Wyniki wyszukiwania - Biblioteka Nauki

1

On the hormonal modulation of acute-phase plasma protein synthesis in perfused rat liver

100%

Koj A. , Dubin A.

Acta Biochimica Polonica

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1974

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tom 21

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nr 2

2

The intracellular serpin family

100%

Korpula-Mastalerz R. , Dubin A.

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tom 43

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nr 3

EN

The serpins are widely distributed, structurally related family of proteins with diverse functions. Most of the known serpins are proteinase inhibitors, the majority being found as secreted species, however, there are a few that occur intracellularly and their physiological role remains unknown. Most of the intracellularly occuring serpins have been classified into the ovalbumin subfamily. The possible phytogenetic tree of 14 intracellular serpins is presented.

3

Elastinolytic activity of horse leukocyte proteinases. Comparison with elastases from human leukocytes and porcine pancreas

80%

Potempa J. , Korzus E. , Dubin A. , Silberring J.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

4

Comparison of specificity of human and horse leucocyte proteinases with synthetic peptide substrates

80%

Dubin A. , Potempa J. , Schnebli H. P. , Koj A.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

5

The interaction between some serine proteinases and horse leucocyte inhibitor

80%

Dubin A. , Potempa J. , Turyna B.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 2

6

Evidence for the presence of different alpha-1-proteinase inhibitor genes products in mouse plasma

80%

Dubin A. , Mak P. , Travis J.

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tom 43

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nr 3

EN

The plasma alpha-l-proteinase inhibitor (API) of three mouse species Mus domes- ticus, M. caroli and M. pahori was isolated. Each of the species isoforms were then separated by chromatofocusing; however, no significant differences in association rate constants toward human neutrophil elastase and bovine chymotrypsin were observed. The amino-acid sequence of the P'i-P'15 C-terminal fragments of the API variants indicate that mouse plasma contains at least two different active API isoforms in the case of M. domesticus (five API genes) but only one active API isoform in M. pahori and M. caroli (one API gene).

7

Biosythesis and distibution of leucocyte elastase inhibitor. Production of recombinant in inhibitor

80%

Kasza A. , Korpula-Mastalerz R. , Rose-John S. , Dubin A.

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tom 43

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nr 3

EN

The horse leucocyte elastase inhibitor (HLEI), present in neutrophils, monecytes and bone marrow cells, is apparently a cytoplasmic protein which is not released from cells even in response to stimulation with lipopolysaccharide, phorbol ester, tumour necrosis factor alpha, interleukin-1 or elastin degradation products. Although no expression of the inhibitor was detected in neutrophils, both monocytes and bone marrow cells were efficient in its synthesis. Using a new expression vector pREST5d, recombinant inhibitor was produced in a large quantity in a soluble form, with a yield of 88 mg per 10 litres of E. colt culture. A two-step purification procedure, consisting of ion-exchange chromatography and gel filtration, yielded 36 mg of the recombinant inhibitor of a purity higher than 95%, as judged by SDS/PAGE. The recombinant protein had physicochemical and kinetic properties indistinguishable from those of the natural one, including irreversible elastase inhibition with an association rate constant kass > 10 7 M -1 s -1. Both proteins were eliminated from rat circulation at the same ratio, and within the first 20 min 70% of the protein was removed. Such a short half-life in the circulation suggests that local delivery of HLEI directly to lungs in the form of aerosol could be a more efficient therapeutic approach than its intravenous injection.

8

Evaluation of P1' substrate specificity of staphylococcal SplB protease

71%

Pustelny K. , Stach N. , Wladyka B. , Dubin A. , Dubin G.

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2014

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tom 61

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nr 1

EN

Staphylococcus aureus is a dangerous human pathogen characterized by growing antibiotic resistance. Virulence of S. aureus relies on a variety of secreted and cell surface associated virulence factors among which certain proteolytic enzymes play an important role. Amid staphylococcal extracellular proteases, those encoded by the spl operon remain poorly characterized, both in terms of enzymology and their physiological role. Initial data demonstrated that Spl proteases exhibit restricted substrate specificity. This study describes development of convenient protein FRET substrates for SplB protease and characterization of the substrate preference of the protease at the P1' position. Kinetic data on hydrolysis of a panel of substrates substituted at the said position is provided.

9

The first case of a Staphylococcus pseudintermedius infection after joint prosthesis implantation in a dog

61%

Miedzobrodzki J. , Kasprowicz A. , Bialecka A. , Jaworska O. , Polakowska K. , Wladyka B. , Dubin A.

Polish Journal of Microbiology

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2010

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tom 59

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nr 2

133-135

EN

We have reported a bacterial infection in a dog with progressive dysplasia of the hips. Orthopedic surgery was performed. Seven weeks prior to the surgery, the patient was bitten by another dog. The postimplantation wound exuded for four days after the surgery. Microbiological analysis performed by standard identification techniques showed the presence of Staphylococcus intermedins, but an additional molecular analysis indicated S. pseudintermedius. This was followed by an evaluation of antibiotic susceptibility of the strain which showed cefoxitin, ciprofloxacin, clindamycin, trimethoprim/sulfamethoxazole, doksycycline, erythromycin, and gentamicin resistance. Minimal inhibitory concentration (MIC) values for selected antibiotics were reported. Resistance for cefoxitin indicates that methicillin resistant S. pseudintermedius (MRSP) strains were present in individual macroorganisms, but they can expand and persist the colonization of other hosts.

10

Alpha-1-acute phase globulin in the blood of tunicamycin-injected rats. Isolation of the non-glycosylated form, its inhibitory properties and synthesis in liver slices

61%

Koj A. , Bereta J. , Dubin A. , Kurdowska A. , Chindemi P. , Regoeczi E.

Folia Histochemica et Cytobiologica

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1986

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tom 24

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nr 1

11

A novel member of the thermolysin family, cloning and biochemical characterization of metalloprotease from Staphylococcus pseudintermedius

61%

Wladyka B. , Bista M. , Sabat A. J. , Bonar E. , Grzeszczuk S. , Hryniewicz W. , Dubin A.

Acta Biochimica Polonica

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2008

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tom 55

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nr 3

525-536

EN

Thermolysins constitute a family of secreted bacterial metalloproteases expressed, among others, by several pathogens. Strains of Staphylococcus pseudintermedius isolated from diseased dogs and judged as protease-positive, by skim milk agar plate culture, were investigated for protease content. No proteolytic activity was detected when the bacteria were grown in regular liquid media. Unexpectedly, supplementation of the medium with calcium ions resulted in expression of a metalloprotease and profound changes in the profile of extracellular proteins. On the basis of homology to other staphylococcal metalloproteases, the nucleotide sequence of the gene encoding this protease (Pst) and its flanking regions was determined. The full-length pstcodes for a protein with an open reading frame of 505 amino acids. The internal region contains the HEXXH catalytic domain that is conserved in members of the thermolysin family. Regardless of the presence of calcium in the medium, the expression of the protease gene was of the same intensity. This suggests that regulation of the metalloprotease production by calcium ions is at a post-transcriptional level. Isolates of S. pseudintermediusexhibit a proteolytic phenotype due to the metalloprotease expression, however only in presence of calcium ions, which most probably stabilize the structure of the protease.

12

Comparative analysis of RCAS1 level in neoplasms and placenta

61%

Wicherek L. , Dutsch M. , Mak P. , Klimek M. , Skladzien J. , Dubin A.

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nr 4

EN

The tumor associated antigen RCAS1 (receptor-binding cancer antigen expressed on SiSo cells) expressed with high frequency in various cancer and trophoblast cells, inhibits growth of estrogen receptor-expressing cells and induces apoptosis. Because previous reports demonstrated RCAS1 presence only by non-quantitative immuno- cytochemistry methods, we decided to use a Western blotting with anti-RCAS1 monoclonal antibodies for estimation of the relative content of the tumor-associated antigen. One hundred tissue samples were assayed (neoplasms, chronic inflammatory diseases, healthy tissues, trophoblasts and placentas at term). RCAS1 was present in all neoplastic, placental and trophoblast tissue samples and its level in malignant samples was statistically significantly higher than in benign neoplasms. The amount of RCAS1 in chronic inflammations was also significantly increased in immune mediated diseases, like allergic nasal polyps and sarcoidosis. The RCAS1 protein was not revealed in healthy mucous membrane and in muscle tissues. The presented results suggest that RCAS1 might play an important role in tumor escape from host immunological surveillance and carry weight in the down regulation of the maternal immune response, thereby maintaining pregnancy.

13

New generation of peptide antibiotics

51%

Dubin A. , Mak P. , Dubin G. , Rzychon M. , Stec-Niemczyk J. , Wladyka B. , Maziarka K. , Chmiel D.

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nr 3

EN

The increasing antibiotic resistance of pathogenic bacteria calls for the development of alternative antimicrobial strategies. Possible approaches include the development of novel, broad-spectrum antibiotics as well as specific targeting of individual bacterial virulence factors. It is impossible to decide currently which strategy will prove more successful in the future since they both promise different advantages, but also introduce diverse problems. Considering both approaches, our laboratory's research focuses on the evaluation of hemocidins, broad-spectrum antibacterial peptides derived from hemoglobin and myoglobin, and staphostatins, specific inhibitors of staphopains - Staphylococcus aureus secreted proteases that are virulence factors regarded as possible targets for therapy. The article summarizes recent advances in both fields of study and presents perspectives for further development and possible applications.

14

Kierunki studiów biotechnologicznych w Polsce akredytowane przez Uniwersytecką Komisję Akredytacyjną (UKA)

41%

Skladanowski A. C. , Bednarski W. , Bielecki J. , Bielecki S. , Dlugonski J. , Dubin A. , Fiedurek J. , Gabrys H. , Janska H. , Kur J. , Skorupska A. , Szweykowska-Kulinska Z. , Turkiewicz M. , Wojtaszek P. , Zabza A. , Podhajska A. J.

Biotechnologia

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2003

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nr 2