Plant derived extracts and phytochemicals have long been a subject of research in an effort to develop alternatives to conventional insecticides but with reduced health and environmental impact. In the present study, the aphid settling deterrent activity of extracts obtained from garlic (Allium sativum L.), wormwood (Artemisia absinthium L.) and tansy (Tanaceum vulgare L.) against peach potato aphid (Myzus persicae [Sulz.]) was investigated. The settling of M. persicae on host plant leaves was strongly deterred by soap 1%, soap 4%, Bioczos Forte 2% + soap 1%, Bioczos 1% + tansy extract 1%. Their effect was observed after 1 hour aphid had got access to the plants and it lasted until the end of the experiment, i.e. 24 hours after application. After 24 hours, aphid settling was deterred by the following combinations of extracts: Bioczos 2%, Bioczos 2% + soap 1%, Bioczos 5%, Bioczos 5% + soap 1%, Bioczos 1% + wormwood extract 1%, Bioczos 1% + wormwood extract 1% + soap, tansy extract 1% + wormwood extract 1% and tansy extract 1% + wormwood extract 1% + soap 1%.
The large white butterfly Pieris brassicae (L.) (Lepidoptera: Pieridae) is a cosmopolitan insect that is specialized to feed on the plant family Brassicaceae. The caterpillars may cause severe losses in yield of cabbage plants. In search of environmentally safe control chemical substances, the effect of 17 natural monoterpenes on the feeding activity and food assimilation of P. brassicae larvae was studied. According to the analysis of behavioural and physiological effects caused by the substances applied in the present study, these chemicals can be divided into into five groups: highly active deterrents that practically completely inhibited the feeding of caterpillars (α-phellandrene and β-ionone), strong deterrents (α-terpinene and α-ionone), relatively strong deterrents (citronellol, (-)-linalool, p-cymene), moderate deterrents ((+)-fenchone, (+)-R-limonene, γ-terpinene, and (S)-(+)-carvone), and inactive substances (α-pinene, eucalyptol, bornyl acetate, geraniol, thymol, and L-menthol). α-Phellandrene, β-ionone, α-ionone, α-terpinene, citronellol, and (-)-linalool may be classified as preingestive deterrents that act before food ingestion. p-Cymene may be considered as postingestive deterrent that causes a loss in body weight.
PL
Bielinek kapustnik Pieris brassicae (L.) (Lepidoptera: Pieridae) jest kosmopolitycznym gatunkiem wyspecjalizowanym w żerowaniu na roślinach z rodziny Brassicaceae. Gąsienice mogą powodować poważne straty u roślin kapustowatych. W poszukiwaniu przyjaznych środowisku środków ochrony roślin zbadano wpływ 17 naturalnych monoterpenoidów na żerowanie i przyrost masy ciała gąsienic P. brassicae. Analiza behawioralnych i fizjologicznych efektów wywołanych przez poszczególne substancje pozwoliła na zaliczenie ich do pięciu grup: bardzo aktywne deterenty, które prawie całkowicie zniechęcały gąsienice do żerowania (α-felandren i β-jonon), silne deterenty (α-terpinen i α-jonon), stosunkowo silne deterenty (citronellol, (-)-linalool, p-cymen), słabe deterenty ((+)-fenchon, (+)-R-limonen, γ-terpinen i (S)-(+)-karwon) oraz substancje nieaktywne (α-pinen, eukaliptol, octan bornylu, geraniol, tymol i L-mentol). Ponadto najbardziej aktywne deterenty (α-felandren, β-jonon, α-jonon, α-terpinen, citronellol, (-)-linalool) można określić jako deterenty smakowe działające przed pobraniem pokarmu, zaś p-cymen – jako deterent metaboliczny działający po pobraniu pokarmu i powodujący spadek masy ciała gąsienic.
The consequences of pearling and cutting (barley Hordeum vulgare L.), and roasting and cutting (buckwheat Fagopyrum esculentum Möench) on the development and food consumption of the confused flour beetle Tribolium confusum Duv. were studied. The factors affecting the increase in the T. confusum population, and food consumption effectiveness (the proportion of wasted food in the whole amount of the used product) were different in barley and buckwheat. The best barley product for T. confusum was the flour. The population number, the proportion of imagines, and the effectiveness of food consumption were relatively high for cut groats, compared to whole barley. The toughness of the whole groats was most likely the cause of the lowest suitability of this product for the confused flour beetle. Neither the size of the cut groats nor the pearling of barley had any effect on T. confusum development and food consumption effectiveness. The best buckwheat product for T. confusum was the whole hulled non-processed groats. In these groats, the total population number, the proportion of imagines, and the effectiveness of food consumption were relatively high compared to cut groats. The total population number was the lowest in the steamed groats. The decrease in nutrients and B vitamins due to the removal of the embryo and aleurone layer during the breaking process of buckwheat, was possibly the main factor affecting T. confusum development and food consumption in buckwheat.
The confused flour beetle is one of the most destructive pests of stored products. The food spectrum of this species is very broad. In the experiments, an attempt to evaluate the susceptibility of selected gluten free products to feeding by Tribolium confusum was made. The best food for the confused flour beetle was buckwheat flour, on which the beetle showed the most rapid development. On buckwheat flour, the beetle produced the highest number of 2nd generation beetles and pupae as well as the lowest number of larvae in the experimental time period. The species can also be a threat to multigrain flour concentrate and bread dough, although the development of T. confusum was slightly slower on these products, and the number of larvae was the highest. Rice flour was the least susceptible to T. confusum infestation.
Aphid feeding induces various defense signaling mechanisms in plants. The recognition of feeding activities by plants occurs through the use of transmembrane pattern recognition receptors (PRRS) or, acting largely inside the cell, polymorphic nucleotide-binding leucine-rich-repeat (NB-LRR) protein products, encoded by most R genes. Activation may induce defensive reactions which are the result of highly coordinated sequential changes at the cellular level comprising, among other changes, the synthesis of signaling molecules. The ensuing plant responses are followed by the transmission of defense response signal cascades. Signals are mediated by bioactive endogenous molecules, i.e. phytohormones, such as jasmonic acid (JA), salicylic acid (SA), ethylene (ET), abscisic acid (ABA), gibberellic acid (GA) and free radicals such as hydrogen peroxide (H₂O₂) and nitric oxide (NO) which independently provide direct chemical resistance. Plant-induced defenses are also regulated by a network of inter-connecting signaling pathways, in which JA, SA, and ET play dominant roles. Both synergistic and inhibitory aspects of the cross-talk among these pathways have been reported. This paper presents molecular mechanisms of plant response to aphid feeding, the precise activation of various endogenous bioactive molecules signaling in the response of many plant species and their participation in the regulation of numerous defense genes, which lead to a specific metabolic effect. Selected important points in signal transduction pathways were also discussed in studies on plant response to aphid feeding.
The aim of this work was to investigate the relative susceptibility of selected varieties of yellow lupine Lupinus luteus L. (Juno and Taper) and narrow-leaved lupine Lupinus angustifolius L. (Baron and Karo) to feeding of the pea aphid Acyrthosiphon pisum Harris. The studies were carried out using the EPG (Electrical Penetration Graph) technique. Susceptibility of selected varieties of both lupine species was determined upon the analysis of non-sequential parameters derived from 8-hour registration of aphid probing. The results of the study revealed that two species of lupine showed a low level of susceptibility to the pea aphid feeding. This was evidenced by a short time phloem phase (less than 3% of experimental time). The varieties Karo (narrow-leaf lupine) and Taper (yellow lupine) were the least susceptible. On variety Karo, aphids did not show phloem phase. On variety Taper, aphids showed phloem phase, but the the phloem sap ingestion phase (E2) did not occur.
PL
Celem badań była ocena względnej podatności wybranych odmian łubinu żółtego Lupinus luteus L. (Taper, Juno) oraz łubinu wąskolistnego (Lupinus angustifolius L.) (Baron, Karo) na żerowanie mszycy grochowej Acyrthosiphon pisum Harris, wykonanej za pomocą techniki EPG (Electrical Penetration Graph). Podatność określono na podstawie analizy parametrów zbiorczych, uzyskanych w wyniku analizy ośmiogodzinnych rejestracji. Badania wykazały, iż oba gatunki łubinu charakteryzują się małą podatnością na żerowanie mszycy grochowej. Świadczy o tym krótki czas pobierania soku floemowego, który nie przekraczał 3% czasu ośmiogodzinnego doświadczenia. Najmniej podatne okazały się odmiany: Karo (łubin wąskolistny) oraz Taper (łubin żółty). Na odmianie Karo, u żadnej z badanych mszyc nie stwierdzono kontaktu z floemem, natomiast na odmianie Taper zarejestrowano niewielki procent mszyc, które osiągnęły floem, lecz nie stwierdzono u nich fazy żerowania E2.