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1

Cercarial dimensions and surface structures as a tool for species determination of Trichobilharzia spp.

100%

Podhorsky M. , Huzova Z. , Mikes L. , Horak P.

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nr 1

28-36

EN

Cercariae of bird schistosomes are traditionally considered to be very similar in their morphological characteristics. In order to solve the problem, we tested some methods which might be suitable for cercarial differentiation. Fourteen isolates of three Trichobilharzia species (T. szidati, T. franki, T. regenti) occurring sympatrically in Central Europe were used. Dimensions of individual cercariae do not represent a useful criterion for identification, because the intraspecific variability exceeds the interspecific one. On the other hand, chaetotaxy appears a promising way for discrimination, although some sensory papillae do not stain sufficiently with silver nitrate. The papillary pattern (i.e. number and relative position of papillae) is specific for all Trichobilharzia species studied by us. Therefore, we compiled an identification key for the three Trichobilharzia species. In addition, we tried to find species-specific surface saccharide epitopes; none of the labeled lectin probes can be used as a speciesspecific marker.

2

Analysis of surface carbohydrates of Eudiplozoon nipponicum [Monogenea, Diplozoidae] using lectin binding techniques

100%

Hricova I. , Horak P. , Gelnar M. , Koubkova B.

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tom 44

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nr 3

3

The retinal fascin gene 2 [FSCN2] - partial structural analysis and polymorphism detection in dogs with progressive retinal atrophy [PRA]

100%

Horak P. , Knoll A. , Dvorak J.

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nr 4

EN

The retinal fascin 2 gene (FSCN2) underwent a molecular analysis, a search for polymorphisms and an evaluation as a candidate gene for retinopathies in dogs. Specific fragments of the gene encompassing partial exon 1 and intron 1, and exons 2-5 with respective introns were sequenced and these data were deposited in the GenBank database. Three distinct polymorphic sites detectable with PCR-RFLP were found - AM050719: g.237G > A, AM050719: g.525A > G, and AM050720: g. 1071A > G. No positive associations between these polymorphisms and the PRA-clinical status were observed in the investigated population consisting of Poodles, American Cocker Spaniels, and English Cocker Spaniels. In spite of that, the FSCN2 gene remains an excellent candidate gene for retinopathies in dogs and the results can contribute to further research in this field.

4

Radix spp.: Identification of trematode intermediate hosts in the Czech Republic

75%

Hunova K. , Kasny M. , Hampl V. , Leontovyc R. , Kubena A. , Mikes L. , Horak P.

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tom 57

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nr 3

EN

Lymnaeid snails of the genus Radix serve as intermediate hosts of some schistosomes and fasciolids. In Europe, delineation of species within the genus Radix is unresolved and, therefore, spectrum of snail hosts susceptible to trematode infections is under discussion. We used and compared three criteria for species delineation using snails collected at 43 localities. (a) Sequence analysis of ITS-2 rDNA disclosed that the collected snails belong to four species — R. auricularia (Linnaeus, 1758), R. peregra (Müller, 1774), R. lagotis (Schrank, 1803) and R. labiata (Rossmaessler, 1835) (criteria and names are based on the work of Bargues et al. 2001). Occurrence of R. peregra in the Czech Republic was confirmed by molecular data for the first time. (b) Characterization of reproductive system disclosed differences in location, size and shape of bursa copulatrix and its ductus. Unfortunately, some R. labiata specimens shared morphological features of reproductive organs with R. lagotis. (c) Statistical analysis of shell morphology proved that significant differences exist among particular species. One prediction model showed that correct classification of species may be achieved in 82-84% of cases. However, identification of individual snails in the field (without knowledge of respective snail population and use of statistical tools) still remains a complicated issue due to overlaps of shell characteristics. Concerning the role in trematode transmission, R. lagotis, R. labiata and R. peregra are susceptible to Trichobilharzia regenti. Also, successful experimental infections of R. lagotis and R. labiata by Fascioloides magna were accomplished.

5

Modulatory effect of the Euro-Lupus low-dose intravenous cyclophosphamide regimen on circulating immune cells in systemic lupus erythematosus

63%

Gabcova G. , Horak P. , Mikulkova Z. , Skacelova M. , Zehnalova S. , Smrzova A. , Petrackova A. , Mrazek F. , Kriegova E.

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nr 6