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EN
In most higher plants, the female gametophyte is deeply embedded in the ovule. In an earlier work we isolated egg cells from maize ovule tissues and analyzed egg cell lysates by polyacrylamide gel electrophoresis and mass spectrometry-based proteomics technology, and identified the major protein components expressed in these cells. The identified proteins included three cytosolic enzymes for the glycolytic pathway (glyceraldehyde-3-phosphate dehydrogenase, 3-phosphoglycerate kinase, triosephosphate isomerase), two mitochondrial proteins (ATP synthase β-subunit and adenine nucleotide transporter), and annexin p35. Our data indicate that the plant egg cell is rich in an enzyme subset for energy metabolism. This article provides a short overview of major proteins in animal eggs, reports on major protein components of maize egg cells, and compares the major proteins between animal and plant eggs.
EN
Stages in the assembly of the egg in the monogenean skin parasite Entobdella soleae have been studied using a fast preservation technique for transmission electron microscopy. The first event is the release by the germarium of a fertilised oocyte, which travels to the ootype followed by many vitelline cells. There are two types of Mehlis' gland and the secretion from one of these (beta) is thought to promote the release of the vitelline droplets, which fuse peripherally in the distal tetrahedral chamber of the ootype to produce the eggshell. Initially, the zygote lodges in the distal corner of the chamber, perhaps held in place by cortical granule material, and prevents shell deposition in this corner. However, this is temporary, and when the zygote leaves the corner the opercular eggshell is laid down. The egg appendage is assembled in the proximal tubular part of the ootype and the adhesive droplets on the appendage are derived from the second (alpha) type of Mehlis' secretion.
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