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EN
Standard management of ovarian cancer includes cytoreductive surgery and subsequent adjuvant chemotherapy. The purpose of this study was an evaluation of the treatment process and toxicity of taxol and cisplatin in a standard medical procedure for women who underwent a surgery due to ovarian cancer. Participants of our study were 30 women, operated in our department due to ovarian carcinoma. The tolerance of chemotherapy was assessed according to WHO toxicity scale. The answer to the treatment in terms of remission was observed in 80% patients. The results of our study are able to have an important clinical value for future chemotherapy treatment.
EN
Objective: The aim of this study was to assess ovarian cancer angiogenesis by comparing intratumoral microvessel density (MVD) with pericytes expression. Methods: Tumor specimens from 34 women operated because of malignant adnexal tumors were evaluated. Pericytes were assessed with anti-desmin antibody and microvessels were counted following CD-34 antigen detection. Histological type, grading, FIGO stage and menopausal status were compared with MVD and pericytes expression. Results: The means of MVD in serous and mucinous tumors were 25 (range: 8-83) and 22 (range: 8-50), respectively. The difference between medians of MVD and mean number of pericytes per high power field were not statistically significant. Also, there was no correlation between assessed markers and differentiation grade and FIGO stage and tumor type. Conclusion: MVD counting with CD-34 antigen, but not desmin expression for pericytes detection seems to be a useful parameter for neoangiogenesis assessment in ovarian cancers.
EN
To compare Color Doppler sonography with immunohistochemical microvessel density and Bcl-2 assessement in women with benign and malignant adnexal tumors we examined 112 women with adnexal tumors with the use color Doppler sonography (B&K 2002 ADI and GE Voluson 730 scanners) performed before the surgical procedure. Histological type, grading, FIGO stage and menopausal status were compared with MVD and Bcl-2 expression assessed with immunohistochemistry on parafín embedded tumor specimens. Results: Sixty-seven tumors were benign and 45 tumors were malignant. The mean age of the studied women was 40,4 years; ±SD, 11,5 years. Color Doppler sonography revealed that in most malignant tumors there was a low resistance to flow (mean PI= 0,56, SD=0,36). Benign tumors also had higher pulsatility indices than malignant tumors (PI=1,21 and SD=0,48; p=0,02, Mann-Whitney U-test). Also, there was a statistically significant difference between MVD in benign and malignant tumors (Mann-Whitney U test, PO.OO 1). No significant difference in the percentage of Bcl-2 stained cells in both malignant and benign tumor samples was found. Conclusion: Microvessel density assessed with CD-34 and color Doppler blood flow assessement are useful in ovarian tumors vascularity studies. Bcl-2 expression is probably not a useful marker of angiogenic tumor character.
EN
The aim of the study was to determine the potential prognostic significance of intratumoral microvessel density assessment with panendothelial marker CD34, a new and specific marker for new blood microvessels CD 105, and a potent angiogenesis modulator, epidermal growth factor receptor (EGF-R) expression in women with serous ovarian cancer. Our results suggest that strong expression of EGF-R may be a prognostic of prognostic value in women operated because of serous cancer of ovaries.
EN
Objective: The biological effects of variable magnetic fields (MF) generated by widely used electrical devices on living organisms are not well understood. However MF may be potentially hazardous for pregnant women, this problem was not profoundly studied. The aim of this study was to evaluate the influence of the variable magnetic field (MF) on the placental metabolism by measurement of glucose consumption and lactate production in the human placenta under dual recirculating perfusion conditions. Material and methods: Altogether 20 term human placental cotyledons were exposed to homogeneous variable MF of B = 2 mT and f = 50 Hz (n=10; group E,) or of B = 5 mT and f = 50 Hz (n=10; group E2) during 3 hours dual close perfusion in vitro. 10 term placental cotyledons were perfused without an exposure to the MF and they served as a control group. Results: The results showed no effect of used MF on the glucose consumption either in group E, or in group E2in. There was also no influence of MF on lactic acid production in group Er In group E2, however, a significant increase of lactic acid production in the fetal circulation from 90lh to 180th minute and in the maternal circulation at 180 minute was noted. Conclusions: An increase in the lactic acid production in the group E2in observed during the experiment may result from hindered oxygen supply to the placenta and intensified anaerobic metabolism of glucose.
EN
The paper presents the results of morphological examinations of the human cotyledons perfused in vitro and exposed to variable magnetic field (MF) during the 180-minute experiment. The cotyledon biopsies were collected immediately after perfusion and morphologically examined using the electron microscope. The control group C (10 perfusions) was not exposed to MF. In the experimental group E, (10 perfusions), the cotyledons were exposed to the 2mT, 50Hz variable magnetic field while in the experimental group E2 (10 perfusions) the 5mT, 50 Hz was used. In the groups E, and E2, numerous indentations of the sheath (areola) in the syntrophoblast nuclei were found, condensed (thickened) nuclear chromatin right beneath the sheath was substantially reduced and pyknosis was observed in some nuclei. The villi revealed widened vascular - epithelial membrane resulting from the oedema of the endothelial cells. Moreover, an increased number of collagen fibres in the villi and decreased number of active mitochondria were observed in the group E2
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