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PL
Celem badania była ocena częstości występowania i wpływu zespołu objawów określanego jako bacterial vaginosis (BV) oraz zakażeń bakteryjnych dróg rodnych kobiet we wczesnej ciąży na ryzyko wystąpienia porodu przedwczesnego. BV stwierdzono u 51 (28,5%) ciężarnych. Odnotowano istotny wzrost odsetka porodów przedwczesnych w grupie kobiet z BV do 15,7% w porównaniu do grupy kobiet z prawidłową florą (9,1%). Spośród kobiet, które urodziły przedwcześnie u 38,1% stwierdzono BV, podczas gdy u kobiet rodzących po 37 tygodniu ciąży odsetek ten wyniósł 27,2% (OR=l,86). Infekcja C. trachomatis we wczesnej ciąży nie wiązała się ze wzrostem ryzyka wystąpienia porodu przedwczesnego.
EN
The main aim of this prospective study was to determine the prevalence and an association between pathological microflora of the lower genital tract diagnosed at early pregnancy and the risk of preterm delivery. The study group comprised 179 randomly selected pregnant women from Lodz region, between 8 and 16 week of pregnancy. For the qualitative and quantitative assessment of biocenosis of the lower genital tract vaginal and cervical swabs were collected from the pregnant women under study. The C. trachomatis antigen was detected by direct immunofluorescence assay. The vaginal swabs were tested for aerobic and anaerobic bacteria. Bacterial vaginosis was diagnosed by Gram stain according to Spiegel's criteria. To evaluate the risk factors odds ratios were calculated using EPI INFO software. 21 (11,7%) women delivered before 37"1 week of pregnancy. Bacterial vaginosis was diagnosed among 51 ( 28,5%) pregnant women while intermediate microflora was diagnosed by Gram stain in 62 (34,6%) women. The shortest mean gestational age at delivery was noted among women with BV The rate of preterm delivery in BV group was 15,7% comparing to 9,1% among women with normal microflora. Among women with preterm delivery BV was diagnosed in 38,1% (OR=1,86). Based on culture results only 84 (46,9%) women had normal microflora at early pregnancy. The pathological culture was associated with slightly increased preterm delivery rate (12,6%) as compare to 10,7% in control group. Positive culture for Bacteroides and Mobiluncus was connected with nonstatistical rise in the risk of preterm delivery. No association between C. trachomatis infection at early pregnancy and elevated risk of preterm delivery was found. Early pregnancy diagnosis of bacterial vaginosis and its treatment should lower the rate of prematurity in Poland.
EN
Pregnant gilts (in the first month of pregnancy) were infected intranasally with SIV H1N2. Clinical signs, presence of SIV RNA in nasal swabs, haemagglutination titer, hematological parameters, concentrations of interleukin (IL-10, IFNγ) and acute phase proteins (APP) (CRP, Hp), as well as production parameters, were evaluated. The concentrations of APP and cytokines were analyzed with the use of ELISA assays. Antibodies against SIV were detected with the haemagglutination inhibition assay, and the presence of RNA with RT-PCR. No clinical sings typical of swine influenza were found in the infected gilts. The gilts did not show any signs of pregnancy pathology, and no abortions were observed. No significant differences were found between the infected and control gilts with regard to the number of piglets born, hematological parameters, or the concentrations of APP and cytokines. The presence of specific antibodies against SIV was confirmed in the infected gilts starting from 14 dpi. The presence of SIV RNA in nasal swabs collected from the gilts was confirmed within 4 dpi. No SIV RNA were found in samples taken from newborn piglets.
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