Wyniki wyszukiwania - Biblioteka Nauki

1

Response of plasma lipids and biliary secretion to duodenal infusion of rape oil and glucocorticoid administration in calves

100%

Bobowiec R. , Martelli F. , Sigheri C.

Annales Universitatis Mariae Curie-Skłodowska. Sectio DD:Medicina Veterinaria

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1994

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tom 49

59-67

2

Wspolzaleznosc miedzy losem dojrzalych pecherzykow jajnikowych a profilem hormonalnym i poziomem IGF-I u klaczy

100%

Bobowiec R. , Kedzierski W. , Martelli F. , Kosior-Korzecka U.

Medycyna Weterynaryjna

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2004

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tom 60

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nr 10

1098-1102

3

Osoczowa leptyna u owiec z pojedyncza i podwojna owulacja

100%

Bobowiec R. , Kosior-Korzecka U. , Martelli F.

Medycyna Weterynaryjna

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2001

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tom 57

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nr 02

122-126

4

Leptin influences histidine dipeptides and nitric oxide release from anterior pituitary cells of sheep in vitro

100%

Kosior-Korzecka U. , Ducci M. , Martelli F. , Bobowiec R.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 9

19-27

5

The role of reduced glutathione in bile formation in sheep

100%

Bobowiec R. , Kosior-Korzecka U. , Martelli F. , Sighieri C.

Polish Journal of Veterinary Sciences

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1999

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tom 02

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nr 1

EN

The present study reports the investigation on the influence of reduced glutathione (GSH) on bile secretion in 4 canulated sheep during constant intraduodenal infusion of bile alone, bile with sodium taurocholate (TCHNa) or bile with sodium deoxycholate (DCHNa). Infusions of bile alone and bile with TCHNa or DCHNa brought about an increase in the bile salts secretion from 0.05 ± 0.01 (μM/min/kg to the value of 0.12 ± 0.03, 0.22 ± 0.07 and 0.32 ± 0.08 μM/min/kg, respectively. It was shown that the administration of GSH during the infusion of both bile alone and bile+bile salts slightly but significantly (p < 0.05) lowered bile salts secretion. At the same time both bile with glutathione and bile+bile salts+glutathione increased the bile flow significantly (p < 0.05) as compared to that found after infusions without glutathione. After the i.v. administration of GSH, the volume of the bile increased to a lesser extent than it did with bile+TCHNa or, especially, with bi- le+DCHNa infusions. The concentration of glutathione in the bile was the highest during the infusion of bile+DCHNa (671.18 ng/ml), lower during the infusion of bile+TCHNa (471.40 ng/ml) and the lowest during the infusion of bile alone. Although the bile salts secretion decreased during the simultaneous infusion of the bile with bile salts and glutathione, there was an increase in the bile flow. Therefore, we concluded that some bile acid independent fraction was secreted into the bile and that the fraction was dependent on GSH secretion at the level of canaliculi.

6

Effect of carotenoids on in vitro proliferation and differentiation of oval cells during neoplastic and non-neoplastic liver injuries in rats

100%

Wojcik M. , Bobowiec R. , Martelli F.

Journal of Physiology and Pharmacology. Supplement

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2008

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tom 59

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nr 2

EN

The goal of this study was to investigate the effects of ß-caroten and astaxanthin (ASX) - carotenoid without provitamin A activity on the proliferation and differentiation of rat oval cells (OC) in vitro. Oval cells were isolated from two groups of animals: I - partial hepatectomised (PH) and II- diethylnitrosamine (DEN) treated rats. At various time points cell lysates were separated by PAGE. For immunodetection primary antibodies against CD-34, Ck19 and albumin were used. Medium concentration of fibrinogen and haptoglobin was measured. Mitochondrial competence of cells was expressed as the proliferation index. In comparison to HP- and DEN-obtained oval cells cultured without carotenoids, the addition of ß-caroten and ASX increased albumin expression during the experimental period. The same condition didn't reveal CK19 expression. CD34 expressed by oval cells was detected up to the 5th week of ß-caroten and ASX absence in the medium. ß-carotene addition resulted in a decrease of the proliferative activity of OC, with significant changes in 48h, the 5th and 15th week of incubation. ASX (p 0.05) inhibited the proliferation, especially in 24h and 5th week of cell culture. In respect to haptoglobin concentration, its maximum value after the 10th week was observed. The fibrinogen level obtained from DEN-oval cells incubated with ß-caroten elevated from 480±6.87 µg/ml after 24h to 5520±34,56 µg/ml after the 15th week. In a condition without carotenoids fibrinogen concentration did not exceed 2280±31.5 µg/ml after the 15th week of cell culture.

7

Gallbladder pressure changes induced by caerulein, pilocarpine and bile salts in calves measured by radiotelemetry

100%

Bobowiec R. , Martelli F. , Sighieri C. , Borowicz P.

Polish Journal of Veterinary Sciences

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2000

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tom 03

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nr 3

EN

Four calves were implanted a micro-radio-pressure capsule into their gallbladders in order to measure continuously pressure changes over 3 months. A special programme was designed to compute an electric signal on pressure value expressed in mm Hg. During fasting state, permanent rhythmic pressure changes were superimposed on a cyclic tonic increase in the gallbladder pressure. While rhythmic pressure changes at a frequency of 4,0 ± 0,7 cycles/10 min lasted usually 90,00 ± 7,00 s, the duration of tonic pressure changes was around 24,00 ± 2,90 min being repeated every 95,00 ± 7,00 min. After feeding, the rhythmic pressure changes exhibited a greater amplitude, and occasional long duration of a tonic increase in pressure was prolonged up to 110 min. Caerulein (1 Hg/kg) significantly increased tonic pressure changes. The most abundant response of the gallbladder was seen during the first 20 min of postinjection period, indicating direct actions on its musculature. When injecting pilocarpine (1 mg/kg) of cholinergic potency, the tonic response was more dilated and of a smaller amplitude, but rhythmic pressure changes were distinct. Infusion of 30 μM/min/20 min TCHNa (sodium taurocholate) induced a two phasic increase in gallbladder pressure lasting 35,90 ± 4,70 min. These data suggest that long duration of tonic pressure changes of the gallbladder are controlled by the hormonal, CCK dependent mechanism, but permanent rhythmic pressure changes are influenced by vagal stimulation. It also appears that bile salts can modulate pressure changes of gallbladder, especially trihyd- roksy-derivatives in the form of TCHNa, but the mechanism by which bile salts exert a contractile effect remains to be elucidated.

8

Disturbances of cow oocyte maturation by phytoestrogens

88%

Borzym E. , Bobowiec R. , Kosior-Korzecka U. , Martelli F. , Burmanczuk A.

Medycyna Weterynaryjna

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2008

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tom 64

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nr 09

EN

The aim of the study was to elucidate the influence of different phytoestrogens (PEs) such as izoflavons (represented by genistein), stilbens (represented by resveratrol (RES)), lignans (represented by enterodiol) and coumestans (represented by coumstrol) on the maturation process of isolated bovine oocytes. We assumed that their influence on the rate of oocyte growth and maturation would be related primarily to their estrogenic activity (isoflavones and coumestans are similar to the 17β estradiol - E₂, the most potent oestrogen). Eighty to one hundred oocytes were cultured in 4 ml of the medium in six-well multidishes. Cumulus oocyte complexes (COCs) were matured at 39°C in humidified air containing 5% CO₂ for 24 hours. Four phytoestrogens were tested at two concentrations: 10 µg/ml and 100 µg/ml. These results clearly show that there is no significant relation between PEs and the survival of cow oocytes. However, under the influence of different PEs the expansion of oocytes was suppressed, especially when genistein and coumestrol were introduced into the culture medium. It was noted that the addition of the phytoestrogens into the medium decreased the rate of oocyte maturation. The highest increase of immature oocytes (p ≤ 0.05) was noted after the addition of coumestrol (30.77%) and genistein (20%) (fig. 4). The most pronounced suppression of oocyte maturation by genistein and coumestrol observed in our research indicates, therefore, that there is a direct relationship between the potency of the estrogenic activity of particular PEs and the inhibition of oocyte maturation. We concluded from our studies that PEs may disturb the conception and fertilisation rate in the cow by prolonging cow oocyte maturation.

9

Wplyw Vetaheparu na wydzielanie zolci u owiec

88%

Bobowiec R. , Gluszak J. , Martelli F. , Wojcik M. , Wessely-Szponder J.

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tom 63

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nr 08

979-981

EN

The effect of Vetahepar (VH) (active ingredient: menbutone), given per os (p.o.) or i.v., on volume and biliary bile salt secretion was investigated on four sheep, which were cannulated for choledochus and duodenum. The dose of VH 20 mg/kg m.c. given p.o. was most effective in the stimulation of bile salts secretion and volume of bile. The choleretic effects of VH began 30 minutes after oral administration and peaked at 60 minutes. The augmented volume of bile occurred earlier than the increase in bile salt secretion. Such a response can suggest that the relaxation of the vascular bed in the liver under the influence of VH stimulation anticipates events evoked by bile salt stimulation. Furthermore, a considerably higher response to the same dose of VH has been observed when only 50% of the bile returned to the duodenum. Thus beneficial effects of VH are especially revealed when disturbances in liver functioning occur.

10

Assessment of neutrophil components as markers of lung injury in the course of bovine respiratory tract infections

88%

Wessely-Szponder J. , Bobowiec R. , Martelli F. , Wojcik M. , Kosior-Korzecka U.

Polish Journal of Veterinary Sciences

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2004

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tom 07

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nr 3

EN

To define the role of activated neutrophils in lung injury during bovine respiratory tract infections (BRTI) their in vitro function was investigated. As a means to achieve this goal the comparison of secretory action between neutrophils from the BRTI group and control was made on the basis of elastase, myeloperoxidase (MPO), alkaline phosphatase (ALK-P) release, and nitric oxide production. We noted that there is an interdependence between secretory response of neutrophils and clinical severity of BRTI. The release of elastase was greater in the BRTI group than in the control group (49.17 ±4.41 versus 46.43 ±4.95% of the total content). Neutrophils from infected heifers exhibited a significantly (p<0.05) higher value of MPO release than from healthy heifers and reached 39.23 ± 10.18 versus 25.54 ± 8.41% of the total content. ALK-P containing granules released significantly (p<0.001) more enzyme in the group with BRTI than in the control group (22.42 ± 6.27 versus 13.74 ± 2.01% of the total enzyme content). The level of nitrite accumulation rose in the culture of cells isolated from heifers with BRTI from 4 ± 0.53 μM after 0.5h to 6.9 ± 0.52 μM after 72 h. Our data suggest that during BRTI the increase of neutrophil secretory action results in augmentation of enzyme release including elastase, MPO and ALK-P, and the nitrite production. During an excessive secretory response of neutrophils all these factors contribute to lung injury and worsen the course of a disease and might be recognised as markers of lung injury. Moreover, such a destructive action of neutrophils must be taken into account during the introduction of new methods of BRTI treatment.

11

Influence of diet cation-anion difference (DCAD) on plasma acid-base status in pregnant sheep

75%

Wojcik M. , Martelli F. , Bobowiec R. , Patkowski K. , Chalabis-Mazurek A. , Walkuska G.

Medycyna Weterynaryjna

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2009

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tom 65

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nr 10

EN

The last trimester of pregnancy in sheep is frequently associated with pregnancy toxemia, usually connected with ketonemia and metabolic acidosis. Because a changed diet cation-anion difference (DCAD) is able to change the values of plasma acid-base balance (ABB) the authors sought to determine what kind of shift in ABB values is induced by diets with different DCAD. The authors also compare the influence of two diets with a different DCAD on some reproduction parameters in sheep. Although both diets used in our experiment had a positive DCAD, one of them was more alcalemic (high-DCAD) and the other more acidemic (low-DCAD). In the group of sheep fed with a +214.5 mEq/kg DM DCAD diet, blood pH was higher than under +46.2 mEq/kg DM condition. Significant changes, especially in the 15th and 16th week of pregnancy were observed. Under a high-DCAD condition, anion gap (AG) was elevated to 19.6 ± 2.62 mEq/l. No significant changes of AG in low-DCAD fed animals were observed. The values of AG in this group ranged between 18.02 ± 3.68 mEq/l - 19.15 ± 1.79 mEq/l. The feeding of sheep with +214.5 mEq/kg DM essentially resulted in negative values of a strong ion gap (SIG). In the second group, fed with lower DCAD, positive SIG (3.43 ± 0.55 mEq/l) occurred already in the 16th week of pregnancy. In this group of sheep, the litter size was higher and reached 1.29 ± 0.7. By employing forage with a known value of DCAD it is possible to influence not only on the health of sheep during the course of pregnancy but also to improve their reproductive parameters.

12

Stimulation of ovulation rate in ewes by continuous oxytocin administration during oestrus

75%

Bobowiec R. , Kosior-Korzecka U. , Wojcik M. , Martelli F. , Sighieri C. , Patkowski K.

Acta Scientiarum Polonorum. Medicina Veterinaria

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2003

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tom 02

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nr 2

13

Concentrations of carnosine, anserine, L-histidine and 3-methyl histidine in boar spermatozoa and sheep milk by a modified HPLC method

63%

Ducci M. , Pacchini S. , Niccolini A. , Gazzano A. , Cerri D. , Gadea J. , Bobowiec R. , Sighieri C. , Martelli F.

Polish Journal of Veterinary Sciences

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2006

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tom 09

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nr 3

EN

The present study deals with the application ofhigh-perform ancc-liquid-chromatographyl(HPLC) method for a quantitative detection of carnosine, anserine, L-histidine and 3-methyl-L-histidine in biological material with o-phthaldialdehyde (OPA) post column derivatisation at the constant temperature of 50°C. For this purpose, some mobile-phases were prepared with scalar acetonitrile concentrations. A complete separation of all molecules, particularly for carnosine and 3-methyl-L-his- tidine, was obtained with a solution of acetonitrile and 6mM hydrochloric acid with 0.48 M sodium chloride (5%:95% v/v). Post column derivatisation reaction at temperature of 50°C permitted to obtain an increase in sensibility of all molecules. This method has been utilised for detection of histidine dipeptides in boar spermatozoa and in sheep milk. Concentrations (mean ± S.E. nmol/109 spermatozoa) of carnosine (0.96 ± 0.14) and anserine (0.83 ± 0.18) in boar spermatozoa were significantly lower than those of L-histidine (52.85 ± 4.86) and 3-methyl-L-histidine (83.07 ± 7.1). Positive correlation was found between carnosine and anserine contents (r=0.740; p<0.01) and between L-histidine and 3-methyl-L-histidine (r=0.657; p<0.01). All histidine dipeptides studied were also present in 40 samples of sheep milk. In a case of samples without unit-forming colonies (UFC) of Staphylococcus coagulase-positive, carnosine concentrations (9.17 ±0.89 nmol/ml) were higher than anserine (0.51 ±0.02 nmol/ml) and both were significantly lower in respect to L-histidine (49.51 ± 6.48 nmol/ml) and 3-metyl-L-histidine (81.21 ±6.82 nmol/ml). A negative correlation was observed between carnosine milk levels (r=-0.773; p<0.01) and UFC/ml of Staphylococcus coagulase-positive. In conclusion this very simple and fast method can be used to detect histidine dipeptides in biological compartments where their concentrations are very low.