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Leptin influences histidine dipeptides and nitric oxide release from anterior pituitary cells of sheep in vitro

100%

Kosior-Korzecka U. , Ducci M. , Martelli F. , Bobowiec R.

Journal of Physiology and Pharmacology. Supplement

2008

tom 59

nr 9

19-27

Zastosowanie modelu Stewarta do analizy zaburzen rownowagi kwasowo-zasadowej u psow z przewlekla niewydolnoscia nerek

75%

Marczuk J. , Filar J. , Ducci M. , Bobowiec R. , Kosior-Korzecka U. , Nowakowski H.

tom 62

nr 07

837-839

The objective of these studies was to introduce the Stewart approach to analyses of acid-base changes in dogs with the chronic renal failure (CRF). The acid-base status was investigated in 12 healthy dogs and 20 dogs with CRF. In the CRF affected dogs the level of urea and creatinin rose to 350 mg/dl and 394.8 µmol/l respectively. The three independence variables (Pсо₂, SID-strog ion difference, and the Atot-sum of net charges of nonvolatile plasma buffers (albumin + inorganic phosphate) together with the strong ion gap (SIG) were calculated by a method adopted from articles employing Stewart’s approach. The SID averaged 41.85 mEq/l and 48.81 mEq/l in normal and diseased dogs respectively. Nonvolatile plasma buffers (Atot) were higher especially in more severely diseased dogs. The most considerable changes in affected dogs have been observed in SIG where, together with the increasing values of urea, the quantity of SIG successively dropped, attaining negative values (-28.03 mEq/l). The albumin values were significantly associated with the SID values. All affected dogs have metabolic acidosis partly compensated by respiratory alkalosis. As has been clearly observed the estimation of SIG may be used as an accurate marker of CRF in dogs.

Concentrations of carnosine, anserine, L-histidine and 3-methyl histidine in boar spermatozoa and sheep milk by a modified HPLC method

63%

Ducci M. , Pacchini S. , Niccolini A. , Gazzano A. , Cerri D. , Gadea J. , Bobowiec R. , Sighieri C. , Martelli F.

Polish Journal of Veterinary Sciences

2006

tom 09

nr 3

The present study deals with the application ofhigh-perform ancc-liquid-chromatographyl(HPLC) method for a quantitative detection of carnosine, anserine, L-histidine and 3-methyl-L-histidine in biological material with o-phthaldialdehyde (OPA) post column derivatisation at the constant temperature of 50°C. For this purpose, some mobile-phases were prepared with scalar acetonitrile concentrations. A complete separation of all molecules, particularly for carnosine and 3-methyl-L-his- tidine, was obtained with a solution of acetonitrile and 6mM hydrochloric acid with 0.48 M sodium chloride (5%:95% v/v). Post column derivatisation reaction at temperature of 50°C permitted to obtain an increase in sensibility of all molecules. This method has been utilised for detection of histidine dipeptides in boar spermatozoa and in sheep milk. Concentrations (mean ± S.E. nmol/109 spermatozoa) of carnosine (0.96 ± 0.14) and anserine (0.83 ± 0.18) in boar spermatozoa were significantly lower than those of L-histidine (52.85 ± 4.86) and 3-methyl-L-histidine (83.07 ± 7.1). Positive correlation was found between carnosine and anserine contents (r=0.740; p<0.01) and between L-histidine and 3-methyl-L-histidine (r=0.657; p<0.01). All histidine dipeptides studied were also present in 40 samples of sheep milk. In a case of samples without unit-forming colonies (UFC) of Staphylococcus coagulase-positive, carnosine concentrations (9.17 ±0.89 nmol/ml) were higher than anserine (0.51 ±0.02 nmol/ml) and both were significantly lower in respect to L-histidine (49.51 ± 6.48 nmol/ml) and 3-metyl-L-histidine (81.21 ±6.82 nmol/ml). A negative correlation was observed between carnosine milk levels (r=-0.773; p<0.01) and UFC/ml of Staphylococcus coagulase-positive. In conclusion this very simple and fast method can be used to detect histidine dipeptides in biological compartments where their concentrations are very low.