytes, recovered from slaughterhouse ovaries by scraping the granulosa layer, were cultured in vitro. Metaphase II mature oocytes were treated with hypotonic solution and fixed, followed by hybridization of themolecular probe specific for the X chromosome of the domestic horse. Hybridization of probes specific formouse heterosomes onmouse oocytes and early embryos was performed to verify the FISH technique. Of 438 oocytes analysed, 29% reached metaphase II. Despite many changes in the composition of hypotonic solutions and modification of the FISH protocol, the fluorescence signal was observed in mouse oocytes and embryos but not in mare oocytes.

13

Decrease in pig fertility as a result of reciprocal translocations and associated economic effects on the basis of rcp(7;13)(q13;q46)

80%

Danielak C. B. , Kozubska S. A. , Slota E. , Rejduch B.

Journal of Applied Genetics

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1996

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tom 37

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nr 4

373-384

EN

Reciprocal translocations, very frequently identified in pigs, are the cause of fertitlity decrease. The aim of this work was to provide an objective assessment of the real effect of reciprocal translocation (7;13) on the fertility of carriers and associated economic effects. The experiment has shown that fertility, expressed as a mean litter size, decreased by 48% in comparison to that of the control group. On the basis of simulation account, financial losses incurred a translocation as a result of using carrying boar in a commercial herd were estimated at about 8,000 USD for natural mating and at about 162,000 USD for artificial insemination. The results obtained show the need for introducing a system for cytogenetic control of boars producing small litters into the Polish swine improvement programme.

14

Factors responsible for modulation of ribosomal RNA transcription

80%

Wnuk M. , Slota E. , Kotylak Z. , Bugno M.

Folia Biologica

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2006

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tom 54

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nr 1-2

1-8

EN

The aetiology of variation in transcription of ribosomal genes is still an open question. Investigations carried out in many organisms showed that the variation depends not only on genetic mechanisms such as rDNA methylation, elimination of rDNA or the position effects. In this review, we discuss the results of the studies on repression of rRNA transcriptional activation from protein factors to conditions of cell culture, which may influence the variation of nucleolar organizer region activity.

15

Cytognetic analysis of beef cattle

80%

Rejduch B. , Slota E. , Switonski M.

Genetica Polonica

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1994

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tom 35

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nr 4

323-332

EN

A karyotpe analysis of 565 pedigree beef cattle was carried out as part of cytogenetic studies conducted at the Department of Immuno- and Cytogenetics of the National Research Institute of Animal Production.Using CBG-, GTG- and RBA- binding techniques, 16 cases of the 1;29 Robertsonian translocation were diagnosed in the Charolais breed (two bulls) and hybrids from the Blonde d' Aquitane bulls (4 bulls and 10 heifers).A homozygous form of translocation was identifiedin one heifer.A testicular biopsy and meiotic chromosome analysis were carried out in a bull-carrier of the 1;29 translocation .The presence of a trivalent, characteristic of a centric fusion, was observed.The population under study also showed XX/XY leucocytic chimerism in two young bulls.

16

Detection of the XXY trisomy in a bull by using sex chromosome painting probes

71%

Slota E. , Kozubska-Sobocinska A. , Koscielny M. , Danielak-Czech B. , Rejduch B.

Journal of Applied Genetics

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2003

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tom 44

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nr 3

379-382

EN

Two cattle chromosome painting probes, identifying X and Y heterosomes, were applied to verify the diagnosis of XXY trisomy in an 8-month-old bull of the Polish Red breed. The probes were obtained after chromosome microdissection and labelled with biotin-16-dUTP. In all metaphase spreads, three fluorescence signals were observed - two X and one Y - confirming the diagnosis of a pure XXY trisomy.

17

Genetic variation of Polish endangered Bilgoraj horses and two common horse breeds in microsatellite loci

71%

Zabek T. , Nogaj A. , Radko A. , Nogaj J. , Slota E.

Journal of Applied Genetics

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2005

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tom 46

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nr 3

295-305

EN

Genetic variation of endangered Bi?goraj horses and two common Polish horse breeds was compared with the use of 12 microsatellite loci (AHT4, AHT5, ASB2, HMS2, HMS3, HMS6, HMS7, HTG4, HTG6, HTG7, HTG10, VHL20). Lower allelic diversity was detected in all investigated populations in comparison to other studies. Large differences in the frequencies of microsatellite alleles between Bi?goraj horses and two other horse breeds were discovered. In all polymorphic loci all investigated breeds were in the Hardy-Weinberg equilibrium. Mean Fis values and the results of a test for the presence of a recent bottleneck were non-significant in all studied populations. Comparable values of observed and expected gene diversity indicate no substantial loss of genetic variation in the Bi?goraj population and two other breeds. The lowest variability observed in the investigated group of Thoroughbred horses was confirmed. About 10% of genetic variation are explained by differences between breeds. Values of pairwise Fst and two measures of genetic distance demonstrated that Bi?goraj horses are distantly related to both common horse breeds.

18

Chromosomal homology between the human and the bovine DMRT1 genes

61%

Bratus A. , Bugno M. , Klukowska-Rotzler J. , Sawinska M. , Eggen A. , Slota E.

Folia Biologica

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2009

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tom 57

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nr 1-2

29-32

EN

Doublesex and mab-3 related transcription factor 1 (DMRT1) is considered to be the most conserved gene among loci involved in the molecular pathways of animal sexual development. In the majority of the extensively examined vertebrates, its function is limited to the upstream or downstream testis regulators acting during embryogenesis. Our present study demonstrated the structural homology between DMRT1 orthologos in human and cattle. A BAC clone with a specific bovine sequence of the gene was used in the FISH mapping experiments. The physical localization of DMRT1 in cattle (BTA 8q17) was determined and its homology to the human locus was shown (HSA 9p24.3). Furthermore, another BAC probe, containing the sequence of the human homologue (pBACe3.6), generated hybridisation signals on bovine metaphase chromosomes and indicated the physical location of the autosomal bovine DMRT1 locus. Further investigations of the gene in domestic animals might provide more support for its conservative status and may help in understanding the molecular mechanisms involved in the occurrence of sexual abnormalities often diagnosed in livestock.

19

Additional chromosome in a child as a result of a balanced reciprocal translocation t(12;18)(p13;q12) in his mother's karyotype

61%

Lassota M. , Przelozna B. , Plodzien M. , Bugno M. , Wnuk M. , Kotylak Z. , Slota E.

Journal of Applied Genetics

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2005

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tom 46

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nr 4

419-421

EN

In this case report we present a child with an additional chromosome in the karyotype. The karyotypes of the boy and his parents were analyzed by use of a conventional banding technique (GTG) and fluorescence in situ hybridization (FISH). Probes painting whole chromosomes 12 and 18 were used in FISH. Cytogenetic examination of the parents revealed that his mother was carrying balanced reciprocal translocation between chromosomes 12 and 18. Her karyotype was described as 46,XX,t(12;18)(p13;q12). Father's karyotype was normal, described as 46,XY. The boy's karyotype was defined as 47,XY,+der(18)t(12;18)(p13;q12). The additional chromosome appeared probably due to 3:1 meiotic disjunction of the maternal balanced translocation, known as tertiary trisomy. The mother displayed a normal phenotype and delivered earlier a healthy child. However, the boy with the unbalanced karyotype shows multiple congenital abnormalities.