Wyniki wyszukiwania - Biblioteka Nauki

1

Ocena wrazliwosci na antybiotyki wybranych szczepow bakteryjnych wyizolowanych z mleka zbiorczego

100%

Popiolek-Pyrz M.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

|

2004

|

tom 22

87-91

2

Wrażliwość krętków Borrelia burgdorferi sensu lato na antybiotyki in vitro

100%

Chmielewski T. , Tylewska-Wierzbanowska S.

Postępy Mikrobiologii

|

2017

|

tom 56

|

nr 3

3

Drobnoustroje wyizolowane z mleka cwiartkowego krow i ich wrazliwosc na antybiotyki

100%

Borkowska D. , Polski R. , Janus E.

Annales Universitatis Mariae Curie-Skłodowska. Sectio EE: Zootechnica

|

2006

|

tom 24

27-32

4

Wrazliwosc na antybiotyki szczepow Staphylococcus aureus

100%

Kruszynska E. , Bialek M. , Janicka G. , Bugalski R. , Belzyt E. , Czajkowski H. , Harenska K. , Wojciechowska D.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 3-4

141-144

EN

Staphylococcus aureus is a very important pathogen in humans about 20%of all bacterial infections are caused by S. aureus. Because the staphylococcal sensitivity patterns have changed, the aim of this study was to investigate the current susceptibility of the S. aureus strains to 9 antibiotics: penicilin (P), amoxacillin / clavulanic acid (AMC), erythromycin (E), cortimoxazole (SXT), tetracycline (T), chloramphenicol (C), mupirocin (MUP), gentamicin (Ge) and vancomycin (Va). Susceptibility testing was performed by disc diffusion technique, by the procedure outlined by the National Committe for Clinical Laboratory Standards (NCCLS). Susceptibility to methicillin was determined using 5 µm methicillin disks, ß-lactamase production in the penicillin - resistant strains was detected with nitrocefin impregnated disk (Cefinase, BBL Microbiology system). The microbiological characteristics of the samples: 338 of the isolates were derived, from noses and the throaths 382 - from surgical wound secretions. The analysis of the results showed that most S. aureus strains (82,8%) are penicillin resistant and ß-lactamase producing 13,5% of all strains were methicillin - resistant. 16,5% - were erythromycin resistant; 3,2% - were cotrimoxazolc resistant; 51,4% - were mupirocin resistant; 20,6% - were gentamicin resistant. All of the strains were vancomycin sensitive.

5

Wrazliwosc na wybrane tetracykliny paleczek z rodziny Enterobacteriaceae

100%

Sekowska A. , Ibsz-Fijalkowska A. , Goldyn K. , Gospodarek E.

Medycyna Doświadczalna i Mikrobiologia

|

2009

|

tom 61

|

nr 4

321-326

6

Ocena antybiotykowrazliwosci krajowych izolatow Streptococcus suis

88%

Szczotka A. , Markowska-Daniel I. , Pejsak Z.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 09

1077-1080

EN

An increasing number of S. suis strains showing resistance to commonly used antibiotics have been identified over the last years. The aim of the study was to determine the antibiotic susceptibility of Polish field S. suis strains using the broth microdilution method. The following antibiotics were included in the test: penicillin, ampicillin, ceftiofur, gentamicin, neomycin, spectinomycin, erythromycin, tylosin, tiamulin, chlotretracycline, oxytetracycline, enrofloxacin, sulphadimetoxin, sulphachloropyridazine, sulphathiazole, sulphamethoxazole with trimethoprim and florphenicol. The S. suis strains tested were collected between 1996-2005 from pigs indicating nervous symptoms, respiratory tract disorders and/or arthritis. The necropsy hyperaemia of meninges revealed inflammation of lungs and joints and the presence of serofibrinous exudate on the pericardium. The results of the study demonstrate the high level of susceptibility of tested strains to b-lactams (about 100% of susceptible strains), florphenicol (98%), gentamycin and a combination of sulphamethoxazole and trimethoprim (95%), enrofloxacin and spectinomycin (90.37%). A large percentage of strains were resistant to chlortetracycline (49%), oxytetracycline (40%), erytromycin and tylosin (50%) and to sulphonamides (50.37%). Most strains were susceptible to the lowest concentrations of b-lactams and sulphametoxazole with trimethoprim. The greatest antimicrobial activity was obtained for florphenicol, enrofloxacin, spectinomycin and gentamicin when the highest concentration of antibiotics was used in the study. The presented study confirms the high susceptibility of Polish field S. suis strains to the antibiotics recommended for treating streptococci. Positive therapeutic effects should be expected after using b-lactams, florphenicol and sulphonamides with trimethoprim, gentamicin and fluoroquinolones.

7

Wrazliwosc klinicznych szczepow bakterii Gram-dodatnich na wybrane antybiotyki beta-laktamowe

88%

Sawicka-Grzelak A. , Rokosz A.

Medycyna Doświadczalna i Mikrobiologia

|

1998

|

tom 50

|

nr 1-2

69-76

PL

W ciągu 4 miesięcy badań wyhodowano 111 szczepów bakterii Gram-dodat- nich od pacjentów z oddziału chirurgii ogólnej. Wśród wyizolowanych drob¬noustrojów dominowały gronkowce (49 szczepów) i enterokoki (44 szczepy). Wykryto 33 szczepy metycylinoopornych gronkowców. Penicyliny z inhibi¬torami ß-laktamaz oraz karbapenemy wykazały wysoką aktywność in vitro wobec klinicznych szczepów bakterii Gram-dodatnich z wyjątkiem E. fae- cium.

EN

The aim of the study was to determine the activity of four ß-lactam antibiotics against nosocomial strains of Gram-positive bacteria. Two antibiotics combined with ß-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in surgical ward of the National Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 STAPH, API STREP, API CORYNE and API 20 A strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB STAPH, ATB STREP and ATB ANA strips. The susceptibility of strains to timentin, tazocin, imipenem and meropenem was tested with disc diffusion method. 111 strains of Gram-positive bacteria were cultured. Staphylococci (49) and enterococci (44) dominated among isolated strains. 33 Staphylococcus spp. strains were identified as methicillin-resistant. The obtained results indicate a significant role of Gram-positive cocci (staphylococci and enterococci) in the aetiology of nosocomial infections. Antibiotics combined with ß-lactamase inhibitors and carbapenems demonstrate broad antibacterial spectrum against clinical strains of Gram-positive bacteria except E. faecium strains.

8

Wystepowanie i wrazliwosc na antybiotyki warunkowo chorobotworczych bakterii izolowanych z nasienia ogierow

88%

Danek J. , Wisniewski E. , Krumrych W. , Dabrowska J.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 08

385-387

EN

295 ejaculates of 150 stallions of various breeds (full English, Great Polish, Hannower and Polish ponny) used for mating in 17 horse breeding centres in Poland were examined bacteriologically. More than 23% of ejaculates were contaminated with facultative pathogenic bacteria, mostly by Pseudomonas aeruginosa (10% of samples) sensitive to streptomycin (100%) and neomycin (8%). Sir. zooepidemicus was also isolated (9.5%), sensitive mostly to chloramphenicol (73%), penicillin (64%) and oxytetra- cycline (50% of strains).

9

Wrazliwosc na wybrane antybiotyki beta-laktamowe klinicznych szczepow Escherichia coli i Klebsiella pneumoniae

88%

Andrzejewska E. , Szkaradkiewicz A. , Kaniasty M.

Medycyna Doświadczalna i Mikrobiologia

|

1998

|

tom 50

|

nr 3-4

197-205

PL

W pracy dokonano analizy lekooporności szczepów Escherichia coli i Klebsiella pneumoniae izolowanych od pacjentów. Wykonano testy określające zdolność bakterii do produkcji ß-laktamaz o rozszerzonym spektrum substratowym (ESBL).

EN

The studies aimed at analysing the resistance to some ß-lactam antibiotics among E. coli and K. pneumoniae clinical isolates and at evaluating. The extended spectrum of ß-lactamases (ESBL) production in the isolates. The analysis included 137 E. coli strains and 52 K. pneumoniae strains, isolated from hospitalized patients and out-patients treated in the first trimester of 1998. The strains were identified using the ATB computer system. Antibiotic sensitivity of the isolates was determined by disc-diffusion tests. ESBL production capacity of E. coli and K. pneumoniae strains was estimated by double-disc and ATB BLSA tests. Most of the analysed E. coli strains were found to exhibit significant sensitivity to compound penicillin preparations containing ß-lactarn inhibitor (Augmentin, Tazocin) and to the third generation cefalosporins, in contrast, K. pneumoniae strains much more frequently were resistant to the drugs. Among the obtained isolates, 3 (2,2%) E. coli strains and 21 (40,4%) K. pneumoniae strains produced ESBL but all the isolates proved sensitive to imipenem. In evaluation of ESBL production-detecting tests, the double-disc test was found to be more reliable than ATB BLSA test.

10

Wplyw warunkow badania na ocene wrazliwosci Staphylococcus cohnii na antybiotyki beta-laktamowe

88%

Nowak T. , Szewczyk E. M.

Medycyna Doświadczalna i Mikrobiologia

|

2006

|

tom 58

|

nr 2

95-102

PL

Przy oznaczaniu wrażliwości Staphylococcus cohnii na antybiotyki ß-lakta- mowe zastosowanie warunków hodowli stymulujących mechanizmy oporności sprzyja eliminacji błędnych wyników, niezgodnych z dobrze sprawdzającymi się wynikami oznaczania meticylinooporności w krążkowym teście z cefoksytyną (FOX30).

EN

The high occurence of coagulase-negative staphylococci among bacteria responsible for hospital infections is unquestioned. Studies on the poorly-known novobiocin-resistant, coagulase-negative Staphylococcus cohnii were undertaken. The possibilities of optimizing conditions for determination of susceptibility to ß-lactam antibiotics of this species were researched. In the case of S. cohnii the new cefoxitin test for detection of methicillin resistant strains, introduced by the National Reference Centre for Antibiotics in Poland was found as a good and of credible quality. It was also shown, that application in in vitro examination conditions stimulating the mechanisms of resistance to ß-lactam antibiotics, supplies credible results relating to their true susceptibility. The necessity of establishing individual conditions for susceptibility determination in different species of coagulase-negative staphylococci was suggested.

11

Znaczenie drobnoustrojow z rodzaju Mycoplasma w drogach moczowo-plciowych

75%

Root Kustritz M. V.

Weterynaria po Dyplomie

|

2010

|

tom 11

|

nr 1

64

12

Analiza profilu drobnoustrojow wyosobnionych od biorcow nerki z Kliniki Transplantologii i Chirurgii Ogolnej SPSK AM w Bydgoszczy w 2001 roku

75%

Michalska A. , Gospodarek E. , Wlodarczyk Z. , Gierlotka K.

Medycyna Doświadczalna i Mikrobiologia

|

2003

|

tom 55

|

nr 3

271-276

PL

Celem pracy była analiza profilu drobnoustrojów izolowanych od biorców nerki. Ogółem pobrano 140 próbek od 53 pacjentów i 40 próbek płynu konserwującego Euro-Colins. Obecność drobnoustrojów wykazano w 125 (69,4%) próbkach. Bakterie Gram-dodatnie stanowiły 58,4%, Gram-ujemne - 34,2%, a grzyby – 7,4%.

EN

One of the most important reasons of complications after organ transplantation may be the infections. The aim of the present work was to analyse of microorganisms isolated from patients, which were the recipients for kidney transplantation in 2001 year. The diagnostic material contained 140 samples from 53 patients, 40 (22,2%) samples from Euro-Collins fluid used for kidney storage before the transplantation and 3 end-pieces of catheter. The positive cultures were found in 125 (69,4%) samples. Gram-positive bacteria constituted 58,4%, Gram-negative bacteria - 34,2%, fungi - 7,4%. 140 strains of microorganisms were isolated from pharyngeal swabs and 55 strains of bacteria were isolated from palm swabs. Most of them were considered as a physiological flora. It was found 4-time significant bac- teriuria among positive cultures from urine samples. In the cultures of fluid used for kidney storage 12 (30,0%) positive samples were obtained, out of which 16 strains of microorganisms were isolated. Among the strains of Staphylococcus 35,3% were MR. Among 18 strains of Gram-negative rods one strain was multiresistant to antibiotics. None of analyzed strains was ESßL-producing. A high percentage of positive cultures from fluid used for kidney storage suggests the possibility of contamination of the organ with bacteria coming from kidney donor or during the storage, transport and actions connected with taking the organ to the transplantation.

13

Nabywanie lekoopornosci przez paleczki Salmonella w warunkach in vitro

75%

Skowron M. , Bos M. , Rzedzicki J.

Medycyna Weterynaryjna

|

2003

|

tom 59

|

nr 08

696-701

14

Wykrywanie i ocena wlasciwosci krajowych izolatow Arcanobacterium pyogenes wyosobnionych od swin

75%

Pejsak Z. , Markowska-Daniel I. , Samorek M. , Truszczynski M.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 07

781-784

EN

The aim of the study was the genetic identification and evaluation of properties of field isolates of A. pyogenes. Samples of internal organs as well as pathologically changed joints taken from dead pigs bred in Poland were used for testing. The isolation of bacteria was carried out according to standard microbiological methods. Properties of bacteria were evaluated based on their color, size and shape on the agar plates and in smears stained with the Gram method as well as on results of biochemical tests. Genetic identification of isolates based on results of the elaborated PCR test. Sensitivity to the most commonly used chemotherapeutics was evaluated by the disc diffusion test. Monocultures of transparent, small bacteria of pinhead size, surrounded by large hemolysis zone, Gram positive, fermented with acid production of glucose, fructose, galactose, lactose, cellobrose, trecholose, maltose, melarytose, mannose, glikogen, xylose and starch, of proteolytic activity, were evidenced. Tested A. pyogenes isolates were 100% sensitive to a wide spectrum of beta-lactames, with the exception of penicillin, for which 5% of the strains were resistant. One hundred percent of isolates were sensitive to oxytetracycline, doxycyclin, enrofloxacin, tylosin and florfenicol and 95% of them were sensitive to tetracyklines, cephalosporin and Linco-Spectin. A high percentage (90%) of sensitivity was registered for tiamulin, tulatromycyn, gentamicin and norfloxacin. The highest resistance was evidenced in the case of neomycin (47% resistant strains) and sulphonamides with trimetoprimem (45% of resistant strains). In PCR the presence of genetic material of A. pyogenes was detected in the case of 56 tested strains (98.2%).

15

Metoda krążkowo-dyfuzyjna w weterynaryjnej diagnostyce bakteriologicznej - praktyczne dane

75%

Borowska D. , Jablonski A. , Pejsak Z.

Życie Weterynaryjne

|

2014

|

tom 89

|

nr 02

16

Wrazliwosc klinicznych szczepow paleczek Gram-ujemnych na wybrane antybiotyki beta-laktamowe

75%

Rokosz A. , Sawicka-Grzelak A.

Medycyna Doświadczalna i Mikrobiologia

|

1997

|

tom 49

|

nr 3-4

191-198

EN

The aim of the study was to determine the activity of four beta-lactam antibiotics against nosocomial strains of gram-negative bacilli. Two antibiotics combined with beta-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in the following wards: surgery and intensive care unit of State Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 E and ID 32 GN strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB G- and ATB PSE strips. The susceptibility of the strains to imipenem, meropenem, timentin and tazocin was tested by disc-diffusion method. 157 strains of gram-negative bacilli were cultured. 100 strains were isolated from patients hospitalized in surgical ward and 57 strains from patients hospitalized in ICU. Nonfermenting rods dominated among isolated strains -91. The results obtained indicate that multiresistant gram-negative rods causing serious therapeutic problems are often isolated from clinical specimens. The contribution of nonfermenting rods, especially Pseudomonas spp. and Atinetobacter spp. to the etiology of infections in hospitalized patients has increased. Infections caused by these strains are difficult to cure. Tazocin and carbapenems (imipenem and meropenem) are highly active in vitro against the examined strains of gram-negative bacilli.

17

Wrazliwosc drobnoustrojow wyizolowanych z mleka cwiartkowego krow na antybiotyki

75%

Borkowska D. , Bak M.

Przegląd Hodowlany

|

2006

|

tom 74

|

nr 10

8-10

18

Oddzialywanie fungicydow i antybiotykow na przezywalnosc Rhizobium lupini i brodawkowanie Lupinus albus L.

75%

Piotrowski W. , Stefaniak O. , Slizak W.

Materiały ... Sesji Naukowej Instytutu Ochrony Roślin

|

1992

|

tom 32

|

nr 1

151-159

19

Sanfetrinem, przedstawiciel trinemow - aktywnosc in vitro wobec szczepow Klebsiella pneumoniae wytwarzajacych ESBL

75%

Wieczorek P. , Sacha P. T. , Leszczynska K. , Jakoniuk P. , Zalewska M.

Medycyna Doświadczalna i Mikrobiologia

|

2006

|

tom 58

|

nr 1

27-32

PL

Zbadano wrażliwość 43 szczepów Klebsiella pneumoniae wytwarzających ESBL wobec sanfetrinemu. Uzyskane wyniki porównano z wynikami dla imi- penemu (MIC50, MIC90). Wszystkie izolaty były wrażliwe na imipenem i cefe- pim, a oporne na gentamycynę, amikacynę i trimetoprim/sulfametoksazol.

EN

Sanfetrinem is the first member of tricyclic ß-!actams (trinems) which can be administered orally as a hexatil ester. His chemical structure is related to carbapenems. High stability to many ß-lactamases and human renal dehydropeptydase were described. The investigation was performed on 43 strains of Klebsiella pneumoniae producing ESBL isolated from hospitalized patients. The MICs of sanfetrinem and imipenem were determined by E-test. Additio- naly, susceptibility to antibiotics was tested by disc diffusion method. Klebsiella pneumoniae ATCC 700603, Escherichia coli ATCC 25922 and Escherichia coli ATCC 35218 were used as a control strains. MIC50 and MIC90 of sanfetrinem and imipenem amounted respectively 0,38/3 mg/l and 0,19/0,25 mg/l. Range of MIC value was from 0,064 mg/l to 4 mg/l for sanfetrinem, and from 0,094 mg/l to 0,38 mg/l for imipenem. Additionaly, geometric and aritmetic means were calculated to both antibiotics. All results of study were compared using correlation factor and „Student" t-test. None of these 43 Klebsiella pneumoniae strains was resistant to imipenem and cefepime. Majority of isolates demonstrated susceptibility to ciprofloxacin and cefoxitin - 90,7% and 74,4% respectively. All strains were resistant to gentamicin, amikacin and trimethoprim/sulfamethoxazole.

20

Wrazliwosc na antybiotyki bakterii Streptococcus sp. izolowanych z mleka przezuwaczy

75%

Vasil M. , Zapletal P.

Prace i Materiały Zootechniczne

|

2008

|

tom 65

115-119