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EN
In this study, when acrylamide is taken into the body by nutrition, it was aimed to investigate its eco- toxic and/or carcinogenic effects on the liver. The Azaserine-Rat model developed by Longnecker and Curphey was used in this research, and in total 60 Wistar Albino race male rats were used, including 10 rats in each group. The rats were fed for 16 weeks by adding acrylamide at rates of 5 mg/kg/day and 10 mg/kg/day in drinking water. Moreover, neoplastic structures were formed by azaserine application and its effect on the development of these neoplastic structures was also investigated. As a result of this study, it was determined that ecotoxic and histopathological alterations, together with atypical cells, focuses formed in the livers of the rats in the group to which azaserine was applied and in the livers of the rats in the groups that included 5 and 10 mg/kg/day acrylamide in their drinking water. Moreover, it was found that the development (average focus diameter and focus volume) of neoplastic structures formed with azaserine was increased by 5 and 10 mg/kg/day acrylamide. These results make it possible for the probability of acrylamide to be a cancer initiator in the livers of rats.
EN
The aim of the study was to evaluate oxidative stress in cows undergoing normal parturition and cows suffering from dystocia. The erythrocytic glutathione peroxidase (GSH-Px) activity, plasma vitamin A and ß-carotene concentrations, and paraoxonase - PONI activity were lowered (P0.001, P<0.01, 0.05, and 0.05, respectively) in cows with dystocia compared to normal calving cows. The erythrocytic malondialdehyde (MDA) concentration was markedly increased in the dystocia group compared to normal calving group. However, erythrocytic glutathione (GSH), catalase (CAT), and superoxide dismutase (SOD) activites, and plasma vitamin E and MDA concentrations were not significantly changed in the dystocia-affected cows in comparison to eutocia cows. In the difficult calving cows, there were significant correlations between the activities of GSH-Px and SOD (r=-0.4l, P<0.01), plasma ß-carotene levels and paraoxonase activity (r=0.34, P<0.05), body condition score (BCS) and plasma MDA (r=0.46, P<0.05). Similarly in the eutocia cows there were significant correlations between CAT activity and MDA concentration (r=-0.76, P<0.01), levels of plasma ß-carotene and PONI (r=0.58, P<0.01), BCS and MDA concentrations (r=0.50, P<0.05), and BCS and vitamin E (r=0.53, P<0.05) concentrations. These results suggests that evaluation of plasma vitamin A and ß-carotene concentrations, PONI and GSH-Px activities, and MDA concentration seems to be useful in the assessment of dystocia in cows.
EN
The study aimed to define the anatomical and immunohistochemical characteristics of the vomeronasal organ in six male dogs. The organ was a bilateral structure located in the foremost ventral part of the nasal cavity. It was tubular in shape, and 25.36 ± 1.89 mm in length. While the caudal end of the organ was blind, the cranial one consisted of a bilateral vomeronasal duct. At the histological examination, two types of epithelium were seen: sensory and non-sensory. The vomeronasal organ was largely surrounded by "J shaped" vomeronasal hyaline cartilage. The perichondrium of the vomeronasal hyaline cartilage consisted of chondroblasts. At the higher magnification, the strong immune positive reaction with anti-oestrogen antibody was observed only in the sensory epithelium of the organ.
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