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Content available remote The post-haemorrhagic vasopressin release into the blood
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EN
The aim of the present study was to compare the influence of the renin-angiotensin and sympathetic system in the process of post-haemorrhagic vasopressin release. A dialysis of the venous blood from the sella turcica region was performed in male rats under anaesthesia. The animals were divided into eight experimental groups: 1) control; 2) bleeding; 3) 20 days after superior cervical ganglionectomy; 4) 20 days after superior cervical ganglionectomy and bleeding; 5) injection of captopril; 6) injection of captopril and bleeding; 7) 20 days after superior cervical ganglionectomy and injection of captopril; 8) 20 days after superior cervical ganglionectomy, injection of captopril and bleeding. The content of vasopressin in dialysates was determined by radioimmunoassay. In control rats the release of vasopressin into dialysates was constant during 180 min of the experiment. Bleeding, as well as, superior cervical ganglionectomy caused an increase in vasopressin release. Captopril did not change vasopressin release in comparison to control group. Furthermore, vasopressin release after both, bleeding and sympathetic denervation performed simultaneously was significantly abolished. We conclude that renin-angiotensin, as well as, sympathetic nervous system are involved in the increased post-haemorrhagic vasopressin release.
EN
The acid-base equilibrium is closely linked to gas exchange in the lungs, and respiratory exchange ratios are used to evaluate respiratory effectiveness and tissue oxygen levels. Acid-base indicators are determined in both arterial and venous blood samples. This study compares the usefulness of acid-base indicators of venous and arterial blood in monitoring the condition of horses with recurrent airway obstruction. Prior to treatment involving bronchodilating glucocorticoids, expectorant and mucolytic drugs, more pronounced changes were observed in venous blood (pH 7.283, pCO2 61.92 mmHg, pO2 35.541 mmHg, HCO3 - 31.933 mmHg, BE 2.933 mmol/l, O2SAT 58.366%, ctCO2 38.333 mmol/l) than in arterial blood (pH 7.309, pCO2 53.478 mmHg, pO2 90.856 mmHg, HCO3 - 28.50 mmHg, BE 3.133 mmol/l, O2SAT 93.375%, ctCO2 31.652 mmol/l), indicating compensated respiratory acidosis. The improvement of respiratory efficiency minimized acidosis symptoms in both venous blood (pH 7.365, pCO2 43.55 mmHg, pO2 47.80 mmHg, HCO3 - 30.325 mmHg, BE 3.050 mmol/l, O2SAT 80.10%, ctCO2 29.80 mmol/l) and arterial blood (pH 7.375, pCO2 39.268 mmHg, pO2 98.476 mmHg, HCO3 - 26.651 mmHg, BE 4.956 mmol/l, O2SAT 98.475%, ctCO2 28.131 mmol/l). Venous blood parameters were marked by greater deviations from mean values, both before and after treatment. Acid-base indicators determined in venous blood are indicative of respiratory disturbances, but they do not support a comprehensive evaluation of gas exchange in the lungs.
EN
The aim of the present study was to estimate the absorption of 125I-labeled proinflammatory cytokines - interleukin-lß (IL-lß), interleukin-6 (IL-6) and tumor necrosis factor-a (TNF-a) from inflamed porcine uterus into the uterine venous blood. Moreover, in order to test the hypothesis that the above cytokines penetrate directly into ovaries and oviduct via local destination transfer in the area of the ovarian vascular pedicle and bypassing the systemic circulation, the concentration of IL-lß, IL-6 and TNF-a in ovarian and oviductal tissues was also studied. These cytokine concentrations were also estimated in the ovarian venous blood. IL-lß, IL-6 and TNF-a from both control and inflamed uteri were absorbed into the uterine venous blood, but it was higher (P < 0.05-0.001) from the pathologically changed uteri. The uterine tissues, particularly the endometrium, of both control and inflamed uteri retained all studied cytokines, but to a higher degree (P < 0.001) in the inflamed uteri. Injections of IL-lß, IL-6 and TNF-a into the control and inflammatory changed uteri produced the presence of these proteins in the ovary and oviduct. However, the concentrations of IL-lß and IL-6 in the ovarian and oviductal tissues was low after injections of control and inflamed uteri with these cytokines. In turn, administration of TNF-a into the inflammatory changed uteri lead to an enhancement in the concentration of this cytokine in the ovarian parenchyma (P < 0.05) and oviduct (P < 0.001). All studied cytokines were found in the ovarian venous blood after their injection into both control and inflamed uteri, which indicated its local destination transfer to the ovary. However, the concentration of cytokines increased (P < 0.05-0.001) in the gilts with pathologically changed uteri as compared to controls. The study showed that both control and inflamed porcine uteri absorbed IL-lß, IL-6 and TNF-a into the uterine venous blood, but the values of absorbed cytokines from inflamed uteri were higher. Moreover, the quantity and the manner of the studied cytokines absorption into the uterine venous blood differed.
PL
W pracy przedstawiono wyniki badań własnych dotyczących przeciwprądowego przenikania z krwi żylnej i limfy do krwi tętniczej progesteronu, oksytocyny i prostaglandyny F2α w obszarze więzadła szerokiego macicy u krów będących w fazie lutealnej cyklu estralnego i na tym tle rozpatrzono znaczenie tego procesu w regulacji rozrodu u krowy. Progesteron wytwarzany przez ciałko żółte w czasie fazy lutealnej opuszcza jajnik z krwią żyły jajnikowej i limfą. W obszarze naczyń krezki jajnika (mesovarium) ulega przeciwprądowemu przenikaniu do krwi tętnicy jajnikowej. Powstaje zwiększona lokalna koncentracja progesteronu w tym obszarze, a przez gałąź maciczną tętnicy jajnikowej rów­nież w obszarze macicy i jajowodu. Sugeruje się, że tą drogą zwiększona lokalnie koncen­tracja progesteronu bierze udział w przygotowaniu macicy do spełnienia funkcji wydzielniczej, a szczególnie ma to znaczenie w sekrecji PGF2α. Wykazano również przeciwprądowe przenikanie i transport jajnikowej oksytocyny z krwi żylnej odpływającej z jajnika do krwi tętniczej zaopatrującej zarówno jajnik, jak i przez odgałęzienie tętnicy jajnikowej - macicę i jajowód. Sugeruje się, że lokalna koncentracja oksytocyny w obszarze macicy i jajowodu ma na celu zapoczątkowanie zmian, zarówno naczyniowych, jak i wydzielniczych, związanych ze zbliżającą się luteolizą. Wykazano przeciwprądowe, zwrotne przenikanie PGF2α z krwi żylnej do krwi tętniczej w obszarze krezki macicy (mesometrium), przy czym proces ten z większą intensywnością przebiegał po stronie ciałka żółtego. Proces ten ma za zadanie ochronę aktywnego ciałka żółtego przed luteolizą.
EN
The paper presents the results of own investigations concerning counter-current transfer of progesterone, oxytocin and prostaglandin F2α from venous blood and lymph to arterial blood in the area of broad ligament of uterus in the case of cows going through the luteal phase of estral cycle. The significance of the above-mentioned process in regulation of cow reproduction was examined. Progesterone produced by corpus luteum during the luteal phase leaves the ovary with ovarian vein blood and lymph. It is then subjected, in the area of mesovarium, to counter-current transfer to the blood of ovarian artery. It causes an increase in local concentration of progesterone both in this area and, through the uterine branch of ovarian artery, in those of uterus and oviduct. It may suggest that in this way the locally increased concen­tration of progesterone participates in the preparations of uterus to performing the func­tion of secretion, it is especially important in the case of PGF2α secretion. It was also shown that counter-current transfer and transport of ovarian oxytocin from venous blood, flowing from the ovary, to arterial blood supplying both the ovary and, through the branch of ovarian artery, the uterus and oviduct, takes place as well. It seems that the local concentration of oxytocin in the area of uterus and oviduct is aimed at starting some changes in vasculature and secretion, connected with approaching luteolysis. Counter-current back transfer of PGF2α from venous to arterial blood in the area of mesometrium was also observed. However, this process was more intensive on the side ipsilateral to corpus luteum. It is aimed at protection of active corpus luteum against luteolysis.
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