Wyniki wyszukiwania - Biblioteka Nauki

1

Zmiennosc i ewolucja wirusa pryszczycy

100%

Siwicki A. K. , Szweda W. , Platt-Samoraj A. , Malaczewska J.

Medycyna Weterynaryjna

|

2002

|

tom 58

|

nr 12

938-941

2

Badania molekularne serotypow wirusa pryszczycy

100%

Paprocka G.

Medycyna Weterynaryjna

|

2007

|

tom 63

|

nr 07

778-782

EN

The foot-and-mouth disease virus (FMDV) belongs to the family of Picornaviridae, genus Aphthovirus. The virus exists in the form of seven different serotypes: O, A, C, Asia 1, SAT 1, SAT 2, SAT 3 and multiple subtypes reflecting significant genetic variability. The FMDV genome organization is similar to that of other picornaviruses. The determination of FMDV nucleotide sequences and phylogenetic analysis is the definitive technique for characterizing individual isolates of the virus. The paper presents information regarding genetic studies of FMDVs originating from different parts of the world.

3

Wirus pryszczycy i jego budowa molekularna

88%

Paprocka G.

Medycyna Weterynaryjna

|

2006

|

tom 62

|

nr 07

753-756

EN

Foot-and-mouth disease virus (FMDV) is an important animal pathogen that belongs to the Aphthovirus genus of the Picornaviridae family and infects cattle and other cloven-hoofed animals. Seven serotypes (A, O, C, Asia1, SAT1, SAT2 and SAT3) have been identified serologically, and multiple subtypes occur within each serotype. FMDV enters cells by receptor-mediated endocytosis. By electron microscopy the FMD virion appears to be a round particle with a smooth surface and a diameter of about 25 nm. The FMD viral particle contains a positive-strand RNA genome of about 8500 nucleotides, enclosed within a protein capsid. The virus capsid is made up from 60 copies each of four virus-encoded proteins VP1 to VP4. The FMDV genome is composed of the 5’ non-translated region (5’NTR), the coding region, and the 3’ non-translated region (3’NTR). The genome encodes a single polyprotein, from which the different viral polypeptides are derived by viral proteases. FMDV populations are genetically and anti-genetically heterogeneous. FMDV have very high mutation rates.

4

Zastosowanie wirusów jako broni biologicznej przeciwko zwierzętom gospodarskim

75%

Kielan A. , Niemialtowski M.

Medycyna Weterynaryjna

|

2014

|

tom 70

|

nr 04

EN

Bioterrorist attacks could be catastrophic not only for the people (bioterrorism), but also for animals and plants (agroterrorism) and for economy of each country (national security). In this paper we have focused on two viruses extremely important from the veterinary point of view: (i) foot-and-mouth disease virus (FMDV; virus RNA) and (ii) African swine fever virus (ASFV; virus DNA), that are excellent examples of very dangerous infectious agents that can be used as a biological weapon for agroterrorist attacks.

5

Wykrywanie wirusa pryszczcycy w materiale biologicznym

75%

Paprocka G. , Kesy A.

Medycyna Weterynaryjna

|

2001

|

tom 57

|

nr 02

114-117

6

Zastosowanie sondy molekularnej do wykrywania wirusa pryszczycy

75%

Paprocka G. , Kesy A.

Medycyna Weterynaryjna

|

2003

|

tom 59

|

nr 03

227-229

7

Pryszczyca - nosicielstwo wirusa u przezuwaczy

75%

Niedbalski W. , Kesy A.

Medycyna Weterynaryjna

|

2004

|

tom 60

|

nr 02

115-118

8

Wykrywanie i serotypowanie izolatów wirusa pryszczycy

75%

Paprocka G. , Kesy A. , Niedbalski W. , Fitzner A.

Medycyna Weterynaryjna

|

2002

|

tom 58

|

nr 09

9

Przydatnosc jednowarstwowych hodowli komorek do wykrywania wirusa pryszczycy

75%

Paprocka G. , Kesy A. , Niedbalski W. , Fitzner A.

Medycyna Weterynaryjna

|

1994

|

tom 50

|

nr 06

271-274

EN

The growth power of different cell lines: calf kidney, heart fibroblasts, thymus and foetal testicles, as well as porcine kidney in 17 types of media prepared on the basis of Hanks, Eagle’s and Parker’s media were studied. The conditions for the preparation of cell suspensions for deep freeze storage and the optimal conditions for cell growth after thawing were determined. The titers and intensity of CPE od FMD virus, type A, О and C, propagated in cell cultures obtained directly after trypsinization and after 6 months of storing the cells in liquid nitrogen (-196°C), were similar in calf kidney cells, pig kidney cells and calf thyroid cells. No cyto-pathic effects were observed in cultures of calf heart and foetal calf testicles infected with FMDV type A, О and C.

10

Występowanie pryszczycy na świecie w latach 2010-2011 i na początku 2012 roku

75%

Paprocka G. , Fitzner A.

Medycyna Weterynaryjna

|

2013

|

tom 69

|

nr 01

EN

Foot and mouth disease (FMD) is the most contagious disease of domesticated and wild cloven-hoofed animals, caused by a virus of the Aphthovirus genus in the Picornaviridae family. Foot and mouth disease virus (FMDV) exists as seven different serotypes (O, A, C, Asia 1, SAT 1, SAT 2, SAT 3), which are not uniformly distributed across the globe. Six of the seven serotypes of FMD (O, A, C, SAT 1, SAT 2, SAT 3) occur in Africa, four (O, A, C, Asia 1) in Asia, and only three (O, A, C) in South America. FMD-endemic areas of the world are high-risk zones for introducing FMD to countries free of the disease. In 2010-2011 and at the beginning of 2012, FMD caused by different serotypes occurred in Asia, Africa and South America. It also occurs in Europe, in Bulgaria. Asia is still the main source of outbreaks for the Middle East and Europe. In the period considered, the dominant serotype was O, and no serotype C was observed. It is noteworthy that serotype C has not been recorded since 2004. Its disappearance has not yet been explained. This article presents key information on the outbreaks of FMD around the world based on the data from Office International des Epizooties (OIE) and the World Reference Laboratory for Foot-and-Mouth Disease (WRL FMD).

11

Zastosowanie hodowli pierwotnej komorek nerki jagniecia w diagnostyce pryszczycy

63%

Paprocka G.

Medycyna Weterynaryjna

|

2008

|

tom 64

|

nr 03

332-334

EN

Sensitive cell cultures, such as primary bovine thyroid cells and primary pig, calf or lamb kidney cells can be used for isolating foot-and-mouth disease virus (FMDV). Established cell lines IB-RS-2 and BHK-21 may also be applied for this purpose. The aim of this study was to assess the efficacy of primary lamb kidney cell culture for detecting FMDV in biological materials. The results of the study demonstrate that this cell culture may be a useful tool in diagnostic studies of FMD.

12

Izolacja i identyfikacja wirusa pryszczycy

63%

Paprocka G.

Medycyna Weterynaryjna

|

2008

|

tom 64

|

nr 12

1404-1406

EN

Foot-and-mouth disease (FMD) is the most contagious disease of mammals and has great potential for causing severe economic losses in susceptible cloven-hoofed animals. FMD is caused by a virus of the genus Aphthovirus, family Picornaviridae. Serological tests in laboratories have identified seven different serotypes as O, A, C, SAT 1, SAT 2, SAT 3 and Asia 1. FMD is diagnosed by the virus isolation or demonstration of FMD viral antigen or nucleic acid in samples of biological specimens. The purpose of the study was to apply the isolation test in cell culture and a RT-PCR assay for the detection of foot-and-mouth disease virus in biological materials. Out of the total of 14 examined samples, 6 (42.8%) were found positive using these methods. The antigen ELISA was used for the confirmation of specificity of the isolation assay. Primary bovine thyroid cells were found the most sensitive cell culture system for the detection of foot-and-mouth disease virus, followed by secondary lamb kidney and certain IB-RS-2 cells.

13

Przydatnosc RT-PCR w diagnostyce pryszczycy

63%

Paprocka G.

Medycyna Weterynaryjna

|

2009

|

tom 65

|

nr 09

621-623

EN

Foot-and-mouth disease virus (FMDV) from the family Picornaviridae, genus Aphthovirus, exists in the form of seven different serotypes: O, A, C, Asia 1 and SAT 1-3. Infection with one serotype does not confer immunity against another. Foot-and-mouth disease is one of the greatest threats to animal health in European countries. The rapid and accurate detection of FMDV is of the utmost importance. The RT-PCR assay was used to detect the presence of FMDV in samples. Positive results of the RT-PCR assay were found in all samples and in the positive control, the negative control reacted negatively. No cytopathic effects in primary bovine thyroid cells were observed in 2 samples that had been thawed several times. The reference strains of FMDV was used to determine the sensitivity of the test. The sensitivity of RT-PCR for detection of FMDV (serotype O, A) was 1 TCID₅₀ and 10 TCID₅₀ (serotype C, Asia 1) by gel electrophoresis.

14

Cykl infekcyjny wirusa pryszczycy

63%

Paprocka G.

Medycyna Weterynaryjna

|

2009

|

tom 65

|

nr 04

232-236

EN

Foot-and-mouth disease virus (FMDV) is a single-stranded, positive-sense RNA virus belonging to the genus Aphthovirus in the family Picornaviridae. FMDV enters cells via the mechanism of receptor-mediated endocytosis in which the low pH of the endosomal compartment triggers uncoating of the viral genome. FMDV enters cells by attaching itself to cellular receptor molecules of the integrin family. For FMDV the receptor has been identified as the Arg-Gly-Asp (RGD) binding integrin. The integrin-binding RGD is located in the G-H loop of VP1 and it is highly conserved among all seven serotypes. The FMDV genome organization is similar to that of other picornaviruses. The genome is composed of three parts, the 5’ non-translated region (5’NTR), the coding region and the 3’ non-translated region (3’NTR) containing a heteropolymeric segment and poly(A) tail, which is required for viral replication. The 5’NTR plays important roles in cap-independent translation initiation of the viral polyprotein and in viral genome replication. After translation, the polyprotein is cleaved into four primary cleavage products: the amino terminal L protease; P1-2A, the precursor of the capsid proteins; 2BC and P3 which are cleaved into nonstructural proteins.

15

Patogeneza pryszczycy

63%

Paprocka G.

Medycyna Weterynaryjna

|

2008

|

tom 64

|

nr 10

1180-1183

EN

Foot-and-mouth disease (FMD) is a highly contagious viral vesicular disease of cloven-hoofed animals of the Artiodactyla order. The disease is characterized by fever, lameness and vesicular lesions on the tongue, feet, snout and teats. It is generally accepted that primary infection of ruminants usually occurs by the respiratory route, whereas pigs are usually infected by the oral route. Pigs are much less susceptible to aerosol infection than cattle, yet they excrete far more aerosolized virus than cattle or sheep. In addition, cattle, sheep, and goats can become carriers. The virus elicits a rapid humoral response in either infected or vaccinated animals Virus-specific antibodies protect animals in a serotype-specific manner against reinfection, or against infection in the case of vaccination. Protection is correlated with a high levels of neutralizing antibodies. The role of cellular immunity in the protection of animals from FMD is still a matter of some controversy.

16

Pryszczyca i choroba pecherzykowa swin - roznicowanie metoda PCR

63%

Paprocka G. , Kesy A. , Niedbalski W. , Fitzner A.

Medycyna Weterynaryjna

|

1999

|

tom 55

|

nr 08

539-541

17

Pryszczyca - aktualne metody diagnostyczne

63%

Paprocka G. , Kesy A. , Niedbalski W. , Fitzner A.

Biotechnologia

|

1997

|

nr 1

131-137

18

Postęp w dziedzinie badań nad pryszczycą

63%

Paprocka G. , Kesy A.

Postępy Mikrobiologii

|

1986

|

tom 25

|

nr 1-2

19

Sytuacja epidemiologiczna pryszczycy w latach 2014-2015 i na początku 2016 r.

63%

Paprocka G. , Kesy A.

Medycyna Weterynaryjna

|

2017

|

tom 73

|

nr 03

EN

This article presents key information about foot-and-mouth disease (FMD) outbreaks around the world, based on data from Office International des Epizooties (OIE), World Reference Laboratory for Foot-and-Mouth Disease (WRL FMD) and the European Laboratory for Foot and Mouth Disease (EURL) at the Pirbright Institute. In the years 2014-2015 and early 2016, FMD caused by immunologically diverse serotypes O, A, Asia 1, SAT 1, SAT 2, SAT 3 occurred in areas of Asia and Africa, but there were no new outbreaks of the disease in South America. Within this period of time the dominating serotype was serotype O. For many years there were no reports about outbreaks caused by serotype C, the last of them occurring in 2004 (Brazil, Kenya). Significant epidemiological events were related to spreading of the virus serotype O (ME-SA/Ind-2001) and A (ASIA/G-VII(G-18)) from the Indian subcontinent to new regions. Serotype O (ME-SA/Ind-2001) spread in the years 2013-2015 in the Middle East (United Arab Emirates, Saudi Arabia, Bahrain) and North Africa (Libya, Tunisia, Algeria, Morocco); it was also detected in 2015 in Southeast Asia (Laos). In turn, the serotype A (ASIA/G-VII(G-18)) was recorded in 2010 in Myanmar, and in 2015 appeared in the Middle East (Saudi Arabia, Iran, Armenia, Turkey). Those events constituted a threat to neighbouring countries and increased the risk of intrusion FMD to Europe. A reason for concern was also given by the numerous outbreaks of foot-and-mouth disease in South Korea, caused by serotype O (SEA/Mya-98). In European countries there have been no outbreaks since 2011 (Bulgaria). For the record, the last outbreak in Poland was identified 45 years ago, in 1971.

20

Pryszczyca - stale zagrozenie epidemiologiczne

63%

Kesy A. , Paprocka G. , Niedbalski W. , Fitzner A.

Medycyna Weterynaryjna

|

2001

|

tom 57

|

nr 06

379-382