Wyniki wyszukiwania - Biblioteka Nauki

1

Przezywalnosc enterotoksycznych Bacillus cereus w warunkach imitujacych srodowisko zoladka czlowieka

100%

Berthold A. , Ramatowska J.

Medycyna Weterynaryjna

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2008

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tom 64

|

nr 01

101-104

EN

The aim of this work was to determine the survival of spores and vegetative cells of enterotoxic strains of B. cereus in a medium (GM-milk) simulating human stomach environment after consumption of milk or dairy products. Gastric medium (GM-milk) was prepared by mixing equal volumes of a gastric electrolyte solution (4.8 g/l NaCl, 1.56 g/l NaHCO₃, 2.2 g/l KCl, 0.22 g/l CaCl₂, 500 U/l pepsin solution [Sigma-Aldrich Chemie GmbH, nr kat. P6887]) with UHT milk (0,5% fat content). The total count of B. cereus after 6-hours’ incubation in GM-milk (pH~2 and pH~4,5) was determined in a MYP [Merck nr kat. 1.05267.]. After incubating the spores or vegetative cells in a pH~4,5 medium, the count remained unchanged in 70% and 30% strains, decreased by about 1.0 log cfu/ml in 20% and 40% strains or decreased by about 2.0 log cfu/cm³ in 10% and 30% strains, respectively. After incubating the spores in a pH~2 medium, the count remained unchanged in 10% strains, decreased by about 1.0 log cfu/cm³ and 2.0 log cfu/cm3 in 40% and 50% of the strains, respectively. In the same medium no survival of any tested strains of vegetative cells in 1 ml was ascertained after 1 hour incubation. The study demonstrated that the survival of B. cereus depended on the pH of the medium simulating the stomach environment and the physiological state of the cells (spores or vegetative forms).

2

Wplyw adherencji enteropatogennych szczepow Escherichia coli na organizacje cytoszkieletu

84%

Sobieszanska B. M.

Postępy Mikrobiologii

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2002

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tom 41

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nr 2

111-131

3

Test Elisa do wykrywania enterotoksyny LT szczepow Escherichia coli

84%

Osek J.

Medycyna Weterynaryjna

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1996

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tom 52

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nr 11

705-707

EN

The aim of the study was to develop a sensitive and specific ELISA for the detection of LT enterotoxin in E. coli strains. The test was based on the antitoxic antibodies obtained from rabbits immunized with В subunit of cholera toxin (CT). The toxin was structurally closely related with LTB of E. coli. CTB was expressed in a V. cholerae strain harboring a plasmid with a ctxB gene. The toxin was isolated and purified from a growth medium by means of precipitation. A goat antirabbit IgG labeled with HRP antibodies was used as a conjugate. The developed test turned out to be highly specific and sensitive: it was possible to detect from 1.5 to 4.0 ng of LTB. One hundred strains of E. coli isolated from piglets were tested by the LT ELISA. It was found that 67 strains (85.9%) isolated from piglets with diarrhea were LT+ whereas only 2 strains (9.1%) from healthy piglets proved to be enterotoxigenic.

4

Wystepowanie gronkowcow enterotoksycznych w mleku surowym

84%

Bystron J. , Kosek-Paszkowska K. , Molenda J.

Medycyna Weterynaryjna

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2001

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tom 57

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nr 09

645-648

5

Profilaktyka zakazen jelitowych wywolywanych u cielat przez enterotoksyczne szczepy Escherichia coli

84%

Wernicki A.

Medycyna Weterynaryjna

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1996

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tom 52

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nr 10

615-618

6

Biochemiczne wlasciwosci enterotoksycznych i enterokrwotocznych szczepow E. coli

84%

Karakulska J.

Medycyna Doświadczalna i Mikrobiologia

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2002

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tom 54

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nr 3

215-223

PL

Analizowano 30 szczepów E. coli. Typowanie serologiczne wykonano metodą aglutynacji szkiełkowej i przy użyciu testów lateksowych. Na podstawie analizy 35 reakcji metabolicznych, wykazano różnice w aktywności bioche#micznej szczepów wobec 12 substratów. Nie stwierdzono zróżnicowania biochemicznej aktywności szczepów E. coli O157: H7. Wykazano przydat#ność podłoża SMAC oraz podłoży z odczynnikiem MUG do identyfikacji pałeczek okrężnicy należących do tego typu serologicznego.

EN

The purpose of this paper has been to describe the biological characteristics of thirty strains of E. coli. The E. coli strains were isolated from cases of colibacillosis in animals and from human faeces and cow milk samples. Of the thirty analyzed strains, 19 strains (63%) werw found to belong to 7 serogroups: O8, O101, O138, O141, 0147, 0149 and 0157. In 17 strains (57%) fimbriae F4 was discovered and in 1 strain (3%) the presence of fimbriae F5 and F 41 was detected. Serological and biochemical researches, based on the analysis of 35 enzymatic reactions, confirmed that all strains, used in this study, belonged to the species E. coli. The starins demonstrated differences in biochemical activity for 12 substrates. It was found that strains of serotype O157: H7 had biochemical homogeneity, except in their rate of sucrose fermentation and their ability to hydrolyze arginine and sorbitol after longer incubation. On the basis of the biochemical activity, O157: H7 strains were affiliated with biotype C. In identifying serotype O157: H7, SMAC medium with sorbitol and liquid and solid media with MUG reagent were very useful.

7

Enterotoksyny gronkowcowe. Część I. Epidemiologia i znaczenie dla zdrowia publicznego

84%

Korpysa-Dzirba W. , Rola J. G. , Osek J.

Życie Weterynaryjne

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2012

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tom 87

|

nr 08

8

Metody genetyczne i serologiczne w diagnozowaniu entero- i verotoksycznych szczepow Escherichia coli

84%

Nawrotek P. , Karakulska J. , Furowicz A. J.

Folia Universitatis Agriculturae Stetinensis. Zootechnica

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2000

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nr 39

105-112

9

Niektore bakteryjne i wirusowe czynniki etiologiczne biegunek prosiat

67%

Osek J.

Życie Weterynaryjne

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1998

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tom 73

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nr 06

224-227

10

Biegunki cielat wywolane przez E. coli

67%

Bednarski M. , Bednarska M. , Wieliczko A.

Weterynaria w Terenie

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2008

|

tom 02

|

nr 2

42

11

Gronkowce enterotoksyczne w zywnosci

67%

Bystron J. , Molenda J. , Kosek-Paszkowska K.

Życie Weterynaryjne

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2003

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tom 78

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nr 11

650-652

12

Escherichia coli jako czynnik zakazen jelitowych u psow i kotow

67%

Osek J.

Medycyna Weterynaryjna

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2000

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tom 56

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nr 09

547-551

13

Rola szczepów Clostridium perfringens w zakażeniach pokarmowych człowieka

67%

Cichon A. , Wieczorek K. , Osek J.

Medycyna Weterynaryjna

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2012

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tom 68

|

nr 08

EN

Clostridium perfringens is an obligatorily anaerobic, Gram-positive, rod-shaped pathogen, which has the ability to form oval endospores. The microorganism is widespread in environments such as water, soil, waste water, the intestinal tract of humans and animals, as well as in raw and processed food. C. perfringens demonstrates the ability to produce a number of toxins, including enterotoxin (CPE) and major lethal toxins (α, β, ε and ι), which is the basis for a division into five toxinotypes (A, B, C, D, E). It was shown that CPE is encoded by the cpe gene and produced by less than 5% of C. perfringens strains, mainly belonging to type A. Production of enterotoxin is inextricably linked with the process of forming endospores. Considerable evidence implicates CPE as a virulence factor responsible for diarrhea and cramping symptoms associated with C. perfringens type A food poisoning. It is proven that this food poisoning can be caused by strains with the cpe gene located in the bacterial chromosome or on large plasmids, in contrast to other CPE-mediated diseases such as antibiotic (AAD) and sporadic diarrhea (SD), which are associated with plasmid-borne cpe-positive C. perfringens strains. Food poisoning caused by enterotoxigenic strains of C. perfringens type A is one of the most common gastrointestinal diseases in developed countries and shows a positive correlation with the growing percentage of people eating meals in catering establishments.

14

Biegunka poosadzeniowa spowodowana enterotoksyczną E. coli (ETEC)

67%

Luppi A.

Weterynaria w Terenie

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2015

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tom 09

|

nr 3

15

Patogenne Escherichia coli - mechanizmy chorobotwórczości

67%

Poltorak K. , Wieczorek K. , Osek J.

Medycyna Weterynaryjna

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2016

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tom 72

|

nr 06

XX

E. coli are the predominant microorganisms in the human gastrointestinal tract. In most cases, they exist as harmless comensals, and some of them are beneficial to their host in balancing gut flora and absorption of nutrients. However, there are pathogenic strains that cause a broad range of diseases in humans and animals, from diarrhea to bloodstream infections. Among bacterial strains causing these symptoms, seven pathotypes are now recognized: enteropathogenic E. coli (EPEC), shiga toxin-producing E. coli (STEC), enterotoxigenic E. coli (ETEC), enteroinvasive E. coli (EIEC), enteroaggregative E. coli (EAEC), diffusely adherent E. coli (DAEC), and adherent-invasive E. coli (AIEC). Several different strains cause diverse diseases by means of virulence factors that facilitate their interactions with the host, including colonization of the intestinal epithelial surfaces, crossing of the mucosal barriers, invasion of the bloodstream and internal organs or producing toxins that affect various cellular processes. Pathogenic E. coli are commonly studied in humans, animals, food and the environment, in developed and developing countries. The presented paper reviews recent information concerning the pathogenic mechanisms of E. coli, the role of animals and food in the transmission chain and a short overview of epidemiological data.

16

Ustrzec się kolibakteriozy

67%

Kryszewski R.

Hoduj z Głową - Świnie

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2016

|

nr 5

17

Biologiczna aktywność lipopolisacharydów klinicznych szczepów Bacteroides fragilis wyizolowanych w Polsce określona w reakcji z lizatem amebocytów Limulus (LAL)

67%

Rokosz A. , Gorska P. , Michalkiewicz J. , Luczak M.

Medycyna Doświadczalna i Mikrobiologia

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2003

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tom 55

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nr 4

PL

Badaniami objęto lipopolisacharydy wyekstrahowane z dziewięciu klinicznych szczepów B. fragilis wyizolowanych w Polsce. Biologiczną aktywność preparatów LPS oznaczono przy użyciu fotometrycznego testu BET (poprzednio LAL). Aktywność lipopolisacharydów klinicznych szczepów B. fragilis porównano z aktywnością LPS referencyjnych szczepów B. fragilis i LPS E. coli 055:B5. Wśród lipopolisacharydów pałeczek gatunku B. fragilis największą aktywność w reakcji z odczynnikiem LAL wykazał LPS szczepu wyizolowanego z ropnia trzustki.

EN

The aim of this study was to determine a biological activity of lipopolysaccharides (LPS) from clinical Bacteroides fragilis strains isolated in Poland by means of quantitative, photometric BET (LAL) method with Limulus polyphemus amoebocyte lysate and chromogenie substrate S-2423. Lipopolysaccharides were extracted from nine clinical B. fragilis strains by the procedure of Westphal and J arm (1965). Crude LPS preparations were purified with ultracentrifugation. Biological activities of bacterial endotoxins were determined by quantitative BET method with chromogenie substrate S-2423 (ENDOCHROME™ kit). Tests were performed according to the recommendations of the producer (Charles River Endosafe® Ltd., USA). E. coli 055:B5 LPS and LPS preparations from reference B. fragilis strains were applied to compare the results of examinations. Activities of endotoxins from clinical B. fragilis strains isolated in Poland determined in reaction with Limulus amoebocyte lysate were differentiated. Among endotoxins of clinical B. fragilis strains the most active was the preparation from strain cultured in the case of pancreatic ulcer (B. fragilis 80/81 LPS). Lipopolysaccharides of examined B. fragilis strains were less active in BET test than E. coli 055:B5 LPS.

18

Patogeneza zakażeń Escherichia coli u prosiąt

59%

Rzasa A. , Zyzak A. , Urbaniak O.

Przegląd Hodowlany

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2016

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tom 84

|

nr 3

19

Diagnostyka chorob zakaznych przewodu pokarmowego cielat

59%

Bednarski M. , Bednarska M. , Wieliczko A.

Lecznica Dużych Zwierząt. Ogólnopolski Kwartalnik dla Lekarzy Weterynarii

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2010

|

tom 05

|

nr 1

36

20

Escherchia coli i choroba obrzękowa

59%

Wrobel P.

Hoduj z Głową - Świnie

|

2014

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nr 4