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1
Content available remote Cervical Thymic Cyst
100%
|
2007
|
tom 79
|
nr 3
228-231
EN
The Authors of this study present a case of a 13-month old child subjected to surgical intervention for a cervical thymic cyst. The origin of lesions in children is usually associated with remnants from the development of the fetal thymus gland. When the tumor attains significant size, especially in the presence of clinical symptoms, such as respiratory disturbances and problems with feeding, surgical management is required.In spite of the rarity of thymic cysts, it should be considered in cases of differential diagnosis for neck tumors in children. Proper diagnosis is usually possible after the histopathological evaluation of removed tissues. The cervical approach enables removal of the entire lesion, even if partially localized in the thoracic cavity.
EN
Deltamethrin, a well-known type 2 synthetic pyrethroid insecticide, is a widespread environmental toxicant. It has potential to accumulate in body fluids and tissues due to its lipophilic characteristics. The immune system is among the most sensitive targets regarding toxicity of environmental pollutants. Various methods are available in the literature to analyze deltamethrin (DLM) concentration in plasma and tissues, but regarding the immune organs, only one gas chromatography–tandem mass spectrometry (GC–MS/MS) method (on spleen tissues) has been reported. In the present investigation, a rapid and sensitive high-performance liquid chromatography (HPLC) method has been developed and validated to determine DLM concentration in plasma, thymus, and spleen using zaleplone as an internal standard. Liquid chromatography (LC) separation is performed on an Agilent Zorbax® C8 column (250 mm × 4.6 mm, i.d., 5 μm) with isocratic elution using a mobile phase consisting of acetonitrile–5 mM KH2PO4 (70:30, v/v) at a flow rate of 1 mL min−1. The lower limit of quantification (LLOQ) for DLM is 10 ng mL−1 (plasma, thymus, and spleen). The method has been validated in terms of establishing linearity, specificity, sensitivity, recovery, accuracy, and precision (intra- and inter-day) and stabilities study. This validated method was successfully applied to a pharmacokinetic and tissue distribution study of DLM in mice.
EN
Twenty-four male Wistar rats were selected and divided into three groups (control, test group 1, test group 2). The test group 1 was exposed to EMF (50 Hz, 3 mT) 8 h a day, 6 d per week for 2 months. Test group 2 was exposed to EMF (50 Hz, 3 mT) 8 h a day, 6 d per week for 2 months but received orally 30 mg of vitamin E/d. Rats in the control group neither were exposed to electromagnetic field nor received vitamin E. At the end of the experiment, the rats were sacrificed, dissected, and samples from the thymus were taken and processed for light and electron microscopic studies. Forty microscopic fields from each group were randomly selected and studied. The data showed that in the thymus of test group 1, the population of cells in the cortex was decreased but the number of macrophages was increased. EM study showed that cellular nuclei were heterochromatic in comparison to control group. Test group 2 was similar to the control group. These findings indicate that immune system is weakened by electromagnetic field but vitamin E supplementation prevented above alteration.
EN
Localisation of the diaphorase activity of nicotinamide adenine dinucleotide phosphate (NADPH-d), acting as a marker of nitric oxide synthesis (NOS), was studied in the thymus of the rat, mouse and rabbit. The NADPH-d-active cells observed in the rat thymus were irregular in shape with numerous projections and were located on the boundary between the cortex and the medulla. The NADPH-d-active cells in the thymus of the mouse were located predominantly in the medulla. They varied in coloration and their shape was oval, round, or irregular. NADPH-d-positive nerve fibres were located perivascularly. The rabbit thymus displayed a lightly stained cortex, whereas the medulla was seen as a rounded complex of intensively stained cells, without sharp demarcation between them. In the rat thymus, the NADPH-d-positive nerve fibres were not evident, whereas NADPH-d-positive nerve fibres were seen in the perivascular topography of the mouse and the rabbit thymus. These results suggest that NO may participate in the regulation of the thymic function in the species. In summary, the present results reveal the distribution of NADPH-diaphorase-d-positive structures in the rat, mouse and rabbit thymus.
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