Wyniki wyszukiwania - Biblioteka Nauki

1

Wrazliwosc klinicznych szczepow bakterii Gram-dodatnich na wybrane antybiotyki beta-laktamowe

100%

Sawicka-Grzelak A. , Rokosz A.

Medycyna Doświadczalna i Mikrobiologia

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1998

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tom 50

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nr 1-2

69-76

PL

W ciągu 4 miesięcy badań wyhodowano 111 szczepów bakterii Gram-dodat- nich od pacjentów z oddziału chirurgii ogólnej. Wśród wyizolowanych drob¬noustrojów dominowały gronkowce (49 szczepów) i enterokoki (44 szczepy). Wykryto 33 szczepy metycylinoopornych gronkowców. Penicyliny z inhibi¬torami ß-laktamaz oraz karbapenemy wykazały wysoką aktywność in vitro wobec klinicznych szczepów bakterii Gram-dodatnich z wyjątkiem E. fae- cium.

EN

The aim of the study was to determine the activity of four ß-lactam antibiotics against nosocomial strains of Gram-positive bacteria. Two antibiotics combined with ß-lactamase inhibitors: timentin (TIC/CLAV) and tazocin (PIP/TZB) and two carbapenems: imipenem and meropenem were applied. The clinical strains were isolated from patients hospitalized in surgical ward of the National Clinical Hospital No 1 in Warsaw. The strains were identified in the automatic ATB system using ID 32 STAPH, API STREP, API CORYNE and API 20 A strips. The susceptibility of isolates to antibacterial agents was determined in the automatic ATB system using ATB STAPH, ATB STREP and ATB ANA strips. The susceptibility of strains to timentin, tazocin, imipenem and meropenem was tested with disc diffusion method. 111 strains of Gram-positive bacteria were cultured. Staphylococci (49) and enterococci (44) dominated among isolated strains. 33 Staphylococcus spp. strains were identified as methicillin-resistant. The obtained results indicate a significant role of Gram-positive cocci (staphylococci and enterococci) in the aetiology of nosocomial infections. Antibiotics combined with ß-lactamase inhibitors and carbapenems demonstrate broad antibacterial spectrum against clinical strains of Gram-positive bacteria except E. faecium strains.

2

Aktywność przeciwbakteryjna olejku oreganowego (Origanum heracleoticum L.) wobec szczepów klinicznych Escherichia coli i Pseudomonas aeruginosa

86%

Sienkiewicz M. , Wasiela M. , Glowacka A.

Medycyna Doświadczalna i Mikrobiologia

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2012

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tom 64

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nr 4

PL

W niniejszej pracy zbadano aktywność przeciwbakteryjną olejku oreganowego (Origanum heracleoticum L) wobec 2 szczepów wzorcowych, 20 szczepów Escherichia coli oraz 20 szczepów Pseudomonas aeruginosa pochodzących z różnych materiałów klinicznych. Działanie olejku oreganowego oceniano metodą rozcieńczeń w podłożu agarowym, natomiast wrażliwość bakterii na antybiotyki badano metodą dyfuzyjno-krążkową. Na podstawie otrzymanych wyników stwierdzono, że testowany olejek oreganowy był aktywny wobec wszystkich testowanych szczepów klinicznych należących do obu rodzajów bakterii. Jednak szczepy Escherichia coli okazały bardziej wrażliwe. Olejek oreganowy pochodzący z Origanum heracleoticum L posiada właściwości hamujące wobec szczepów klinicznych należących do gatunków Escherichia coli oraz Pseudomonas aeruginosa o różnych wzorach oporności.

EN

Introduction: The aim of this study was to investigate the antibacterial properties of oregano (Origanum heracleoticum L.) essential oil against clinical strains of Escherichia coli and Pseudomonas aeruginosa. The antibacterial activity of oregano essential oil was investigated against 2 tested and 20 clinical bacterial strains of Escherichia coli and 20 clinical strains of Pseudomonas aeruginosa come from patients with different clinical conditions. Methods: The agar dilution method was used for microbial growth inhibition at various concentrations of oil. Susceptibility testing to antibiotics was carried out using disc-diffusion method. Results: The results of experiments showed that the tested oil was active against all of the clinical strains from both genus of bacteria, but strains of Escherichia coli were more sensitive to tested oil. Essential oil from Origanum heracleoticum L. inhibited the growth of Escherichia coli and Pseudomonas aeruginosa clinical strains with different patters of resistance. Conclusions: The obtained outcomes will enable further investigations using oregano essential oil obtained from Origanum heracleoticum L. as alternative antibacterial remedies enhancing healing process in bacterial infections and as an effective means for the prevention of antibiotic-resistant strain development.

3

Wystepowanie beta-laktamaz typu ESBL u paleczek z rodzaju Serratia

86%

Ulatowska B. , Czajkowski H. , Gospodarek H. , Mikucka A.

Medycyna Doświadczalna i Mikrobiologia

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2000

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tom 52

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nr 1

17-24

PL

Enzymy o rozszerzonym spektrum działania (ESBL) wśród klinicznych szczepów pałeczek z rodzaju Serratia wykrywano metodą dwóch krążków (w niewielkiej modyfikacji). Badania wykazały, że 57,7% szczepów dawało reakcję dodatnią.

EN

Serratia spp. has been identified as an important opportunistic pathogen agent in nosocomial infections. The aim of the study was the determination of extended spectrum ß-lactamases (ESBL) occurrence among 78 of Serratia spp. strains isolated in 1996-1998 from clinical specimens obtainted from patients of State Clinical Hospital in Bydgoszcz. Identification of Serratia spp. strains was performed in automatic ATB system with ID 32GN strips (bioMérieux). The strains with ESBL activity were detected by double-disc method according to Jarlier et al. (10) with small modifications. Clavulanic acid, tazobactam and sulbactam were used as the inhibitors of ESBLs. Drug-susceptibility was determinated by disc-diffusion method according to NCCLS standards. Forty-five (57,7%) of the strains were ESBL (+). All of them belonged to S. marcescens species. The majority - 91,1% of strains was derived from urine, 3 from wound and 1 from blood. The obtained results indicate the necessity of monitoring of ESBL-producing strains among gram-negative rods from clinical specimen. The aims of such a procedure are to control and to prevent their dissemination within hospital, as well as to avoid therapeutic failures.