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1

Quality and composition of milk with different somatic cell count of two breeds of dairy goats

100%

Laurinaviciute V. , Siugzdaite J. , Urbsiene D.

Medycyna Weterynaryjna

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2004

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tom 60

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nr 11

1168-1170

2

Laboratory diagnosis of mycoplasma infection in young cattle

100%

Gabinaitiene A. , Siugzdaite J. , Zilinskas H.

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nr 1

EN

The aim of this study was to detect Mycoplasma species in the respiratory tract of 110, 310 and 510 day-old groups of cattle by serological, bacteriological and histopathological investigations. Antibodies against M. bovis were found in 75% of the 110 day-old, in 50% – of the 310 day-old and in 55% – of the 510 day-old groups of cattle. Bacteriological examination of the samples from nasal cavities revealed that Mycoplasma carriers were found in 60% of the 110 day-old group of cattle, 40% of the 310 day-old and 40% of the 510 day-old group of cattle. Using the PCR method Mycoplasma was isolated from 25% of lung samples of the 510 day-old group of cattle. Mycoplasma bovis and Mycoplasma dispar were confirmed by serological investigations. Foci of bronchointerstitial pneumonia were determined by histopathological examination in 27.5% of lung samples. Mycoplasma bovis was isolated in 72.7% of bronchointerstitial pneumonia cases. Data processing with an SPSS 13.0 statistical package led to the conclusion that Mycoplasma bovis was found more frequently in the 110 day-old group of cattle (the youngest age group in this study) rather than in the 310 and 510 day-old groups of cattle (χ2 = 6.531; p = 0.038). The results obtained led to the conclusion that serological, bacteriological and histopathological examinations are important in detecting particular animal – carriers of Mycoplasma.

3

Assessment of milking systems including microbiological and technological aspects

100%

Miseikiene R. , Siugzdaite J. , Gaworski M.

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nr 46 Agric.Eng.

53-59

4

Active immunization against enzootic pneumonia in pig farm with poor housing and environmental conditions

100%

Siugzdaite J. , Garlaite K. , Siugzda R.

Medycyna Weterynaryjna

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2005

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tom 61

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nr 01

EN

The objective of the present study was to determine the influence of inactivate vaccine against Mycoplasma hyopneumoniae (M. hyopneumoniae) in a pig farm with poor housing and environmental conditions. The vaccine efficacy investigation was carried out at a medium-sized pig farm. A total of 740 piglets were selected for the experiment. The piglets were divided into 2 equal groups: vaccinated and non-vaccinated. Forty-four piglets were allocated in two groups of twenty-two piglets each. The first group of twenty-two piglets was vaccinated and 14 days later it was revaccinated. The control group of twenty-two non-vaccinated piglets was injected by a placebo. Blood samples with respect to antibody formation against M .hyopneumoniae were taken at 7, 21, 35, 49, 63, 91, 119 and 147 days-of-age and investigated by ELISA. Piglet's life weights were measured individually at the age of 7, 51, 81 and 171 days during their transfer to another unit. Back fat thickness, muscle thickness and lean meat content were measured before slaughter. Pulmonary lesions caused by M. hyopneumoniae were scored at slaughter by the Goodwin method. Lungs with gross lesions were investigated microbiologically. The ELISA tests of serum showed that antibodies against M. hyopneumoniae in the vaccinated group formed after 14 days of the second vaccination and remained till the 147'h day. [n the non-vaccinated group seroconversion started on the 91't day. Dai|y weight gain (DWG) showed an increase of 33 grams (g) in vaccinated pigs compared with non-vaccinated ones. The percentage of lean meat was significantly, greater (difference 3.26%) in the vaccinated group. Greater proportion of lung surface with pneumonic lesions was detected in non-vaccinated pigs using all methods. Eight lung samples with lesions typical of M. hyopneumoniae from the non-vaccinated group were investigated microbiological|y and M. hyopneumoniae was cultured from all specimens (100%). The study demonstrates that pigs vaccinated against M. hyopneumoniae with inactivate bacteria under poor housing and environmental conditions achieved higher DWG, lean meat percentage and lower lung lesions than the pigs from the control group.

5

Antimicrobial susceptibility of Staphylococcus spp. isolated from dogs with pyoderma

88%

Siugzdaite J. , Zamokas G. , Grigonis A. , Macijauskas V. , Lasys V.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 08

EN

The aim of this study was to evaluate antimicrobic resistance in canine staphylococci to the major classes of antimicrobials used in veterinary dermatology. The data was collected in 2002-2006 in the Dr L. Kriauceliunas small animal clinic of the Lithuanian Veterinary Academy. Clinical evaluation of 163 dogs was performed together with microscopical, mycological and bacteriological examinations. The clinical evaluation included the age of the dog, breed, sex, hair structure and season of the year. The authors found out that during the 5 year period males got the disease more often than females (52.98% versus 42.02%) and dogs at the ages of 1-5 years were the most common patients (43.9%). Bacterial skin diseases were more common in short-haired dogs (61.81%, p < 0.05). Long-haired dogs were more likely to be affected by bacterial skin diseases in the cold season (p < 0.05). Bacteriological examination revealed that the most common cause of bacterial skin infections was Staphylococcus intermedius (71.76%, p < 0.05) and Staphylococcus aureus (23.68%, p < 0.05). Mixed cultures (Staphylococcus spp., Streptococcus spp., Pseudomonas spp. and Escherichia coli) were found to be the cause of 4.56% (p < 0.05) of all bacterial skin diseases. S. intermedius and S. aureus strains exhibited the best sensitivity to ciprofloxacin, amikacin, methicillin (100%) and to cephadroxil (respectively 91.75% and 100%). S. intermedius strains were most resistant to sulphanilamides/trimethoprim (82.28%), S. aureus - to amoxicillin (86.0%).

6

Influence of teats disinfection before milking on the bacterial contamination of the skin of teats and bulk tank milk

88%

Siugzdaite J. , Miseikiene R. , Stankevicius H. , Tacas J. , Tusas S.

Medycyna Weterynaryjna

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2005

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tom 61

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nr 02

7

Influence of puerperal metritis on the first ovulation after calving in dairy cows

75%

Juodzentis V. , Siugzdaite J. , Palubinskas G. , Juodzente D. , Rekesiute A. , Zilinskas H.

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tom 23

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nr 4