Wyniki wyszukiwania - Biblioteka Nauki

1

RAPD markers and black pine [Pinus nigra Arnold] intraspecies taxonomy - evidence from the study of nine populations

100%

Liber Z. , Nikolic T. , Mitic B. , Satovic Z.

Acta Societatis Botanicorum Poloniae

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2003

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tom 72

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nr 3

EN

Although intraspecies researches within the black pine (Pinus nigra Arnold) have a long tradition, the intraspecies taxonomy, classification and chorology are still unclear. Among the numerous reasons that have caused this situation the most important are: the absence of a study that would completely cover the whole range of this species, the impossibility of connection of results of the existing detailed studies of certain areas, and the high variability of traits which have been used so far. Since the characteristics of the molecular systematic techniques could make possible the research free of the mentioned shortages, the intention of this study was to determine the relationships among nine populations of black pine using the random amplified polymorphic DNA (RAPD). The obtained results were compared to the recent results of the morphological and anatomical analysis of the leaves of the same populations. The RAPD results clearly divided the Croatian populations from populations of Austria (subsp. nigra) and Turkey (subsp. pallasiana), while among Croatian populations, as in previous study, the existence of several groups (subsp. illyrica, subsp. dalmatica and transitional population between them) was noticed. It is assumed that the optimisations conducted in this study will finally make possible estimating the relationships on the level of the whole range of the black pine and the classification based on molecular traits that are probably less dependent on environmental influences than it has been the case with the characteristics mostly used so far.

2

Morphological and anatomical relationships in Alpine-Dinaric populations of the genus Iris L., Pallidae series [A.Kern.] Trinajstic [Iridaceae]

100%

Mitic B. , Nikolic T. , Liber Z.

Acta Societatis Botanicorum Poloniae

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2000

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tom 69

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nr 4

3

Morphological and karyological relationships within alpine-dinaric populations of the genus Iris L., Pallidae series [A.Kern.] Trinajstic [Iridaceae]

100%

Mitic B. , Nikolic T. , Liber Z.

Acta Societatis Botanicorum Poloniae

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2001

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tom 70

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nr 3

4

Plant DNA isolation from differently preserved Thalictrum leaf tissues and its use in RAPD analysis

100%

Liber Z. , Nikolic T. , Mitic B.

Acta Biologica Cracoviensia. Series Botanica

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2002

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tom 44

EN

There are many unsolved taxonomic problems at the intraspecific level in the genus Thalictrum (Ranunculaceae), which may be clarified using molecular systematics methods. No molecular systematics methods have been applied yet to the genus Thalictrum; this study analyzes different leaf tissue preservation and DNA isolation techniques, and the applicability of RAPD. A modified DNA isolation procedure using solution of laundry detergent as a detergent buffer system was the most suitable, especially for dealing with large samples. Since the use of differently preserved leaf tissues simultaneously with fresh leaf tissue may improve sampling in taxonomic research, and because the RAPD technique is sensitive to different factors, the possible drawbacks of using such tissues in RAPD analyses were checked. Of the four preserved leaf tissues, only DNA from silica gel-preserved leaf tissue gave suitably reliable RAPD results to be used with fresh leaf tissue in more extensive taxonomic research. Differently preserved leaf tissues are very problematic starting materials for simultaneous use with fresh leaf tissue in the same RAPD analysis. If differently preserved leaf tissues are to be used and reliable results are to be obtained, research techniques similar to those used in this paper should be applied.

5

Analysis and distributional patterns of the invasive flora in a protected mountain area - a case study of Medvednica Nature Park (Croatia)

75%

Vukovic N. , Bernardic A. , Nikolic T. , Hrsak V. , Plazibat M. , Jelaska S. D.

Acta Societatis Botanicorum Poloniae

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2010

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tom 79

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nr 4

EN

In this paper we have analysed invasive flora of Medvednica Nature Park, Croatia with respect to their origins, life forms, systematic positions, types of seed dispersal, Ellenberg indicator values and spatial distributions using MTB 1/64 grid units for analyses. A total of 27 invasive plant species, belonging to 14 families, were recorded with Asteraceae being the most frequently occurring family. Therophytes were the most common life form, as is generally true of Croatian invasive plants; however, hemicryptophytes and geophytes were more frequent in Medvednica. Here, invasive plants originated mainly from both Americas with slightly lower portion in comparison to all Croatian invasive plants, while contrary was the case when comparing those originated from Asia. The most widespread species was Erigeron annuus (L.) Pers., and the species with the lowest occurrence were Chamomilla suaveolens (Pursh.) Rydb and Datura innoxia Mill. A multiple regression model explains 44% of the spatial variability in the invasive plants data per MTB 1/64 unit, using the number of all recorded plant species, the average elevation and the lengths of paths and roads as estimators. The latter two variables also had the most influence on the ordination axes in analyses of the spatial distribution of seed dispersal types present in each MTB 1/64 unit. Anemochory was the most frequent type of seed dispersal.