A three-step procedure was adopted for the induction of gynogenesis in two cultivars of mulberry (Morus alba L.). This includes in vitro flowering, inflorescence segment culture and isolated ovary culture. In the third step, that is, isolated ovary culture, the cultured ovaries burst and an embryo emerged from the ovary. The present paper investigates the ontogeny of the developing gynogenic embryo. The study confirmed that the gynogenic embryo emerged from the egg cell. Before the onset of division of the egg, all other cells of the embryo sac degenerated in the majority of ovules, but in exceptional cases the polar nuclei will be retained along with the dividing egg cell. The presence of the gynogenic embryo along with free-nuclear autonomous endosperm is the most important feature of the present investigation. Autonomous endosperm is formed from either the polar nuclei or secondary nucleus. In both cultivars used for the experiment, the percentage of ovaries showing proembryo induction during inflorescence segment culture is much higher than that of ovaries producing gynogenic plants during isolated ovary culture. This suggests the degeneration of some gynogenic embryos during the initial stages of induction.
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