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EN
The aim of the present work was to evaluate the influence of exercise on serum homocysteine and oxidative stress in Thoroughbreds during official races. For our study, ten Thoroughbred mares, 5 years old and clinically healthy, were used. The horses were trained to take part in an official 2100 meter race. Reactive oxygen species (dROMs), antioxidant barrier (Oxy-adsorbent), thiol antioxidant barrier (SHp) and homocysteine (sHcy) were investigated. Blood samples, collected from each horse at rest, immediately after the race, and 30 and 180 min after the end of the race, were collected by jugular venipuncture using vacutainer tubes with no additive for the assessment of dROMs, Oxy-adsorbent and SHp by means of a UV spectrophotometer, while serum total Hey values were determined by high performance liquid chromatography with fluorimetric detection and isocratic elution. Statistical analysis, one way repeated measures analysis of variance (ANOVA), followed by Bonferroni's test, showed statistical differences (P<0.05 was considered statistically significant) for all parameters studied. Moreover, a coefficient of linear correlation (r) was computed for values of dROMs and sHcy for all sampling times but a significant linear regression (r = 0.94) was found only after the race. The variations of sHcy, dROMs, Oxy-adsorbent and SHp during the experimental period considered suggest the important role of oxidative status in the athletic horse. The systematic analysis of oxidative stress and its influence on homocysteine levels contribute to the clinical evaluation and assessment of the athletic performance of the horse.
EN
Assessed were changes in selected biochemical parameters taking place during different stages of pregnancy, post-partum, lactation and dry period in ewes. The observations were conducted on 10 clinically healthy Comisana ewes kept on one farm. Blood samples were withdrawn once before the morning feeding during dioestrus phase before pregnancy, and then on week 4, 11 and 18 of pregnancy, week 2 post-partum, week 5, 15 and 25 of lactation and finally once in a dry period (week 32). Blood serum total protein as well as serum albumin increased significantly during pregnancy compared to dioestrus phase. Compared to dioestrus, a significant increase during late gestation and a significant decrease during dry period was shown in blood urea. Total blood lipids showed a significant increase during pregnancy, post-partum and early lactation compared to dioestrus, while total cholesterol and triglycerides showed the opposite trend. The study showed marked changes in certain biochemical parameters of sheep blood serum during late pregnancy, post-partum, lactation and dry period.
PL
Obserwacji dokonano na 10 klinicznie zdrowych maciorkach rasy Comisana na Sycylii, utrzymywanych na jednej farmie. Próbki krwi pobrano jednorazowo od owiec jałowych w fazie oestrus,a dalej w 4, 11 i 18 tygodniu ciąży, w 2 tygodnie po wykocie, w 5, 15 i 25 tygodniu laktacji i jednorazowo po zasuszeniu. Zawartość białka całkowitego i albuminy w surowicy podczas ciąży okazała się istotnie wyższa niż w fazie dioestrus. W okresie późnej ciąży stwierdzono, w porównaniu z dioestrus, istotny wzost zawartości mocznika w surowicy, a w okresie zasuszenia istotny jego spadek. Zawartość lipidów całkowitych podczas ciąży, po wykocie i we wczesnej laktacji była istotnie wyższa niż w dioestrus,podczas gdy w przypadku cholesterolu całkowitego i trójglicerydów stwierdzono zjawisko odwrotne.
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