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tom 44
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nr 2
EN
Anticoccidial drugs with antibiotic or antibacterial activity can be detected by microbiological agar diffusion methods based on the ability of certain drugs to inhibit the growth of sensitive strains of bacteria which act as test organisms and produce inhibition zones. In practice, microbiological agar diffusion methods form the basis of monitoring the residues of inhibitory substances and obtaining primary proof of the presence of residues in foods and raw materials of animal origin. The aim of the present study was to observe the sensitivity of individual test organisms to residual concentrations of selected types of anticoccidial drugs. In our study, four bacterial strains were tested by agar diffusion: Bacillus stearothermophilus var. calidolactis C 953, Bacillus subtillis BGA (at pH 6.0, pH 7.2 and pH 8.0), Micrococcus luteus ATCC 9341 and Bacillus cereus ATCC 11778. The following anticoccidial drug standards were used: amprolium, nicarbazin and furazolidone as chemical compounds, monensin and lasalocid as polyether ionophores, and sulfamethazine and sulfaquinoxaline as representatives of the sulfonamide group. The residual concentrations capable of inhibiting the growth of individual test organisms in our study were compared with maximum residual limits that have been established for these anticoccidial drugs. All anticoccidial drugs were tested using dilutions from 0.0001 mg/ml up to 1 mg/ml.
EN
Mycotoxins are secondary metabolites of fungi, which may cause diseases in animals or humans. Aflatoxin B₁ is mycotoxin, which is known to frequently contaminate poorly stored food products destinated for human consuption. In nature, there exist microorganisms for which the aflatoxins are non-toxic. Aflatoxins are degraded through the microorganism’s activity and the obtained products are probably utilized in their metabolism or the microorganisms have ability to bind aflatoxins to their surface. Components of herbs and spices have antiaflatoxigenic properties. They inhibit fungal development and subsequent aflatoxin production. The study reviews literature concerning the detoxification of mycotoxins by microorganisms and components of herbs and spices.
EN
The latest international crisis of the financial markets highlights the risks of instability, and the consequences of financial crime have a considerable effect on the performance of enterprises. The manipulation of selling prices or elements of financial statements even contribute to economic and social conflict in certain countries. The purpose of this study is to explore whether the conformity to Benford’s law of forensic accounting data is sustainable in the case of wholesale trade enterprises in the examined (2009-2015) period and Hungarian region. Our research aims to test various elementary and advanced (goodness-of-fit) statistical techniques to assess the validity of the reported statements. Overall, after examining the deviations in reflected and expected frequencies we assert the non-conformity of this law in some cases. However, our motivation is not only to suggest applied recommendations to enhance accountability for entrepreneurships, but also to outline further research directions to improve the forensic fraud detection and better understanding of accountancy crimes for the management.
PL
Ostatni międzynarodowy kryzys na rynkach finansowych podkreśla ryzyko niestabilności, a konsekwencje przestępstw finansowych mają znaczny wpływ na wyniki przedsiębiorstw. Manipulowanie cenami sprzedaży lub elementami sprawozdań finansowych przyczyniają się nawet do konfliktów gospodarczych i społecznych w niektórych krajach. Celem niniejszego artykułu jest zbadanie, czy dostosowanie do prawa danych księgowych Benforda jest trwałe w przypadku przedsiębiorstw handlu hurtowego w badanym okresie (2009-2015) w regionie Węgier. Przeprowadzone badania mają na celu przetestowanie różnych elementarnych i zaawansowanych (odpowiednio dopasowanych) technik statystycznych w celu oceny wiarygodności zgłaszanych oświadczeń. Po zbadaniu odchyleń w odzwierciedlanych i oczekiwanych częstotliwościach, w niektórych przypadkach stwierdzono niezgodność z tym prawem. Jednak motywacją autorów jest nie tylko zasugerowanie stosowanych zaleceń w celu zwiększenia odpowiedzialności w zakresie przedsiębiorczości, ale także nakreślenie dalszych kierunków badań w celu poprawy wykrywania oszustw i lepszego zrozumienia przestępstw związanych z rachunkowością zarządczą.
EN
The effects of Intellectual Property Rights (IPRs) is one of the economic mysteries of the current literature. However, at sectoral-level, it has still failed to provide any obvious empirical evidence regarding any direct link between patents and productivity. The chief contribution of this research paper is to provide empirical evidence on how chemistry patents relate to productivity growth in the case of a low-tech intensive industry (Food, Beverage and Tobacco). In order to support both the theoretical and empirical findings of the literature a dynamic regression model is tested and found a valid representation of the negative relationship between food chemistry patents granted and productivity growth in the long run. Subsequently, some conclusions and policy implications are suggested in order to support the enhanced productivity growth performance of these food industries, highlighting the importance of changes in current property protection systems.
PL
Skutki funkcjonowania Prawa Własności Intelektualnej (PWI) są ciągle jedną z tajemnic gospodarczych w obecnej literaturze. Jednak na poziomie sektorowym wciąż nie udało się dostarczyć żadnych oczywistych dowodów empirycznych dotyczących jakiegokolwiek bezpośredniego związku między patentami a produktywnością. Głównym wkładem tego artykułu badawczego jest dostarczenie empirycznych dowodów na to, w jaki sposób patenty dotyczące chemii wpływają na wzrost produktywności w przypadku przemysłu o niskim poziomie zaawansowania technologicznego (żywność, napoje i wyroby tytoniowe). Aby wesprzeć zarówno teoretyczne, jak i empiryczne ustalenia zawarte w literaturze, przetestowano model regresji dynamicznej i stwierdzono, że jest to prawidłowa reprezentacja negatywnego związku między przyznawanymi patentami na chemię spożywczą a wzrostem produktywności w długim okresie. Następnie sugeruje się wnioski i implikacje polityczne, aby wesprzeć zwiększony wzrost wydajności w gałęziach przemysłu spożywczego, podkreślając znaczenie zmian w obecnych systemach ochrony dóbr.
EN
The aim of this study was to use the natural pigment produced by Monascus purpureus as a substitute for nitrites in the production of meat products. Two different concentrations of a Monascus purpureus extract (0.5 g.kg⁻¹ and 0.75 g.kg⁻¹) were tested and compared with the control sample (C) containing a nitrite salting mixture without any addition of Monascus purpureus extract. Based upon the results, poultry ham prepared with half the quantity of nitrite salting mixture and 0.5 g.kg⁻¹ of Monascus purpureus extract showed the most desirable colour, flavour and appearance, the best microbiological parameters and the most suitable salt content.
EN
The sensitivity of three microbiological antibiotic residue screening methods - the Four Plate Test (FPT), the Screening Test for Antibiotic Residues (STAR) and the Premi®Test - was compared for the detection of 10 different standards from the sulphonamide (SA) group. Phtalylsulphathiazole (PHT), sulphadimidine (SD), sulphaguanidine (SG), sulphachlorpyridazine (SCHP), sulphamerazine (SRZ), sulphamethoxazole (SMX), sulphanilamid (SAM), sulphanilic acid (SAC), sulphaquinoxaline (SQ) and sulphathiazole (STZ) were tested using the concentrations from 0.05 µg.ml⁻¹ to 1 µg.ml⁻¹. The detection sensitivity of the methods represented by minimum inhibiting concentrations (MIC) of SA standards was evaluated. The MIC of SAs represent the detection limits of methods (LOD) for individual substances. The MIC of SA standards detected by the FPT was 0.2 µg.ml⁻¹ for SMX, STZ, SQ and SRZ, 0.3 µg.ml⁻¹ for SCHP, and 0.4 µg.ml⁻¹ for SD. The MIC of SA standards detected by the STAR was 0.05 µg.ml⁻¹ for SMX, STZ, SQ and SCHP, 0.1 µg.ml⁻¹ for SRZ, and 0.3 µg.ml⁻¹ for SD. No detection sensitivity of either method was observed for SAM, SAC, SG and PHT standards. The MIC of SA standards detected by Premi®Test was 0.05 µg.ml⁻¹ for SMX, STZ, SQ, SCHP, SRZ and SD, 0.1 µg.ml⁻¹ for PHT, 0.2 µg.ml⁻¹ for SAM, and 0.3 µg.ml⁻¹ for SAC and SG. The results of the tests showed that Premi®Test is the most sensitive method to detect Sas, followed by the STAR and the FPT methods. The inhibitory effect of para-aminobenzoic acid (PABA) toward the residual concentrations of SA standards was evaluated. The reference PABA concentrations for the confirmation of the presence of all SAs detected by the FPT and the STAR was 1 µg.ml⁻¹ and 10 µg.ml⁻¹ for the Premi®Test. In the authors’ opinion, the presented concentration of PABA can be recommended for a reliable confirmation of the presence of SA residues even at concentrations that present a potential risk to human health.
EN
The goal of the work was to monitor the occurrence of helmintologic disorders of dogs in the district of Košice-surroundings. Moreover, the authors wished to evaluate the potential devitalising effect of the following selected chemical substances and disinfective agents: NaOH (5%, 70°C), Savo (10%), Saniten (10%), H1(100%) on eggs of T. canis under laboratory conditions. Using coprologic examination the authors realized that the most common eggs in dog feces are of T. canis (41.4%). Then follow eggs of Trichuris sp. (21.8%), T. leonina (11.5%), eggs from the Ancylostomatidae group (9.2%) and Capillaria sp. (2.3%). Under laboratory conditions, the ovocide effectiveness of NaOH on nonembryonated eggs of T. canis by an exposition of 180 minutes was 23.98 ± 4.33%. The disinfective agent SAVO also had low effectiveness; by an exposition of 180 minutes it devitalized 24.77 ± 5.33% of eggs of T. canis. A higher effectiveness was evidenced by the disinfective agent Saniten. Using the disinfective agent H1, 98.04 ± 1.77% eggs of T. canis were devitalized. Based on the testing of each agent under laboratory conditions the authors can recommend the usage of the disinfective agent Hviezda, as it evidenced the best devitalizing effect on the eggs of T. canis.
EN
The study investigated the presence of sulphadimidine residues in eggs after per oral administration of Sulfadimidin PG plv. sol. ad us. vet. (120 mg/hen/day) to laying hens. Premi®Test, a four-plate microbiological method, and HPLC were used to detect sulphadimidine residues. The positive findings of the Four-plate test (FPT) were confirmed by the results of Premi®Test. The absence of sulphadimidine residues was confirmed 72 hours after the last sulphadimidine administration by using the FPT. The presence of sulphadimidine residues was detected by Premi®Test within 8 days and by the FPT within 3 days after the last administration. The FPT test reported false-negative results for five days (kappa < 0.6) as compared with the results of Premi®Test. The study confirmed the conformity of results obtained by both Premi®Test and HPLC (kappa = 0.6).
EN
The composition of mycoflora in storage rooms, and other rooms in a poultry-processing plant, as well as on the surfaces of egg shells was observed. The concentrations of both aflatoxin B₁ and ochratoxin A were determined in the shell eggs at room temperature and humidity, at a higher temperature and humidity, and in the eggs previously contaminated by Aspergillus flavus. We found that there was a reciprocal correlation between the presence of microscopic filamentous fungi in the air and on the working tables (Cladosporium spp. 45.5%, Penicillium spp. 36.4%, Mucor spp. 9.0%). The penetration of mycotoxins through the egg shell was relatively low and the residue limit of aflatoxin B₁ allowed (5 μg.kg⁻¹) was not exceeded in any sample of egg tested. However, the residue limit of ochratoxin A (20 μg.kg⁻¹) was exceeded in one case.
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