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Uzyskiwanie roślin transgenicznych wolnych od genów selekcyjnych

100%

Zuzga S. , Burza W. , Malepszy S.

nr 4

Specific phosphorylation of conserved eukaryotic protein SGT1 is an essential element of plant pathogen defense

100%

Zuzga S. , Hoser R. , Hennig J. , Krzymowska M.

tom 51

nr 2

Molecular characterization of SCARECROW (CsSCR) gene expressed during somatic embryo development and in root of cucumber (Cucumis sativus L.)

75%

Wisniewska A. , Pietraszewska-Bogiel A. , Zuzga S. , Tagashira N. , Lotocka B. , Malepszy S. , Filipecki M.

Acta Physiologiae Plantarum

2013

tom 35

nr 05

Somatic embryogenesis (SE) in plants can be used as a model for studying genes engaged in the embryogenic transition of somatic cells. The CsSCARECROW (CsSCR) gene was previously identified among a panel of genes upregulated after the induction of SE in cucumber (Cucumis sativus). The putative CsSCR protein contains conserved GRASfamily domains and is extremely similar toAtSCRfrom Arabidopsis thaliana. SCR proteins are transcription factors involved in root radial patterning and are required for maintenance of the quiescent centre and differentiation of the endodermis. In comparison with other GRAS proteins from cucumber, phylogenetic analyses showed that CsSCR belongs to the SCR cluster. Increased CsSCR transcript accumulation was detected in somatic embryos and roots. Southern blot analysis and screening of the draft version of the cucumber genome confirmed the lack of close homologues in this species. CsSCR transcripts were localized by in situ hybridization in undifferentiated cells in the globular and heart stages of somatic embryogenesis, and in the endodermis of torpedo and cotyledonary stage somatic embryos, and developing primary and lateral roots. This localizationwas supported by the pattern of reporter gene activity driven by the CsSCR promoter in transgenic cucumber organs.These results suggest thatCsSCR is likely to act in tissue radial organization during somatic embryogenesis and root development.

Developmental expression of the cucumber Cs-XTH1 and Cs-XTH3 genes, encoding xyloglucan endotransglucosylase/hydrolases, can be influenced by mechanical stimuli

63%

Malinowski R. , Fry S. C. , Zuzga S. , Wisniewska A. , Godlewski M. , Noyszewski A. , Barczak-Brzyzek A. , Malepszy S. , Filipecki M.

Acta Physiologiae Plantarum

2018

tom 40

nr 07