Wyniki wyszukiwania - Biblioteka Nauki

1

Transcellular electrical profiles of free cells of Malus domestica

100%

Karcz W.

Acta Biologica Silesiana

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1990

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tom 14

2

Winter hardiness performance of micropropagated sour cherry cultivars

88%

Borkowska B. , Zraly B.

Fruit Science Reports

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1987

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tom 14

|

nr 4

3

GC-MS Analysis of the Essential Oil from Flowers of Chrysanthemum coronarium L. Propagated Conventionally and Derived from In Vitro Cultures

88%

Wesołowska A. , Grzeszczuk M. , Kulpa D.

Acta Chromatographica

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2015

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tom Vol. 27, no. 3

525--539

EN

The composition of the essential oils obtained by hydrodistillation from flowers of Chrysanthemum coronarium L. has been investigated by gas chromatography-mass spectrometry (GC-MS). A total of 105 different constituents were identified, representing an average of 97.71–99.95% of the total oil composition. The oil obtained from flowers propagated conventionally contained the higher amounts of cis-chrysanthemol, cischrysanthenyl isovalerate, camphor, and virdiflorol, while the higher concentration of bornyl acetate, cis-chrysanthenyl acetate, and 2-(2,4-hexadiynylidene)-1,6-dioxaspiro[4.4] non-3-ene was noted in the oil obtained from flowers propagated in vitro. Significantly higher amounts of essential oil were obtained from flowers of plants derived for in vitro propagation (0.58%) in comparison with conventional cultivation (0.50%).

4

A mathematical model of HIV-1 infection including the saturation effect of healthy cell proliferation

88%

Kouche M. , Ainseba B. e.

International Journal of Applied Mathematics and Computer Science

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2010

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tom 20

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nr 3

601-612

EN

In this paper we derive a model describing the dynamics of HIV-1 infection in tissue culture where the infection spreads directly from infected cells to healthy cells trough cell-to-cell contact. We assume that the infection rate between healthy and infected cells is a saturating function of cell concentration. Our analysis shows that if the basic reproduction number does not exceed unity then infected cells are cleared and the disease dies out. Otherwise, the infection is persistent with the existence of an infected equilibrium. Numerical simulations indicate that, depending on the fraction of cells surviving the incubation period, the solutions approach either an infected steady state or a periodic orbit.

5

Secondary metabolites from plant cell and tissue cultures

88%

Hoopen H. J. G. , Verpoorte R.

Biotechnologia

|

1997

|

nr 2

6

Effect of culture medium and illumination on the acid invertase activity in callus of Medicago strasseri

88%

Medina M. , Villalobos N. , De La Cruz P. J. , Dorado A. , Guerra H.

Acta Physiologiae Plantarum

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1999

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tom 21

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nr 2

EN

The different acid invertase activity (total, soluble, wall-bound and extracellular) in calli induced on explants (cotyledon, petiole, hypocotyl and leaf) originated from Medicago strasseri seedlings were evaluated. In cultures subjected to 16 h photoperiod, the highest total, soluble and extracellular activities were found in calli from leaves cultured in medium 12 (MS with 0.01 mg·dm⁻³ (0.045 µM) of TDZ), elevated amounts of total and wall-bound invertase being found in calli induced on petioles in 12G medium (MS with 0.01 mg·dm⁻³ (0.045 µM) TDZ and 3.104 mg·dm⁻³ glycerol). In cultures maintained in darkness, the activity detected was lower than that observed in cultures under light conditions. The highest amounts of enzyme was bound in calli cultured on medium 12 (total and extracellular invertase) -leaves- and medium 12D (MS with 0.001 mg·dm⁻³ (0.0045 µM) TDZ) (soluble invertase) -using hypocotyls. In general, the different forms of invertase activity studied seem to appear in greatest amounts in calli induced under light conditions using leaves as explant and TDZ as growth regulator.

7

Apomictic phenomena in plant tissue culture

88%

Czapik R. , Koscinska-Pajak M.

Acta Biologica Cracoviensia. Series Botanica. Supplement

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2000

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tom 42

|

nr 1

8

Comparison of growth of rhododendrons propagated by grafting, tissue culture and from cuttings

88%

Muras P.

Folia Horticulturae

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1992

|

tom 04

|

nr 1

9

Escape from contact inhibition as a result of signal transduction disorder

88%

Janik P. , Miloszewska J. , Przybyszewska M.

Cellular and Molecular Biology Letters

|

2002

|

tom 07

|

nr Suppl.

10

Changes in the uptake of selected metabolites in relation to differentiation in Brassica napus callus cultures

88%

Zur I. , Skoczowski A. , Pienkowski S.

Acta Physiologiae Plantarum

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1997

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tom 19

|

nr 2

11

Regulation of the differentiated phenotype in vitro

88%

Freshney R. J.

Folia Histochemica et Cytobiologica. Supplement

|

1997

|

tom 35

|

nr 2

12

Methods of eliminating contaminations in tissue cultures on the example of selected ornamental plant species

75%

Parzymies M. , Dabski M.

BioTechnologia. Journal of Biotechnology Computational Biology and Bionanotechnology

|

2015

|

tom 96

|

nr 1

13

Effect of nanosilver on potato plant growth and protoplast viability

75%

Ehsanpour A. A. , Nejati Z.

Biological Letters

|

2013

|

tom 50

|

nr 1

14

Initiation and origin of stigma-like structures [SLS] on ovary and style explants of saffron in tissue culture

75%

Namin M. H. , Ebrahimzadeh H. , Ghareyazie B. , Radjabian T. , Namin H. H.

Acta Biologica Cracoviensia. Series Botanica

|

2010

|

tom 52

|

nr 1

55-60

EN

Saffron, made from the dried stigmas of Crocus sativus L., contains pigments and valuable aromatic compounds, and can be used in medicine and as a spice. Nowadays its production is lower than demand. Tissue culture presents an alternative biochemical tool which can be used to produce stigma-like structure (SLS) in vitro. In this study, the origin and induction of SLS formation was investigated in ovary and style explants of floral buds on MS medium supplemented with 1-naphthalene acetic acid (NAA) and 6-benzlaminopurine (BAP). SLS were directly originated through meristematic cells or indirectly in the form of colorless globular structures from parenchyma tissue. The colorless globular structures initially were conical and pale yellow color at the sharp ends; subsequently they matured into trumpet-like red stigmas with or without finger-like papillae at the margins. Light and electron microscopic observations of ultra- and semithin sections of different developmental stages of SLS showed that these structures possess two kinds of cells: (1) small cells close to parenchyma tissues and (2) large cells oriented towards the peripheral area and apparently originated from the small ones. Our results suggest that the SLS originated from internal parenchyma tissues.

15

Optimizing culture for in vitro pollination and fertilization in cucumis sativus and C. melo

75%

Skalova D. , Navratilova B. , Ondrej V. , Lebeda A.

Acta Biologica Cracoviensia. Series Botanica

|

2010

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tom 52

|

nr 1

111-115

EN

In vitro pollination can be used to overcome crossing barriers in interspecific hybridization within the genus Cucumis. This technique offers a way to produce viable interspecific hybrids. We tested two types of media, designated CP and YS, for in vitro pollination in Cucumis sativus and C. melo. Pollen grains were isolated by centrifugation or directly from mature male flowers and were cultured with mature ovules. We assessed pollen grain viability, fertilization ability, and fertilized ovule development. The developing ovules (becoming enlarged and green) were transferred to media supporting embryogenesis (with ascorbic acid, caseinhydrolysate, coconut water and gibberellic acid). The highest level of regeneration after in vitro pollination was callus formation from ovules. We found caseinhydrolysate to be the most beneficial component during in vitro pollination (CP medium) and during development of fertilized ovules (ON medium). The hybrid character of fertilized ovules arisen from crosses between cucumber and muskmelon was checked but not confirmed by RAPD analysis, for reasons we suggest. The in vitro protocol needs to be optimized further to obtain a high yield of potential hybrid embryos.

16

Cloning, expression and genetic transformation of sucrose phosphate synthase (SPS) gene in Saccharum spontaneum L.

75%

Acta Biologica Cracoviensia. Series Botanica

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2017

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tom 59

|

nr 2

EN

Sucrose phosphate synthase (SPS) is a key enzyme catalyzing sucrose metabolism in plants. In this study, we isolated the SPS cDNA from Saccharum spontaneum and designated as SsSPS (GenBank accession no. MF398541). The full-length of SsSPS cDNA was 4153-bp with an opening reading frame (ORF) of 3132 nucleotides, which encoded a 1043-amino acid protein. The nucleotide sequences alignment showed that it had 98%, 97% and 87% homology with S. officinarum, Setaria italica and Lolium perenne, respectively. Moreover, the SsSPS was detected to express in leaf and stem tissues of S. spontaneum and exhibited a predominant expression in the stem tissue. However, there was no significant difference in the expression level of SsSPS between young leaves and mature ones. Additionally, we generated transgenic S. spontaneum using Agrobacterium-mediated transformation. Our data will provide a valuable foundation for further study of the potential role of SPS in plants.

17

In vitro propagation of Nepalese orchids: a review

75%

da Silva J. A. T. , Acharya K. P.

Journal of Horticultural Research

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2014

|

tom 22

|

nr 2

18

DNA stability contrasts with chromosome variability in Allium fistulosum calli

75%

Mizia P. , Kwolek D. , Ilnicki T.

Acta Biologica Cracoviensia. Series Botanica

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2014

|

tom 56

|

nr 1

19

Production and characterization of tissue cultures of four crocus species from the Carpathian Basin

75%

Acta Biologica Cracoviensia. Series Botanica

|

2017

|

tom 59

|

nr 2

EN

We aimed to produce tissue cultures and plant regeneration from endangered Crocus species: C. scepusiensis, C. tommasinianus, C. vittatus (“Verni” series of the genus) and C. banaticus. For initiation of cultures we used a plant growth regulator (PGR) combination used for in vitro culture of saffron and its relatives: 10 mg L-1 α-naphthaleneacetic acid (NAA) and 1 mg L-1 6-benzyladenine (BA). Shoot tips of young seedlings (C. scepusiensis) and corms (for the rest of species) were used as explants. C. scepusiensis explants developed into organogenic calli. On media with decreased NAA and with or without increased BA concentration, calli produced stigma-like structures and/or shoots and whole plants. In the other species, callus initiation medium induced callus formation with abundant somatic embryos. In C. tommasinianus, embryos developed shoots when auxin content of medium was decreased. In C. banaticus, a decrease of auxin with or without an increase in cytokinin content led to shoot or whole plant regeneration, as in C. scepusiensis. In the case of C. vittatus and C. banaticus, initiation and/or maintenance of cultures on indole-3-butyric acid (IBA) and increased sucrose concentration stimulated whole plant regeneration and in vitro cormlet development. C. scepusiensis and the rest of cultures (organogenic vs. embryogenic) differed at the biochemical level: C. scepusiensis cultures had higher (yet still low) enzymatic antioxidant (catalase, peroxidase) activities. With respect to catalase isoenzyme patterns, C. banaticus was different from the rest of cultures, demonstrating its distinct taxonomical position. Besides germplasm preservation use of the present cultures, they have a potential biotechnological value.

20

The influence of stress conditions on acclimation of magnolia Magnolia x soulangiana Soul.-Bod.

75%

Kamenicka A. , Lanakova M. , Cerna K.

Zeszyty Problemowe Postępów Nauk Rolniczych

|

2002

|

tom 481

|

nr 1

EN

Under a scanning microscope, leaves from tissue cultures (after 8 weeks in vitro culturing), from 1 year old regenerants and from the 100 years old donor tree were studied. More abnormalities were observed in the tissue culture compared to regenerant and donor. During tissue culture transfer rapid, leaf dehydration occurs due to defective regulation mechanisms making stomata of tissue culture stay open. Epidermis as well as stomata cells were observed already on the leaves in in vitro conditions, but they become physiologically functioning only after transfer into conditions of natural environment.

PL

Przy zastosowaniu mikroskopu scaningowego porównywano cechy budowy komórek liści pochodzących z 100 letniej rośliny matecznej, 8 tygodniowych kultur tkankowych oraz 1 rocznych regeneratów. W regenerantach z kultur tkankowych obserwowano więcej nienormalności w budowie komórek epidermy, komórek szparkowych i komórek przyszparkowych w porównaniu z komórkami pochodzącymi z rośliny matecznej. Podczas przenoszenia tkanek w kulturze in vitro obserwowano odwodnienie tkanek liści a mimo to komórki szparkowe pozostawały otwarte. Komórki epidermy oraz komórki szparkowe były obecne na liściach hodowli in vitro jednak stawały się one fizjologicznie funkcjonalne tylko wtedy gdy zostały przeniesione do warunków naturalnych.