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PL
Podjęto próbę sprawdzenia czy i w jakim stopniu podawanie antagonisty receptorów beta- -adrenergicznych wpłynie na dynamikę zmian leukocytów krwi indyków traktowanych ACTH. Indyki 6-tygodniowe (Big 6), przez 72 godziny otrzymywały z wodą do picia propranolol. Ponadto, dwukrotnie, w odstępach 24-godzinnych ptaki doświadczalne otrzymały iniekcję ACTH. Dla oceny dynamiki zmian w obrazie białokrwinkowym, czterokrotnie pobrano krew (w 12, 48, 54 i 72 godzinie doświadczenia). Kontrolę stanowiła krew od indyków z fermy, utrzymywanych w podobnych warunkach. Na podstawie dokonanej oceny wskaźnika heterofile/limfocyty (H/L), potwierdzono wcześniejsze sugestie co do dużej czułości oraz przydatności obrazu krwi w ocenie oddziaływania czynników naporu środowiska. Równocześnie wykazano, że podawany indykom propranolol łagodzi zmiany wywołane podaniem ACTH. Łagodzący wpływ propranolol wydaje się dotyczyć tylko wczesnych etapów odpowiedzi ustrojowej.
EN
An attempt was made to check whether and to what degree administration of beta adrenergic antagonist influences the dynamics of blood leukocytes of the turkeys treated with ACTH. The six weeks old turkeys (Big-6) received propranolol in drinking water for 72 hours. Moreover, the birds received the ACTH injection twice, in 24 hours intervals. To evaluate the changes in the white blood cell picture, the blood was taken four times (in the 12th, 48th, 54th and 72nd hour of experiment). The control was the blood from the turkeys from poultry farm, which were kept in similar conditions. On the basis of estimation of the heterophils/lymphocytes (H/L) ratio, the earlier suggestions were confirmed, concerning the high sensitiveness and usability of blood picture in evaluation of environmental factors influence. Simultaneously, there was shown that the administered propranolol attenuates the changes induced by ACTH injection. The attenuating influence of propranolol seems to apply only to early stages of organism response.An attempt was made to check whether and to what degree administration of beta adrenergic antagonist influences the dynamics of blood leukocytes of the turkeys treated with ACTH. The six weeks old turkeys (Big-6) received propranolol in drinking water for 72 hours. Moreover, the birds received the ACTH injection twice, in 24 hours intervals. To evaluate the changes in the white blood cell picture, the blood was taken four times (in the 12th, 48th, 54th and 72nd hour of experiment). The control was the blood from the turkeys from poultry farm, which were kept in similar conditions. On the basis of estimation of the heterophils/lymphocytes (H/L) ratio, the earlier suggestions were confirmed, concerning the high sensitiveness and usability of blood picture in evaluation of environmental factors influence. Simultaneously, there was shown that the administered propranolol attenuates the changes induced by ACTH injection. The attenuating influence of propranolol seems to apply only to early stages of organism response.
EN
In the recent period, the number of cases of chronic form of liver fluke (Fasciola hepatica) in domestic ruminants has increased. The fluke is the most frequently recorded parasitic disease in bison. The diagnosis of the infection is based on hematological and biochemical blood tests, as well as on serological and coprological examinations. The aim of the study was to compare hematological and biochemical changes that occur in bison living in their natural environment, as well as in dairy cattle and sheep diagnosed with a chronic form of liver fluke. Material and methods: The research included 10 bison, 10 dairy cows and 10 sheep, in which a chronic form of liver fluke was diagnosed by blood tests as well as by coprological and anatomopathological examinations. Blood samples were collected after slaughter (bison) or before treatment (domestic ruminants). A hematological analyzer Horiba was used to determine the following blood parameters: WBC, RBC, PCV, Hb, MCV, MCH, MCHC, platelets, neutrophils, lymphocytes, eosinophils, basophils, and monocytes. A biochemical analyzer Mindray BS-130 was used to determine the concentrations of urea, total cholesterol (Chol), total protein (TP), albumin (ALB), creatinine (CREA), total calcium (tCa), magnesium (Mg), phosphorus (Pn), and ferrum (Fe), as well as the activities of AST, GGTP, and CPK. The concentration of globulins was calculated as the difference between the concentrations of TP and ALB. Results: In bison with a chronic form of liver fluke, hematological examination showed no evidence of changes. In cattle and sheep, the tests revealed normocytic anemia. Some cases of eosinophilia were found among bison and domestic ruminants. In all animals, AST and GGTP were increased. In domestic ruminants, the examination showed a reduction in creatinine and total protein, whereas in sheep, CPK and urea concentration were increased, and albumin was decreased. The content of iron in the blood of domestic ruminants was reduced or normal.
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