The bacteria from different phylogenetic groups were studied in surface microlayer (SM, up to 100 [mu]m ) versus subsurface water (SW - 20 cm) in eutrophic lake from spring to autumn of 2007. Abundance of bacteria was determined using a combination of direct counting of 4', 6-diamidino-2-phenylindole and the phylogenetic diversity was determined in fluorescence in situ hybridization (FISH) method with group-specific, fluorescently labeled oligonucleotide probes. The numbers of DAPI bacteria varied between 4.75 and fluorescence in situ hybridization (FISH) demonstrated that Eubacteria constituted the majority of the whole bacterial population and their percentage share ranged from 59 to 75%. Abundances of alpha- beta-Proteobacteria and Cytophaga-Flavobacteria groups varied across seasons, layers, and lacustrine zones. The lowest number of alpha-Proteobacteria group bacteria was observed in spring (SM - 0.2 x 10[^6], SW - 0.16 x 10[^6] cells cm[^-3]), whereas the highest in autumn (SM - 0.62 x 10[^6], SW - 1.6 x 10[^6] cells cm[^-3]). The percentage share of these groups of bacteria in the Eubacteria domain was lower in spring (20.50%) than in summer and autumn (from 65 to over 80%). No fixed difference between the composition of SM and SW bacteria was noticed. Seasonally occurred changes are similar in both layers.
Effects of post−harvest wood−debris utilization and pre−planting soil preparation in clear−cut forest on the community structure of soil fungi and bacteria and their possible biological activity towards Armillaria and Heterobasidion were studied in 1− and 10−year−old Scots pine plantations in Bierzwnik and Międzychód Forest Districts (W Poland). Post−harvest wood−debris utilization included: (i) removal from the surface, (ii) spread of the coarse or chipped wood−debris on the surface and (iii) mixing of the chipped wood debris with the soil. Pre−planting soil preparation included: (i) deep furrowing, (ii) shallow turning of the topsoil, (iii) ridging and (iv) no ground preparation. The soil−dilution method was used for detection of fungi and bacteria in soil. Morphotyping was used for identification of fungi. Phenotypic traits and biochemical properties were used for identification of bacteria. Molecular method, MID−66 or BIOLOG® systems were additionally applied for identification of the most common bacteria. Removal of post−harvest wood−debris from the surface of the clear−cut land and shallow turning of the topsoil or ridging before planting increased abundance of fungi in soil of 1−year−old Scots pine plantation. Deep furrowing resulted in increased abundance of fungi and no ground preparation in increased abundance of bacteria in soil of 10−year−old Scots pine plantation. Increased abundance of fungi and bacteria was associated with increased abundance of taxa considered as antagonistic to Armillaria and Heterobasidion. Removal of the post−harvest wood debris and moderate or no mechanical intervention into the soil habitat on the clear−cut site before planting of Scots pine seedlings seems to create the habitat, which may be beneficial for the growth of young trees.