Wyniki wyszukiwania - Biblioteka Nauki

1

Evaluation of the relationship of the climbing level of sport climbers with selected anthropometric indicators and diet composition

100%

Przeliorz-Pyszczek A. , Golabek K. , Regulska-Ilow B.

Central European Journal of Sport Sciences and Medicine

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2019

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tom 28

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nr 4

2

Expression profiles of MGMT, p16, and APC genes in tumor and matching surgical margin from patients with oral squamous cell carcinoma

100%

Strzelczyk J. K. , Golabek K. , Krakowczyk L. , Owczarek A. J.

Acta Biochimica Polonica

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2016

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tom 63

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nr 3

3

Nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates

88%

Strzelczyk J. K. , Slemp-Migiel A. , Rother M. , Golabek K. , Wiczkowski A.

Acta Biochimica Polonica

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2013

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tom 60

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nr 4

EN

One of the mechanisms of Candida albicans resistance to azole drugs used in antifungal therapy relies on increased expression and presence of point mutations in the ERG11 gene that encodes sterol 14α demethylase (14DM), an enzyme which is the primary target for the azole class of antifungals. The aim of the study was to analyze nucleotide substitutions in the Candida albicans ERG11 gene of azole-susceptible and azole-resistant clinical isolates. The Candida albicans isolates represented a collection of 122 strains selected from 658 strains isolated from different biological materials. Samples were obtained from hospitalized patients. Fluconazole susceptibility was tested in vitro using a microdilution assay. Candida albicans strains used in this study consisted of two groups: 61 of the isolates were susceptible to azoles and the 61 were resistant to azoles. Four overlapping regions of the ERG11 gene of the isolates of Candida albicans strains were amplified and sequenced. The MSSCP (multitemperature single strand conformation polymorphism) method was performed to select Candida albicans samples presenting genetic differences in the ERG11 gene fragments for subsequent sequence analysis. Based on the sequencing results we managed to detect 19 substitutions of nucleotides in the ERG11 gene fragments. Sequencing revealed 4 different alterations: T495A, A530C, G622A and A945C leading to changes in the corresponding amino acid sequence: D116E, K128T, V159I and E266D. The single nucleotide changes in the ERG11 gene did not affect the sensitivity of Candida albicans strains, whereas multiple nucleotide substitutions in the ERG11 gene fragments indicated a possible relation with the increase in resistance to azole drugs.

4

Selected mechanisms of molecular resistance of Candida albicans to azole drugs

75%

Golabek K. , Strzelczyk J. K. , Owczarek A. , Cuber P. , Slemp-Migiel A. , Wiczkowski A.

Acta Biochimica Polonica

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2015

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tom 62

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nr 2

5

Assessment of nutritional status on the basis of the mini nutritional assessment (MNA) questionnaire in patients on the internal medicine ward

75%

Golabek K. , Mandecka M. , Chalaj A. , Drozdz K. , Szuba A. , Regulska-Ilow B.

Roczniki Państwowego Zakładu Higieny

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2018

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tom 69

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nr 3

EN

Background. The inadequate nutritional status of hospitalized patients leading to the malnutrition is one of the crucial clinical problems. Objective. The aim of the study was to assess nutritional status of patients on the basis of the mini nutritional assessment (MNA) questionnaire. Material and Methods. The study group included 120 patients staying on the internal medicine ward. The average age for female and male was 65±9.7 and 63±8.4 years, respectively. To assess malnutrition the anthropometric measurements and the MNA questionnaire was conducted. Results. The average MIS (Malnutrition Indicator Score) value in female and male was 25.0 ± 3.0. Among 29% of women and 18.2% of men the risk of malnutrition occurrence was noted. The incorrect values of BMI (body mass index) were observed more frequently in male compared to female (84.0% vs. 67.0%). Incorrect values of the MAC (mid arm circumference) and CC (calf circumference) were found respectively in 6.6 % and 10.5% of female and in 2.3% of male. 55.3% of women and 75% of men declared taking more than 3 prescription drugs a day. In women were observed a significantly higher deficiency of dairy products, meat, fish, or poultry as well as meals containing legume plants or eggs per week compared to men (21.0% vs. 4.5%). An insufficient daily intake of vegetables was declared by 20.4% of men and 15.8% of women. Conclusions. The occurrence of malnutrition or its risk was observed in about a quarter of studied hospitalized patients. The malnutrition was worsened by a reported loss of appetite, a significant number of drugs being taken, and inadequate nutrition among the patients.

PL

Wprowadzenie. Nieprawidłowy stan odżywienia, w populacji pacjentów hospitalizowanych, prowadzący do rozwoju niedożywienia, należy do istotnych problemów klinicznych. Cel badań. Celem badania była ocena stanu odżywienia pacjentów na podstawie ankiety MNA (Mini Nutritional Assessment). Materiały i Metody. Badaniem objęto 120 pacjentów hospitalizowanych na oddziale chorób wewnętrznych. Średnia wieku dla kobiet i mężczyzn wynosiła odpowiednio 65 ± 9,7 i 63 ± 8,4 lat. Aby ocenić stan odżywienia przeprowadzono badania antropometryczne i dokonano oceny stanu odżywienia na podstawie kwestionariusza MNA. Wyniki. Średnia wartość wskaźnika MIS (ang. Malnutrition indicator score) u kobiet i mężczyzn wynosiła 25,0 ± 3,0. Spośród 29% kobiet i 18,2% mężczyzn odnotowano ryzyko wystąpienia niedożywienia. Nieprawidłowe wartości BMI (body mass index) obserwowano częściej u mężczyzn niż u kobiet (84,0% w porównaniu z 67,0%). Nieprawidłowe wartości MAC (ang. Mid arm circumference) i CC ( ang. Calf circumference) stwierdzono odpowiednio u 6,6% i 10,5% kobiet oraz u 2,3% mężczyzn. 55,3% kobiet i 75% mężczyzn deklarowało przyjmowanie więcej niż 3 leków na receptę dziennie. U kobiet obserwowano znacznie większy niedobór produktów mlecznych, mięsa, ryb lub drobiu, a także posiłków zawierających rośliny strączkowe lub jaja w tygodniowej diecie, w porównaniu z mężczyznami (21,0% vs. 4,5%). Niewystarczające dzienne spożycie warzyw deklarowało 20,4% mężczyzn i 15,8% kobiet. Wnioski. Występowanie niedożywienia lub jego ryzyko obserwowano u około jednej czwartej pacjentów hospitalizowanych. Niedożywienie pogarszało się powodu zgłoszonej utraty apetytu, znacznej liczby przyjmowanych leków i nieprawidłowego odżywiania wśród pacjentów.

6

Selected CDKN2A and MDM2 polymorphisms in oral cavity cancer

63%

Gazdzicka J. , Golabek K. , Strzelczyk J. K. , Kiczmer P. , Miskiewicz-Orczyk K. , Krakowczyk L. , Ostrowska Z. , Misiolek M.

Acta Biochimica Polonica

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2020

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tom 67

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nr 2

7

Prevalence of Borrelia burgdorferi sensu lato in Ixodes ricinus ticks collected from Southern Poland

51%

Strzelczyk J. K. , Gazdzicka J. , Cuber P. , Asman M. , Trapp G. , Golabek K. , Zalewska-Ziob M. , Nowak-Chmura M. , Siuda K. , Wiczkowski A. , Solarz K.

Acta Parasitologica

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2015

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tom 60

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nr 4