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Heteroplasmy in plants

100%

Woloszynska M. , Oziemlak A. , Janska H.

Biotechnologia

2001

nr 1

42-46

Heteroplasmy is the state characterised by the presence of more than one type of mitochondrial or chloroplast genome in one organism. Infrequent recombinations across short repeated sequences often lead to heteroplasmy in higher plant mitochondria. Different Phaseolus species have been examined in order to understand the dynamics of heteroplasmy originating through recombination mediated by the 315 bp repeated sequence. Two techniques were applied to detect heteroplasmy: Souhern hybridization with prolonged autoradiography and PCR amplification followed by hybridization or reamplification. In all examined genomes the four recombination forms were detected. However, these forms do not occur in the same relative amounts. Moreover, in the examined genomes different recombination forms exist at the predominant/substoichiomeric level, but always only two of them are predominant. Based on these results and the previous data, we suggest that the changes in the heteroplasmic population of mitochondrial molecules in plants may offer the source of genetic variation in the course of evolution.

Screening of cytoplasmic DNA diversity between and within Lupinus mutabilis Sweet and Lupinus albus sensu lato by restriction fragment length polymorphism (RFLP)

88%

Olczak T. , Rurek M. , Janska H. , Augustyniak H. , Sawicka-Sienkiewicz E.

Journal of Applied Genetics

2001

tom 42

nr 2

127-137

Seven populations and five mutant lines of the Andean lupin and four species from the section Albus were screened for their mitochondrial and chloroplast polymorphisms. For this purpose the RFLP method with EcoRI as a restriction enzyme was used. Lupinus luteus, Lupinus albus and Phaseolus vulgaris organellar clones as well as amplified fragments were used as probes. We found that mitochondrial probes were more suitable than chloroplast probes for identification of inter- and intra-specific variations within the examined material. Most mitochondrial probes differentiate the two species investigated. A high level of mitochondrial polymorphism was observed among the populations of L. mutabilis in contrast to monomorphism among the species in the section Albus. A limited polymorphism was detected between the mutant lines of L. mutabilis. We conclude from this study that the mitochondrial RFLP analysis is a valuable tool for identification of variability among Andean lupin populations.