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1

Dojrzewanie in vitro oocytow kota domowego

100%

Grabiec A. , Faundez R. , Max A. , Tischner M.

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tom 63

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nr 03

318-321

EN

The aim of this study was to compare the efficiency of in vitro maturation in relationship to pathomorphology of uterus and oocytes quality. Morphological evaluation of the uterus helped to classify the queens into one of three groups: those with normal uteri, those with transformed uteri and pregnant queens. Cumulus-oocyte complexes were separated at recovery into three types according to pigmetation, uniformity and smoothness of ooplasm, compactness and number of layers of cumulus cells, as well as integrity of zona pellucida. Oocytes were maturated for 36 hours at 38°C in M199 medium containing cysteine and 17b oestradiol. After IVM, the meiotic status of oocytes was determined and the percentage of matured oocytes was calculated in relationship to COCs quality and uterus condition. In the group with normal uterus, oocytes of class A maturated (63.93%) better than oocytes class B and C, although the differences in maturation of oocytes class A and B were not statistically significant. The worst results of IVM were for oocytes class C. In the group of transformed uterus, 55.17% of oocytes class A maturated, while in only 7.84% of oocytes class C metaphase II had been observed. Significant differences were established in availability to IVM between oocytes class A and C as well as class B and C. In the group with pregnant uteri, the highest percentage of matured oocytes was in class A (46.27%), though there are no statistically significant differences in IVM between oocytes class A and B (28.8%), and the oocytes class C maturated in only 1.82%. Only oocytes of class A and B should be qualified for IVM procedure. Pregnant queens as well as queens with transformed uteri can also serve as oocyte donors for in vitro maturation.

2

Zastosowanie różnych płynów inkubacyjnych do kapacytacji plemników buhaja in vitro

100%

Tizol G. , Faundez R. , Olszewski A.

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tom 46

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nr 06

EN

The experiments have been carried out with a modified Tyrode’s liquid (TALP), PBS, TALP and PBS with the addition of 1 uM of epinephrin and 10 uM of hypotaurine (TALP+, PBS+) and Ringer’s solution. There were assessed a progressive movement, morphological changes of the acrosoma and the ability of spermatozoons to penetrate the eggs of the hamster. The results showed that the solutions of TALP+ and PBS+ maintained a progressive motion of spermatozoons and increased the percentage of morphological changes in the acrosoma. Following incubation in the TALP+ there was found the highest percentage of penetrated oocytes. The findings point to epinephrin and hypotaurine' as the drugs maintaining the mobility of bull spermatozoons. No correlation was found between the percentage of changes in the acrosoma and the percentage of penetrated eggs. Such correlation was observed in case of a high percentage of spermatozoons with a progressive movement.

3

Postępowanie w przypadkach obniżonej jakości nasienia psów

100%

Gizinski S. , Faundez R. , Karska D.

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nr 01

4

Przypadek operacyjnego leczenia ciąży rzekomej u kozy

88%

Siewruk K. , Lisowska B. , Faundez R. , Pawlicki B. , Gajewski Z.

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nr 04

5

Evaluation of intra-testicular injections of calcium chloride and 4-vinylcyclohexene 1,2 monoepoxide for chemical sterilization in guinea pigs

88%

Sen C. C. , Yumusak N. , Faundez R. , Temamogullari F. , Taskin A.

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nr 2

6

Evaluation of intra-testicular injections of calcium chloride and 4-vinylcyclohexene 1,2 monoepoxide for chemical sterilization in guinea pigs

88%

Sen C. C. , Yumusak N. , Faundez R. , Temamogullari F. , Taskin A.

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nr 2

EN

This study was aimed at investigating the use of intra-testicular calcium chloride (CaCl₂) and 4-vinylcyclohexene 1,2-monoepoxide (VCM) injections as a side effect-free alternative method for the control of reproduction in guinea pigs. Fifty male guinea pigs were randomly assigned to five groups. In all groups, the chemical agents were injected into both testes in 1% lidocaine hydrochloride. While Groups I, II and III were administered with a single dose (0.25 mL) of sterile physiological saline, 15 mg/100 g CaCl₂, and 240 mg/kg VCM, respectively, Group IV and V received a daily dose of 15 mg/100 g CaCl₂, and 240 mg/kg VCM for 3 days, respectively. On day 90 post-administration, all animals were weighed and later decapitated under ether anaesthesia. Blood and tissue (testis, liver, hypophysis and adrenal gland) samples were taken. Sperm samples from the cauda epididymis were examined for spermatological parameters. Blood was used for hormone analyses and tissue samples were examined histopathologically (haematoxylin-eosin) and immunohistochemically (Tunel staining). The epididymal sperm count decreased in all treatment groups. Excluding 2 animals, Group V displayed azoospermia. When compared to the control group, Group V displayed the highest prolactin and lowest testosterone levels, and Group III showed the highest testosterone level. Histopathological examination revealed no intoxication finding. Chemical castration with VCM may be a good alternative to surgical castration as it enables mass sterilization without postoperative risks in guinea pig.