This paper aims at finding a simple method of determining a parameter, linking the deformation energy with impact velocity and extent (size) of damage, known as the resistance factor, and investigates its variation over the periods of harvest and storage. The resistance factor was found to be significantly influenced by the apple variety, date of the test and by method of storage. Apples of greater firmness were found to have higher values of resistance factor during harvest, this is valid for apple variety and the date of test. The resistance factor was found to undergo apparent rise during storage, probably as a result of the rise in viscosity and damping characteristics of the material.
PL
Celem pracy było opracowanie prostej metody określania parametru zwanego wskaźnikiem odporności, łączącego energię odkształcenia, prędkość uderzenia oraz wielkość uszkodzenia. Wykonano stanowisko do badań udarowych. Masę wahadła i kąt uderzenia ustalono doświadczalnie, oddzielnie dla okresu zbioru (m = 0.445 kg, α = 70°) i przechowywania (m = 0.445 kg, α = 50°). W doświadczeniu zastosowano typowy wgłębnik dla prób przebijania o średnicy 7/16 cala. Stwierdzono istotny wpływ odmiany, terminu badań i sposobu przechowywania na wskaźnik odporności. W okresie zbioru u jabłek o wyższej jędrności miąższu, wystąpiły większe wartości wskaźnika, dotyczy to odmiany i terminu badań. W okresie przechowywania stwierdzono pozorny wzrost wskaźnika, będący prawdopodobnie wynikiem wzrostu własności lepkościowych i tłumiących materiału.
The examinations were carried out on 40 healthy rabbits of mixed breed and sex weighting from 1.8 to 2.2 kg at the age of 12 weeks, i.e. at the beginning of the experiment, and 3.0 to 3.2 kg (20 weeks old) at the end of the examinations. The blood samples were taken 9 times at intervals of 7 days from the peripheral ear venas of rabbits, after which the ability of blood elements for adherence and phagocytosis was determined; moreover, the spontaneous and stimulated NBT tests were done. In addition the activity of myeloperoxidase in the blood cells, serum lysozyme activity, the level of IgG and total immunoglobulins expressed in ZST units were evaluated. The examinations revealed an increased tendency of adherence, nitrotetrazole reduction, the production of serum IgG, phagocytosis, and a drop in the amount and activity of serum lysozyme. No changes were observed regarding the myeloperoxidase activity, the percentage of phagocitizing cells and the amount of serum immunoglobulins calculated in ZST units. It was found that only the index of phagocytosis and spontaneous NBT test reduction were in accordance with data presented by other authors. The findings in respects to the percentage of phagocitizing cells differed significantly.
The aim of the study was to monitor alterations in selected indices of innate immunity (the phagocytic index and percent of phagocytes) and acquired immunity (numbers of lymphocytes T and their Th and Tc/Ts subpopulations) in 60 rabbits experimentally infected with haemagglutinogenic Czech strain CAMP V-351 and non-haemagglutinating Polish BLA. The haemagglutinogenic Czech strain CAMP V-351 is active only in select indices of innate immunity, whereas non-haemagglutinating Polish strain BLA is reactive in the parameters of acquired immunity. Therefore, the obtained results permit the conclusion that the examined strains are not only serologically different but they also induce distinct patterns of immune responses, which provides evidence of immunotypes among this virus.
The examinations were done on 7 healthy Polish ponies from 1.5 to 14 years of age. The experimental animals were intravenously injected Escherichia coli LPS at a dose of 0.1 µg/kg b.w. Clinical observations and blood collection for laboratory examinations from the external neck vein were done just before endotoxin injection and then for 8 hours at one hour intervals and after 24, 48, 72 and 96 hr. from endotoxin application. All horses were examined routinely clinically (rectal temperature, pulse, respiration rate) and hematologically (total number of leukocytes, differential leukocyte count). Phagocytic activity of neutrophilic granulocytes was evaluated on the basis of the percentage of phagocytic cells (KF) and the phagocytic index (IF) using a standard Staphylococcus aureus strain. Moreover, the nitrotetrazolium blue reduction test (NBT) was done by a microquantity method. Clinical observations and hematological examinations in the experimental animals were typical for endotoxemia of horses. It was also found that LPS of E. coli at the administered dose stimulates unspecific immunity because it increases the value of KF, IF and NBT during the whole period of experimentation.
The studies were carried out on 8 young pigs (30—45 kg of body weight) in order to assess the level of adrenalin, noradrenalin and cortisol in the plasma of animals forced to movement three times a day for 15 min at intervals of 45 min. The experiment lasted for 28 days. The blood was collected at day 1, 3, 7, 14 and 28 from the anterior intracranial vein. The indices of cellular immunity, i.e. the activity of neutral leukocytes (NBT) and the index of lymphocyte transformation were evaluated. It was found that prolonged intermittent physical stress brought about in young pigs a progressive decrease of the hormones and the immunological activity of leukocytes. No correlation between the level of cortisol and catecholamines was found.
Adult chickens (1.4—1.8 kg of body weight) were immobilized for 5 hours for 3 consecutive days after the prior application of immunosuppresive doses of levamisole for 14 days and immunostimulative injections of TFX (extract from thymus); the drugs were given alone or together. The concentration of corticosterone lysozyme, globulins, the degree of blast transformation and metabolic activity of leukocytes (NBT) were evaluated. The studies did not reveal any correlation between the level of corticosterone and the indices of immunity. An increase of corticosterone and its decrease was found following immobilization and the use of TFX and levamisole respectively. However, immobilization, TFX and levamisole brought about the growth of metabolic activity of leukocytes and lymphocytes transformation. The level of lysozyme was reduced by immobilization and the administration of levamisole; its growth was noted following the application of levamisole with immobilization at the same time. The above treatment of the animals diminished the level of globulins in the blood. The results indicated to a diversity of immune response in chickens and the lack of any correlation between it and the suprarenal gland.