Wyniki wyszukiwania - Biblioteka Nauki

1

Strategia antysensowych oligonukleotydow

100%

Stec W. J.

Biotechnologia

|

1994

|

nr 4

5-15

2

Tendencje rozwojowe światowej biotechnologii

100%

Stec W. J.

Biotechnologia

|

2000

|

nr 1

3

Dlaczego nalezy przyjmowac nowoczesne biotechnologie?

100%

Stec W. J.

Postępy Biologii Komórki

|

1994

|

tom 21

|

nr 2

105-120

4

Na przykladzie biologii molekularnej, biochemii i chemii

88%

Stec W. J.

Nauka i Przyszłość

|

1996

|

tom 06

|

nr 12

1

5

Oligonukleotydy: czy tylko fragmenty kwasow nukleinowych?

63%

Stec W. J. , Koziolkiewicz M.

Kosmos

|

2000

|

tom 49

|

nr 3

489-501

6

Perspectives for medicinal applications of synthetic oligonucleotides: antisense technology

63%

Stec W. J. , Guga P.

Biotechnologia

|

1996

|

nr 4

7

Crystals of recombinant human tumor necrosis factor

45%

Joachimiak A. , Giel M. , Klysik J. E. , Stec W. J. , Barciszewski J.

Bulletin of the Polish Academy of Sciences. Biological Sciences

|

1991

|

tom 39

|

nr 1

8

Biotechnology in Poland

44%

Stec W. J.

Biotechnologia

|

1993

|

nr 4

7-17

9

A mutational study of individual functional groups on the G6 base of the 10-23 deoxyribozyme core

38%

Nawrot B. , Widera K. , Wojcik M. , Rebowska B. , Goss W. , Mikolajczyk B. , Stec W. J.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr Supplement 1

10

Mapping of the function phosphate groups in the catalytic core of DNAzyme 10-23

38%

Nawrot B. , Winder K. , Wojcik M. , Rebowska B. , Goss W. , Stec W. J.

Acta Biochimica Polonica

|

2006

|

tom 53

|

nr Supplement 1

11

Synthesis and interaction with thymidylate synthase of 5'-dithiophosphate and 5'-fluorothiophosphate of thymidine

38%

Golos B. , Misiura K. , Olesiak M. , Okruszek A. , Stec W. J. , Rode W.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 1

EN

Thymidine-5'-fluorothiophosphate, dTMP(S)-F, was synthesized by the oxathiaphospholane, and thymidine 5'-dithiophosphate, dTMPS2, by the dithiaphospholane, method. To estimate the role of 5'-phosphate group ionization in binding of pyrimidine nucleotides by thymidylate synthase, dTMP(S)-F was studied as an inhibitor of mouse tumour (L1210) enzyme, and its inhibitory properties were compared with those of dTMPS2, a close dTMP analogue. While dTMPS2 proved to be an inhibitor, competitive vs dUMP, with Kiapp = 94 μM, the 5'-fluorothiophosphate congener displayed no activity, indicating that the enzyme requires for binding the presence of a dianionic 5'-phosphate group in a nucleotide.

12

Antisense oligonucleotides to PAI-1 mRNA as potential agents for therapeutic intervention in cardiovascular diseases

32%

Cierniewski C. S. , Pawlowska Z. , Pluskota E. , Stasiak M. , Kobylanska A. , Misiura K. , Maciaszek A. , Koziolkiewicz M. , Stec W. J.

Biotechnologia

|

1996

|

nr 4

13

Synthesis, biochemical and biological studies on oligonucleotides bearing a lipophilic dimethoxytrityl group

32%

Misiura K. , Wojcik M. , Krakowiak A. , Koziolkiewicz M. , Pawlowska Z. , Pluskota E. , Cierniewski C. S. , Stec W. J.

Acta Biochimica Polonica

|

1998

|

tom 45

|

nr 1

EN

Dimethoxytritylphosphono-oligonucleotide conjugates have been prepared. They are totally resistant to nucleases present in human serum and do not affect cleavage of a complementary oligoribonucleotide by RNase H. Conjugates possessing a phosphate backbone gave better antisense inhibition of expression of plasminogen activator inhibitor type-1 within endothelial cells as compared with unconjugated oligonucleotides.

14

The epidermal growth factor-like domain from tissue plasminogen activator. Cloning in E.coli, purification and ESR studies of its interaction with human blood platelets

32%

Pietrucha T. , Stec W. J. , Okruszek A. , Uznanski B. , Koziolkiewicz M. , Wilk A. , Plucienniczak A. , Swiatkowska M. , Cierniewski C. S.

Acta Biochimica Polonica

|

1994

|

tom 41

|

nr 1

EN

To examine whether the epidermal growth factor (EGF)-like domain Pr047-Asps7 is involved in the interaction of tissue plasminogen activator (t-PA) with platelets, we have expressed this domain in £. coli. The peptide fragment was produced from a plasmid expression vector as a fusion protein with P*galactosidase Meti-Valm at high yield in eight clones of E. coli. The fusion protein was purified and subjected to mild acid hydrolysis with formic acid, then the peptide Pro47-Asps7, identified by immunoblotting using specific antibodies to t-PA, was isolated by HPLC. After incubation with blood platelets spin labelled with 16-doxylstearic acid or 5-doxylstearic acid, the Pro47-Asps7 peptide fragment reduced fluidity of the membrane lipid bilayer to the same extent as did intact t-PA as indicated by ESR measurements. Our data suggest that the EGF-like domain of t-PA can directly interact with blood platelets and thus it seems to contain those sites of the t-PA molecule that bind the platelet membrane components.

15

Co dalej z polska biochemia i biotechnologia?

32%

Schramm R. W. , Stec W. J. , Fikus M.

Biotechnologia

|

1992

|

nr 1

5-12

16

Synthesis and expression in E. coli of the gene for human tumor necrosis factor (h alpha TNF)

26%

Klysik J. , Konarzewska-Zglinska A. , Galazka G. , Uznanski B. , Okruszek A. , Guga P. , Wilk A. , Koziolkiewicz M. , Tchorzewski H. , Zembala M. , Stec W. J.

Archivum Immunologiae et Therapiae Experimentalis

|

1991

|

tom 39

|

nr 4

17

Inhibition of plasminogen activator inhibitor release in endothelial cell cultures by antisense oligodeoxyribonucleotides with a 5' -end lipophilic modification

26%

Kobylanska A. , Pluskota E. , Swiatkowska M. , Wojcik M. , Cierniewska-Cieslak A. , Krakowiak A. , Boczkowska M. , Pawlowska Z. , Okruszek A. , Koziolkiewicz M. , Cierniewski C. S. , Stec W. J.

Acta Biochimica Polonica

|

1999

|

tom 46

|

nr 3

EN

A series of conjugates containing residues of lipophilic alcohols covalently bound to 5'-end of oligodeoxyribonucleotides targeted against human plasminogen activator inhibitor (PAI-1) mRNA was synthesized via the oxathiaphospholane approach. The highest anti-PAI-1 activity in EA.hy 926 endothelial cell cultures was found for conjugates containing menthyl or heptadecanyl groups linked with an oligonucleotide complementary to a segment of human PAI-1 mRNA. The phosphodiester antisense oligonucleotides, which otherwise exhibit only limited anti-PAI-1 activity, were found to be more active than phosphorothioate oligonucleotides when conjugated to lipophilic alcohol residues. For menthyl conjugates an evidence of antisense mechanism of inhibition was found.