Wyniki wyszukiwania - Biblioteka Nauki

Nowa wersja platformy, zawierająca wyłącznie zasoby pełnotekstowe, jest już dostępna.
Przejdź na https://bibliotekanauki.pl

Ograniczanie wyników

Znaleziono wyników: 2

Liczba wyników na stronie

Wyniki wyszukiwania

Sortuj według:

Ogranicz wyniki do:

Effect of deletion mutation on the recombination activity of Cre recombinase

100%

Rongrong L. , Lixia W. , Zhongping L.

2005

tom 52

nr 2

Cre recombinase from bacteriophage P1 is widely used in both in vitro and in vivo DNA manipulations. Based on a structural and functional analysis, three deleted cre mutants were constructed and expressed in Escherichia coli. Mutated recombinases were purified and their recombination activities were determined in vitro. Our results revealed that the mutant with amino-terminal deletion retains the recombination activity as high as wild type Cre; however, the carboxy-terminal deletion and the middle region deletion both lead to a complete loss of the recombinase function.

Molecular detection and genetic diversity of porcine circovirus type 3 in commercial pig farms in Xinjiang province, China

51%

Mengfan Q. , Xifeng W. , Guowu Z. , Qingling M. , Jun Q. , Lixia W. , Kuojun C. , Jinsheng Z. , Zaichao Z. , Weiwei Y. , Yelong P. , Xuepeng C.

2019

tom 63

nr 4