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Assessment of DNA damage using chromosomal aberrations assay in lymphocytes of waterpipe smokers

100%

Alsatari E. S. , Azab M. , Khabour O. F. , Alzoubi K. H. , Sadiq M. F.

nr 3

218-224

Objectives: The aim of this study was to investigate the genotoxicity of waterpipe smoking in the lymphocytes of waterpipe smokers using chromosomal aberrations (CAs) assay. Materials and Methods: Fifty waterpipe smokers and 18 healthy nonsmokers volunteered to participate in the study. Additionally, 18 heavy cigarette smokers were recruited for comparison. Chromosomal aberrations (CAs) assay was used to evaluate DNA damage in the lymphocytes. Results: The results showed that similarly to cigarette smoking, waterpipe smoking significantly increased the frequencies of CAs (p < 0.01). In addition, the frequencies of CAs increased with more waterpipe use. Conclusions: Waterpipe smoking causes DNA damage to lymphocytes and the damage increases with more waterpipe use.

Waterpipe. The rethinking of an ethnical practice’s meaning in city

88%

Nagy V.

tom 41

nr 3

211–221

The paper attempts to examine the multicultural society of Budapest in the context of the Oriental tradition brought by the Arab, Turkish and Persian immigrants. The author presents the smoking of waterpipe as an object of a transfer from immigrants custom into general fashion. According to the author, by using sheesha the transmitters make effort to create familiarity and feeling like at home. Nevertheless, this practice dislodged from the original context (or users) and became a fashionable mean to create or to support social circles. This is a good example of general question of a process by which ethnic traditions and customs become parts of life in multicultural environment of big cities nowadays.

Simple HPLC method for simultaneous quantification of nicotine and cotinine levels in rat plasma after exposure to two different tobacco products

75%

Alhusban A. A. , Hammad A. M. , Alzaghari L. F.

tom Vol. 35, no. 1

106--114

Purpose: Development and validation of a selective analytical method to accurately and precisely quantify nicotine and cotinine levels in rat's plasma after exposure to tobacco cigarettes and tobacco water-pipe. Methods: An easy HPLC-Photodiode-Array Detection (PDA) method was developed and validated for simultaneous determination of nicotine and cotinine levels in plasma of 15 rats (10 rats after tobacco products exposure and 5 control rats). Nicotine and cotinine were extracted in one step from plasma using acetonitrile and concentrated to lowest volume using nitrogen stream. Results: The developed method offered a rapid analysis time of 14 min with single step of analytes extraction from rat's plasma with recovery percentage range between 93 and 95% and excellent linearity with correlation factor more than 0.994 with analytical range between 50 and 1000 ng mL⁻¹ and LOD of 25 ng mL⁻¹ and 23 ng mL⁻¹ for nicotine and cotinine, respectively. The analysis of rat's plasma after 28 days of exposure to tobacco cigarettes and tobacco water-pipe revealed that the average concentrations of 376 ng mL⁻¹ for cotinine and 223 ng mL⁻¹ for nicotine were obtained after tobacco cigarettes exposure, and 220 ng mL⁻¹ for cotinine and 192 ng mL⁻¹ for nicotine after tobacco water-pipe exposure. Conclusion: Higher nicotine and cotinine levels were found in plasma after tobacco cigarettes exposure than water-pipe exposure which may have potential undesirable effects on passive smokers in both cases.