2-/4-/2-(4-chlorbenzamido) ethyl/phenoxy/-2-methyl-propionic acid (CBMP) reduced the intensity of cytopathic effect, delayed the time of its appearance for 24—48 hours and finally caused a significant lowering of ТСID₅₀ titer of herpes simplex in monkey kidney cell cultures and of pseudorabies virus in chick embryo cell cultures. On the other hand CBMP did not exert any effect on the multiplication of Roakin strain of NDV in chick embryo cell cultures. The preparation did not inactivate directly the viruses being examined and did not influence their adsorption. It is suggested that antiviral effect of CBMP is due to disordering the lipid metabolism in cells what causes the disturbances of virus envelope synthesis.
Analizowano humoralną odpowiedź immunologiczną indukowaną przez mutant temperaturowrażliwy wirusa herpes simplex typu - 1 (mutant ts HSV-1). Określono poziom przeciwciał w klasie IgG oraz przeciwciał wiążących dopełniacz dla HSV-1 w surowicach świnek morskich szczepionych mutantem ts HSV-1 na różnych poziomach pasaży, co pozwoliło na ocenę stabilności aktywności immunogennej mutanta. Porównano również przyrost poziomu przeciwciał dla mutanta ts HSV-1 i szczepu natywnego.
EN
The aim of the present study was the analysis of the immunogenic activity of temperature sensitive mutant of HSV-1 with significantly decreased pathogenicity for mice. The stability of immunogenic potency was also tested. The level of antibody - mediated response in guinea pigs after two doses of mutant ts HSV-1 or native strain was compared. Antibodies to HSV were measured using ELISA and CFM. Seroconversion rates in tested groups were high, from 80% to 100%. The level of antibody response at 4 week after inoculation measured by ELISA and CFM showed no significant differences beetwen 4 passages of mutant ts HSV-1. Significant higher increase of antibodies level after two doses of viruses was observed for mutant to HSV-1 as compared to native strain, both in ELISA and CFM.
Analizowano humoralną odpowiedź immunologiczną, indukowaną przez mutant temperaturowrażliwy wirusa herpes simplex typu - 1 (mutant ts HSV-1) w obecności innych atenuowanych szczepów wirusowych. Zbadano również wpływ mutanta ts HSV-1 na odpowiedź immunologiczną, indukowaną przez inne szczepy wirusowe, wchodzące w skład poliwalentnych układów szczepienia (atenuowane szczepy wirusa odry i wirusa nagminnego zapalenia przyusznic).
EN
The ability of mutant is HSV-1 on 45 passage to induction of IgG antibody response in the presence of other attenuated viral strain, like measles, mumps, rubella was evaluated, and the effect of mutant to HSV-1 on humoral immunity induced by attenuated measles and mumps strains was also tested. The obtained results clearly indicated that even immunization with other RNA virus strains had no effect on IgG antibody response ta mutant ts HSV-J. Significant differences were observed in IgG antibody responses to mumps and measles between groups of guinea pigs immunized with these viruses alone or together with other viruses. This was especially observed in the case of mumps virus. It clearly shows that change of virus concentration rates in polyvalent schema of immunization is needed.
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