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Identification of the characteristics of a carbon dioxide breathing rotary sliding-vane expander

100%

Milewski J. , Lis P. , Olszewski A. , Sieńko A. , Sęk T.

tom Vol. 97, nr 3

259--264

This paper presents a study providing the experimental analyses on the rotary vane expander operating conditions with carbon dioxide as working fluid in comparison against air (chosen as reference). Such working fluid is promising for application as energy converters in a waste heat recovery for electric power. Experiments were performed on a prototype of rotary sliding vane expander. The experiment demonstrated that commercially available multi–vane pneumatic engines can be adopted to waste heat recovery systems. During the experiments, the operation of the multi–vane expander was kept under observation for various inlet temperature, various pressure ratios, and various rotational speeds. Adequate maps of performances for air and carbon dioxide were created, compared against each other, and commented. In general, by using the same machine, power and efficiency can be raised by only change of working fluid.

Analysis of biofilm production in Enterococcus faecium strains depending on clinical source

86%

Sieńko A. , Wieczorek P. , Majewski P. , Sacha P. , Wieczorek A. , Ojdana D. , Tryniszewska E.

2017

tom 7(1)

83-89

Purpose: Enterococcus faecium strains have been reported worldwide as etiologic factors of many nosocomial infections, which are difficult to manage because of the constantly increasing resistance of these microorganisms to antibiotics and the ability to form biofilm. The aim of this study was to analyze the ability to produce a biofilm in E. faecium strains, depending on the patient’s clinical material. Materials and methods: Sixty-six E. faecium strains were investigated. Identification and susceptibility testing were conducted by the VITEK2 system. The ability to form biofilm was assessed by phenotypic methods. The presence of selected virulence genes was established by PCR followed by gel electrophoresis and sequencing. Results: Among the tested E. faecium isolates, 72.7% were biofilm-positive (BIO+) and 27.3% biofilm-negative (BIO-). Strains were collected mostly from rectal swabs (30.4%) and blood (18.3%). BIO+ strains from infections constituted 31.8% (52.4% isolated from blood) and from colonization 40.9% (48.2% from rectal swabs). 91.7% of the Blood Group strains and 68.5% of the Other Group strains produced biofilm. Strains from the Colonization Group produced biofilm in a proportion similar to the Infection Group (about 75%). There were no statistically significant differences in virulence and resistance, except for vancomycin (more resistant BIO+ Other than the BIO+ Blood Group, and more resistant BIO+ Colonization than BIO+ Infection Group) and teicoplanin (more resistant BIO+ Colonization than the BIO+ Infection Group). Conclusion: The majority of E. faecium isolates carries high levels of resistance to many antimicrobials, is well equipped with virulence genes, and possesses the ability to form biofilm.