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1 Bromatologia i Chemia Toksykologiczna

1 1995

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Zanikanie 3H witaminy D3 z surowicy szczurow narazonych na dzialanie tiuramu

100%

Witkowska D. , Sedrowicz L.

nr 4

323-329

Zanikanie 3H witaminy D3 w surowicy krwi badano na szczurach samcach szczepu Wistar narażonych na działanie tiuramu przez 2 tygodnie. W oparciu o otwarty model dwukompartmentowy wyznaczono podstawowe parametry farmakokinetyczne, tj. biologiczny okres póltrwania – t0,5, stałe szybkości dystrybucji (k12 i k21) oraz stalą eliminacji (k13).

Male Wistar rats were used to test the effect of 14-day exposure to thiram on 3H vitamin D3 depletion from blood serum. Prior to the experiment, the method (Shepard et al.) of extraction and isolation of vitamin D3 and its metabolites (column chromatography) has been checked and suitably modified. Thiram suspended in soybean oil was administered to rats by stomach intubation at 5% LD50 for 14 days. The controls received similar volume of the oil. After 14 days, the control and thiram-treated rats were injected s.c. with 1 µCi 3H vitamin D3/100 g b.w. The animals were sacrificed 0.5, 1, 2,4, 8, 24 and 48 hours after injection. Blood samples were collected and centrifuged. Each serum sample (0.5 cm3) was extracted with methylene chloride and chromatographed on a column filled with Sephadex LH 20 and porous glass (1:1) to separate 3H vitamin D3 from its metabolites. A hexane- chloroform-methanol 9:1,5:1 mixture was used for elution. Suitable fractions were collected and sample radioactivity was determined using a LKB liquid scintillation spectrometer. The results were expressed in terms of 3H vitamin D3 dpm/1 cm3 serum. The open 2-compartmental model was used in the analysis of the results. Half-life time t, transfer rate constants k13, k21 and the constant of elimination k13 were determined. It has been demonstrated that intoxication with thiram leads to disorders in 3H vitamin D3 elimination from serum. It is reasonable to suppose that thiram may affect vitamin D3 metabolism.