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EN
The aim of this study was to assess the course of some parameters of non-specific immunity during the estrus cycle in bitches. Blood samples were collected from 28 healthy bitches at different stages (anestrus, proestrus, estrus, metestrus) of the estrus cycle. Nitroblue tetrazolium (NBT) reduction by blood neutrophils, phagocytic activity of neutrophils, proliferative response of blood lymphocytes to phytohemagglutynin, serum activity of lysozyme and ceruloplasmin, level of total protein and γ-globulin fraction were determined. The study showed that nitroblue tetrazolium (NBT) reduction by blood neutrophils, proliferative response of blood lymphocytes to phytohemagglutynin, serum activity of lysozyme and ceruloplasmin were significantly higher (p ≤ 0.01) in proestrus and estrus than in anestrus.
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tom 62
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nr 07
830-833
EN
The aim of the study was to compare KLP-602 or M. chelonae on some biochemical and nonspecific immunity parameters in turkeys immunized with the virus antigen. Research was carried out on 30 turkeys (3 groups, 10 birds in each), who were given one of the two immunity stimulators: KLP-602 or M. chelonae at the age of 29 days. The control group was comprised of birds receiving no stimulators. All the birds received an active mesogenic Roakin strain of ND virus after 7 days and again after 100 days and the biochemical parameters (total proteins, gammaglobulin, ceruloplasmin level), humoral defense parameters (level of lysozyme), cellular parameters (potential killing activity, respiratory burst activity and proliferation activity) were determined. The obtained results demonstrated that M. chelonae had a more significant stimulatory effect on biochemical and humeral immunity parameters than KLP-602.
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tom 62
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nr 11
1256-1260
EN
The aim of the present study was to compare selected biochemical and non-specific immunity parameters in turkeys vaccinated with Dindoral-SPF in response to a live virus antigen, the Roakin strain of Newcastle disease virus (NDV), after the administration of two synthetic immunity stimulators: isoprinozine and levamisol. The experiment was performed on 30 turkeys. At 24-days-of-age they were vaccinated with the commercial vaccine Dindoral-SPF containing an attenuated strain of hemorrhagic enteritis virus (HEV). At 29-days-of-age the turkeys were divided into three groups of ten birds each. Particular subgroups were given a single dose of either levamisol or isoprinozine, and the third group (the control) received none of the immunity stimulators. After 7 days and again after 100 days all birds received a live mesogenic Roakin strain of NDV. Biochemical and non-specific immunity parameters were determined in blood collected from the wing vein. The results obtained indicated short-term but statistically significant decreases and increases in the levels of biochemical parameters (total protein, gammaglobulins and ceruloplasmin) and non-specific immunity indicators (lysozyme activity) in both experimental groups, as compared with the control group. More interesting, indicating higher effectiveness of isoprinozine than levamisol, were the results concerning non-specific cellular immunity in turkeys, including a higher metabolic activity of leukocytes in the NBT test, especially during the primary response, and higher leukocyte reactivity in the TB test, reflected by RI values, especially during the secondary response. The phagocytic activity of leukocytes, expressed as a percentage of phagocytizing cells and the values of the phagocytic index, was the highest in isoprinozine-stimulated turkeys
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tom 63
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nr 05
557-560
EN
Ovariohysterectomy is the treatment of choice for pyometra and a common contraceptive method for bitches. The effectiveness of preventing the postoperative complications depends largely on the proper monitoring of the health condition of bitches. The non-specific immunity parameters in the postoperative period are evaluated using the NBT test as well as determining the phagocytic index, percentage of phagocytizing cells, lysozyme activity, serum levels of total protein and its globulin fraction. Our study shows that the values of selected parameters of non-specific immunity provide valuable information concerning the end of inflammatory reaction caused by the discontinuity of tissues in the normal postoperative course. Moreover, the data obtained indicate that such parameters may be used in routine diagnostic procedures of early postoperative complications.
EN
The aim of the study was to determine the effect of water extracts of fresh garlic, added to drinking water, on the immunological indices of blood, production results and chemical composition of meat. The experiment was performed on 204 heavy-type turkeys BIG 6 (male), reared until 18 weeks of age. One-day-old poults were divided at random into two groups, with 34 birds in each (3 replicates). All birds were fed on the same complete feed within the five-step system. Birds were had access to the experimental diets and water ad libitum. Drinking water was given in the control group without additives, but in the experimental group a water extract of crushed garlic was given twice a week at 0.5 g/l of water. The water extract of fresh garlic positively affected some blood parameters. This was confirmed by an increased lysozyme activity (by 7-45%), higher levels of leukocyte spontaneous migration (%), and a decrease in non-phagocyte cells (18 vs. 25%) as compared with the control group. Simultaneously, there was an increase in RBC (red blood cells), and Hb (hemoglobin) amount (by 8 and 20%, respectively), a lower cholesterol level, and a more favorable Ca/P ratio was noted. Turkeys given drinking water with water extract of fresh garlic were characterized by lower mortality (by 50%), decreased feed conversion rate (by 3.1%), and higher by 1.3% body weight as compared with the control group. Adding the water extract of fresh garlic to drinking water also affected the chemical composition of breast muscles, i.e. content of crude protein was higher by 3% (P £ 0.01), and fat and crude ash by 8%, respectively.
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