Wyniki wyszukiwania - Biblioteka Nauki

1

Expression of p16 (INK4a), cytokeratin 19, and Ki-67 in canine laryngeal squamous cell carcinoma

100%

Gandefer-Gola M. , Ciaputa R. , Nowak M. , Madej J. A.

Bulletin of the Veterinary Institute in Puławy

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2013

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tom 57

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nr 3

EN

The study aimed at morphological and immunohistochemical characteristics of laryngeal squamous cell carcinomas and their metastases in canine lymph nodes and lungs. Tissue sections were stained using classical technique with haematoxylin and eosin. In addition, immunohistochemical studies were performed with p16, cytokeratin, and Ki-67 antibodies. An expression of all examined antigens was detected in laryngeal tumours, while in tumour metastases only expression of p16 protein and cytokeratin was demonstrated. The results pointed to higher proliferative potential of the primary tumour than of their metastases.

2

Usefulness of immunohistochemical indicators for diagnosis and prognosis of poorly differentiated tumours

100%

Kandefer-Gola M. , Nowak M. , Ciaputa R. , Madej J. A.

Journal of Veterinary Research

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2016

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tom 60

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nr 3

3

Manifestation of tumours in domestic animals in Lower Silesia in 2005-2008

100%

Nowak M. , Madej J. A. , Ciaputa R. , Poradowski D.

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2010

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tom 54

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nr 2

229-236

EN

The aim of this research was to analyse the frequency of prevalence and localisation of neoplasms in domestic animals. The material consisted of 1,958 tumours, originating from the area of Lower Silesia, and affecting dogs, cats, horses, cattle, and exotic animals, sampled during the surgery, autopsy or biopsy, and submitted for histopathological diagnosis. Tumours of dogs were the most frequent - 1,700 cases (87%), followed by tumours of cats - 207 cases (10.6%), horses - 20 cases (1%), cattle - 3 cases (0.2%), and exotic animals - 20 cases (1.2%) including mostly tumours of ferrets - 8 cases (0.4%). In relation to similar studies carried out in the same area during 1957-1995 and 2000-2004, an increase in neoplasms in domestic animals was observed. Skin and subcutaneous tissue neoplasms and mammary gland neoplasms (principally adenocarcinomas) dominated.

4

Prevalence of inclusion body hepatitis (IBH) in Poland from 2010-2014

100%

Gawel A. , Nowak M. , Ciaputa R. , Bobrek K.

Polish Journal of Veterinary Sciences

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2016

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tom 19

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nr 4

EN

This study investigated the prevalence of inclusion body hepatitis (IBH) infections during the past five years (2010-2014) in broiler chicken flocks in Poland. The diagnosis of IBH was based on anatomopathological investigations and histopathological analyzes. IBH was the most prevalent disease in broiler flocks in Poland (10.4%) during this time period, and occurred as a primary infection. The fewest cases of IBH occurred in the summer, suggesting that the temperature may have been a possible stressor that increased susceptibility to IBH. However, over the last 5 years the occurrence of clinical IBH cases in Poland has systematically decreased.

5

Prevalence of tumours in domestic animals in the Lower Silesia (Poland) in 2009–2011

100%

Ciaputa R. , Kandefer-Gola M. , Nowak M. , Madej J. A.

Bulletin of the Veterinary Institute in Puławy

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2013

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tom 57

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nr 1

EN

The study aimed at the analysis of prevalence frequency and localisation of tumours in domestic animals. The research material comprised 4,212 tumours developed in dogs, cats, horses, and exotic animals, isolated during surgery , autopsy or biopsy, performed for the purpose of histopathological diagnosis. The most numerous group involved canine tumours, including 3,585 cases (85.1%), followed by tumours in cats (532 cases, 12.6%), ferrets (34 cases, 0.81%), rats (19 cases, 0.45%), horses (15 cases, 0.36%), and rabbits (14 cases, 0.33%). A significant increase in incidence of tumours was noted, as compared to studies performed in the same region of Poland in 1957-1995 and 2000-2004 or in 2005-2008. This was particularly evident in exotic animals, in which 80 cases (1.9%) were detected in 2009-2011 but only 20 cases (1.2%) in 2005-2008. The most frequent localisation of neoplasia involved the skin, subcutaneous tissue, and mammary gland.

6

Cardiomyocyte marker expression in dogs with left atrial enlargement due to dilated cardiomyopathy or myxomatous mitral valve disease

75%

Janus I. , Kandefer-Gola M. , Ciaputa R. , Noszczyk-Nowak A. , Paslawska U. , Tursi M. , Nowak M.

Folia Histochemica et Cytobiologica

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2017

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tom 55

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nr 2

7

Morphological and immunohistological characteristics of follicular-compact thyroid carcinoma in dog

75%

Ciaputa R. , Nowak M. , Kandefer-Gola M. , Dziedzic K. , Halupka P. , Pula B. , Kielbowicz M.

Folia Histochemica et Cytobiologica

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2014

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tom 52

|

nr 2

8

Analysis of the expression of N-cadherin and survivin in an established D-17 cell line and in canine cells of a spontaneous osteosarcoma

75%

Ciaputa R. , Poradowski D. , Janus I. , Nowak M. , Obminska-Mrukowicz B. , Madej J. A. , Kandefer-Gola M.

Medycyna Weterynaryjna

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2015

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tom 71

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nr 06

EN

The aim of the study was to demonstrate and evaluate the expression of N-cadherin and survivin in spontaneous osteosarcoma tumours in dogs compared to the expression of these proteins in an established (D-17) cell line of canine osteosarcoma. Osteosarcoma samples were taken from the limbs of 15 dogs and fixed in 7% buffered formalin. An adherent canine osteosarcoma cell line (D-17) was also used for the study. The cytoplasmic expression of N-cadherin and survivin was shown in spontaneous osteosarcoma, whereas the cytoplasmic and nuclear expression of N-cadherin and survivin was observed in cells derived from the D-17 cell line. The results indicate that the higher the cytoplasmic expression of N-cadherin, the higher the expression of survivin. By analyzing the strength of intercellular adhesion, which is based on the type and strength of N-cadherin expression, as well as the degree of apoptosis inhibition, one may conclude that these markers may be used as supplementary to routine tests to evaluate the degree of the tumour’s malignancy and the patient’s prognosis.

9

Immunohistochemical evaluation of neoangiogenesis in canine mast cell tumours

75%

Kandefer-Gola M. , Madej J. A. , Dzimira S. , Janus I. , Nowak M. , Ciaputa R.

Bulletin of the Veterinary Institute in Puławy

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2015

|

tom 59

|

nr 2

EN

The aim of the study was to assess the microvessel density based on the analysis of the expression of the CD31, VEGF, and LIMS-1 proteins in canine mast cell tumours. The study was conducted on 60 mastocytomas; 16 cases were classified as the grade I, 26 as the grade II, and 18 as the grade III. Statistical analysis showed a positive correlation only between the grade of the tumour and the expression of LIMS-1. In conclusion, LIMS-1 could be successfully used as a prognostic endothelial cell marker in mast cell tumour. CD31 may be a useful marker, but further examinations are necessary. VEGF is not recommended.

10

Adenocarcinoma of the posterior segment of the gastrointestinal tract in dogs - clinical, endoscopic, histopathological and immunohistochemical findings

75%

Spuzak J. , Ciaputa R. , Kubiak K. , Jankowski M. , Glinska-Suchocka K. , Paradowski D. , Nowak M.

Polish Journal of Veterinary Sciences

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2017

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tom 20

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nr 3

EN

Of all the tumours in dogs, three percent are located in the intestines, and 36-60% of those tumours affect the large intestine. Adenocarcinomas of the intestines account for 20-35% of the gastrointestinal tumours and for almost 60% of the large intestine tumours. The aim of the study was to analyze clinical disorders and endoscopic, histopathological and immunohistochemical changes in colorectal adenocarcinomas in dogs with the use of the E-cadherin, β-catenin, cytokeratin 20 (CK20), Ki-67 and minichromosome maintenance 3 (MCM-3). The study comprised 11 dogs of both genders and of different breeds diagnosed with adenocarcinoma of the large intestine. They were from 4 to 11 years old. The large intestine adenocarcinoma was diagnosed in all the patients. 72.7% cases were diagnosed with a rectal adenocarcinoma, and 27.3% were found to have a colonic adenocarcinoma. All the studied proteins were expressed at different levels and, together with the histological findings, indicated different levels of malignancy (G). The statistical analysis revealed no statistically significant differences between the expression of E-cadherin and β-catenin in the studied tissues (p=0.79) and between the expression of Ki-67 andMCM-3 (p=0.39). A strong positive correlation was found between the expression of E-cadherin and β-catenin (r=0.86; p<0.05). The diagnosis of adenocarcinomas of the large intestine may be facilitated by the introduction of immunohistochemical studies using appropriate cell markers. They may also aid in the accurate evaluation of the biological character of the tumours, their origin, the connections between tumour cells and the mitotic index. That, in turn, may help determine the malignancy and the choice of treatment.

11

Urothelial cancer of the prostate gland. Immunohistochemical analysis of two cases of rare canine cancer

75%

Ciaputa R. , Grieco V. , Janus I. , Kandefer-Gola M. , Dziegiel P. , Nowak M.

Folia Histochemica et Cytobiologica

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2020

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tom 58

|

nr 4

12

Kostniakomięsak (osteosarcoma) kości czołowej u konia - przypadek kliniczny

75%

Ciaputa R. , Buch-Kupczyk H. , Nowak M. , Poradowski D. , Kandefer-Gola M. , Atamaniuk W. , Rozycki P.

Magazyn Weterynaryjny

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2013

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tom 22

|

nr 08

13

COX-2 and mPGES-1 expression in canine mast cell tumours

75%

Kandefer-Gola M. , Nowak M. , Dzimira S. , Janus I. , Ciaputa R. , Madej J. A.

Medycyna Weterynaryjna

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2015

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tom 71

|

nr 06

EN

COX-2 and mPGES-1 participate in the development of tumours and play a key role in their progression by impacting cell proliferation, apoptosis and neoangiogenesis. The aim of the study was to assess the expression of COX-2 and mPGES-1 in mast cell tumours. The existence of correlation between the histologic malignancy of the tumour and the expression of the proteins was verified through statistical analysis. Fifteen canine skin mast cell tumours were used in the study. Tissue sections were stained with H&E to determine their histologic grade. Subsequently, immunohistochemical staining of COX-2 and mPGES was carried out. A cytoplasmic reaction was observed in all tumours. Statistical analysis revealed a strong, positive correlation (p < 0.05) between the two markers and the grade of the tumour. Both markers may be used effectively in the diagnosis of skin mast cell tumours in dogs.

14

Rozdemowe zapalenie pęcherza moczowego u psa - opis przypadku

75%

Kandefer-Gola M. , Ciaputa R. , Nowak M. , Pozniak A. , Jaskolska A. , Pozniak B.

Medycyna Weterynaryjna

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2012

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tom 68

|

nr 08

EN

An 11-year-old, unsterilised Scottish Shepherd Collie bitch was brought to a veterinary clinic after a previous symptomatic treatment in another surgery. Clinical signs raised a suspicion of pyometra. Additional tests (radiological and ultrasound tests) revealed a change in the bladder that suggested a neoplastic process. The veterinarian decided to make a diagnostic laparotomy, during which, the animal was intraoperatively euthanised because of extensive changes in the bladder. A complete pathologic examination indicated emphysematous cystitis. Emphysematous cystitis is a rare disease in both humans and animals. It is often related to diabetes, chronic urinary tract infections, bladder stones, or the consequences of interventions on the bladder. In the bitch under examination, these changes resulted from a chronic inflammation of the bladder caused by Escherichia coli and Enterococcus faecalis, which was confirmed by bacteriological examination. The most important element for accurate diagnosis is to perform radiological examinations (X-ray, CT). Treatment mainly consists of antibiotics administered according to antibiogram.

15

Chłoniak nerki u młodego kota. Opis przypadku

75%

Borecka P. , Janus I. , Ciaputa R. , Kandefer-Gola M. , Brzozowska M. , Hildebrand W. , Nowak M.

Weterynaria w Praktyce

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2015

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tom 12

|

nr 07-08

16

Sercowa postać parwowirozy u psa. Opis przypadku

75%

Janus I. , Sobska M. , Ciaputa R. , Kendefer-Gola M. , Nowak M. , Rypula K.

Magazyn Weterynaryjny

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2015

|

tom 24

|

nr 02

17

Expression of selected markers in immunohistochemical diagnosis of canine and human testicular tumours

75%

Ciaputa R. , Nowak M. , Madej J. A. , Janus I. , Gorzynska E. , Kandefer-Gola M.

Bulletin of the Veterinary Institute in Puławy

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2015

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tom 59

|

nr 1

EN

Immunohistochemical profiles of the most common canine testicular tumours, including the Leydig cell tumours, seminomas, and Sertoli cell tumours were analysed, and the results were compared with those obtained in the corresponding txpes of human testicular neoplasms. The expressions of vimentin, von Willebrand factor (FVIII), chromogranin A, synaptophysin, and MCM3 were quantified. In the case of Sertoli cell tumours, only canine ones were analysed, since this type of tumour is very rareh diagnosed in men. The expression of the analysed proteins in the testicular tumours was similar. The von Willebrand factor exhibited the strongest expression in Lexdig cell tumours in dogs and men, while vimentin was expressed more strongly in dogs (96.7% had an intensity at +++) than in men (62.5% had +++) in the Leydigioma. The immunoexpression of MCM3 in seminomas was high in both men and dogs - 90% +++ and 100% +++ respectively. The lack of chromogranin A and synaptophysin was observed in almost 100% of seminomas in men and dogs. This differed from the results obtained for Leydigioma, where chromogranin A was expressed in 70% of dogs at +++ and in 100% of men at ++++. The results may indicate that the antibodies were selected correctly. Their anah sis and interpretation provides valuable information concerning the nature of the studied tumours.

18

Seminoma, sertolioma, and leydigoma in dogs: clinical and morphological correlations

75%

Ciaputa R. , Nowak M. , Kielbowicz M. , Antonczyk A. , Blasiak K. , Madej J. A.

Bulletin of the Veterinary Institute in Puławy

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2012

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tom 56

|

nr 3

EN

The study aimed at presenting the most frequent male gonadal tumours in dogs, their clinical and histopathological aspects, at outlining aetiopathogenesis and differential diagnosis of the tumours. As examples of the most frequently manifested testicular tumours, three clinical cases were presented, involving tumour of interstitial (Leydig) cells, tumour of Sertoli cells, and seminoma. Respective clinical diagnosis employed USG, X-ray patterns, and morphological and biochemical tests. The surgically sampled material was stained with H+E and an attempt was made to establish expression of E-cadherin, calretinin, and Ki-67. It was shown that histopathological diagnosis of testicular tumours in dogs is frequently very difficult and complex and requires multidirectional studies.

19

Comparative analysis of markers of cell proliferation in canine mast cell tumours according to current classifications

75%

Kandefer-Gola M. , Madej J. A. , Dzimira S. , Nowak M. , Janus I. , Ciaputa R.

Polish Journal of Veterinary Sciences

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2015

|

tom 18

|

nr 2

EN

The study aimed at immunohistochemical analysis of various markers of cell proliferation and comparison of the results with canine mast cell tumours grading systems according to the Patnaik and Kiupel. Tissue sections were stained using classical technique with haematoxylin and eosin, and immunohistochemical studies were performed with Ki-67, PCNA and MCM-3 antibodies. Additionally the mitotic index was assessed. Statistical analysis including rank correlation Spearman's and ANOVA Friedman analysis was performed. The significance was set at p<0.05. Expression of all examined antigens was detected. The results obtained allow concluding that there is a strong relationship between all the cell markers. However, due to the very strong response and positive reaction in the majority of tumours PCNA is not recommended as a prognostic indicator. Ki-67 and MCM-3 can be successfully used in the evaluation of canine mast cell tumours.

20

Wrodzony brodawczak skóry u źrebięcia - opis leczenia z zastosowaniem chirurgicznego lasera CO2

63%

Siwinska N. , Zak A. , Slowikowska M. , Niedzwiedz A. , Sapikowski G. , Ciaputa R. , Nowak M. , Popiel J.

Medycyna Weterynaryjna

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2019

|

tom 75

|

nr 07

EN

Skin papilloma in humans and animals is a relatively common dermatological problem. The most common form of these changes are acquired papillomas, and while congenital ones are rare, they were most often described in humans and horses. The present article describes the first case in Poland of congenital single skin papilloma in a newborn thoroughbred foal, surgically removed using a CO₂ laser. The treatment was carried out after 3 months from birth when the change began to progress. Removal was performed on the standing animal with pharmacological sedation. Histopathological examination confirmed the diagnosis. The convalescence process proceeded without complications, with quick healing and no scar remaining.