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1
Content available Relation of the polymorphism of cyp51A sequence and the susceptibility of Aspergillus fumigatus isolates to triazoles determined by commercial gradient test (Etest) and by reference methods
100%
Nawrot U. , Sulik-Tyszka B. , Kurzyk E. , Mroczyńska M. , Włodarczyk K. , Wróblewska M. , Basak G. , Brillowska-Dąbrowska A.
Acta Biochimica Polonica
|
2017
|
tom 64
|
nr 4
631-634
EN
The aim of this study was to evaluate the accuracy of commercial gradient test (Etest) in the detection of triazole resistant Aspergillus fumigatus isolates using reference microdilution methods and the analysis of sequences of the cyp 51A gene. The study was performed on twenty clinical isolates which were identified as Aspergillus fumigatus based on the DNA sequences of the ITS1-2 fragment of ribosomal DNA and the β-tubulin gene, out of them seventeen isolates showed wild-type cyp51A sequence and three were positive for the mutation TR34/L98H. All isolates were tested for the susceptibility to itraconazole (ITZ), voriconazole (VOR) and posaconasole (POS) using microdilution methods, according to EUCAST and CLSI protocols, as well as using Etest. The results of microdilution and Etests were analysed separately according to clinical breakpoints (CBP) defined by EUCAST version 7.0 and epidemiological cut off values (ECV). Etest as well as reference methods excellently recognised the WT isolates, which were susceptible to all tested triazoles, regardless of the method and CBP or ECV criteria used. The Etest recognized three non-WT isolates as resistant or intermediately sensitive to ITZ and POS and one as resistant to VOR. The categorical concordance between Etests and EUCAST and Etests and the CLSI method ranged from 90 to 100%. The interpretation of the results obtained from routine A. fumigatus Etests requires great caution. The use of the confirmative examinations with reference AST methods as well as with molecular tests is recommended.
2
Susceptibility testing and resistance phenotypes detection in bacterial pathogens using the VITEK 2 system
100%
Stefaniuk E. , Mrowka A. , Hryniewicz W.
Polish Journal of Microbiology
|
2005
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tom 54
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nr 4
EN
A set of well characterized strains, collected in Polish hospitals, including Gram-negative (n = 93) and Gram-positive (n = 90) isolates was used in the study. The VITEK 2 AST-cards were used in the analysis according to the manufacturer's recommendations. Comparison of the susceptibility data obtained by the standard method and by VITEK 2 cards proved concordant in 99% of cases. Clinically important mechanisms were revealed by the VITEK 2 AES with > 95% agreement with reference data including methicillin resistance in staphylococci (98%), high-level aminoglycoside resistance in enterococci (100%), VanA and VanB phenotypes in enterococci (100%), and ESBLs in Enterobacteriaccae (93.8%). The VITEK 2 AES System appears a reliable tool for the detection and interpretive reading of clinically important mechanisms of resistance and can be recommended for routine work.
3
Content available Identification modeling of Polish electric power exchange
86%
Marlęga R. , Tchórzewski J.
Information Systems in Management
|
2016
|
tom Vol. 5, No. 2
195--204
EN
This paper contains selected results of identification modeling of Polish Electric Power Exchange (EPE). In order to obtain EPE system model it was performed identification based on figures of EPEís Day-Ahead Market. During performing identification process, parametric arx model in System Identification Toolbox environment was utilized. Generated EPE parametric model has been further used for performing simulation tests and realization of susceptibility testing. Suitable models were implemented in Simulink software. As a result of simulation and susceptibility testing, many interesting findings has been delivered.
4
Istotne mechanizmy oporności pałeczek Gram-ujemnych na antybiotyki β-laktamowe
72%
Żabicka D.
Laboratorium - Przegląd Ogólnopolski
|
2015
|
tom nr 7-8
47--50
PL
U pałeczek Gram-ujemnych do najważniejszych mechanizmów nabytej oporności na antybiotyki β-laktamowe należą zdolność do produkcji β-laktamaz o rozszerzonym spektrum substratowym (ESBL) oraz karbapenemaz KPC, MBL i OXA-48. W opracowaniu omówiono wymienione mechanizmy i wskazania do ich wykrywania. Przedstawiono również metody wykrywania produkcji tych enzymów stosowane w rutynowych oznaczeniach w laboratoriach mikrobiologicznych.
EN
In Gram-negative bacteria the most important mechanisms of acquired resistance to β-lactam antibiotics are the production of extended-spectrum β-lactamases (ESBL) and carbapenemases KPC, MBL and OXA-48. Those mechanism of resistance, the indication for testing as well as the methods used in the routine microbiology laboratory for their detection were described in this paper.
5
Detekcja i identyfikacja drobnoustrojów - oznaczanie lekowrażliwości bakterii
72%
Żabicka D.
Laboratorium - Przegląd Ogólnopolski
|
2012
|
tom nr 7-8
41--45
PL
W medycznych laboratoriach mikrobiologicznych do oznaczania lekowrażliwości bakterii stosowane są systemy automatyczne oraz metody manualne, takie jak metoda dyfuzyjno-krążkowa i oznaczanie minimalnego stężenia hamującego MIC metodą dyfuzji z paska z gradientem antybiotyku. Interpretacja wyników oznaczania lekowrażliwości jest dokonywana z zastosowaniem wartości granicznych EUCAST.
EN
Antimicrobial susceptibility testing of bacteria isolated in the medical microbiology laboratories is performed with using automated systems as well as manual methods, such as disc diffusion method and gradient strip minimal inhibitory concentration (MIC) evaluation. EUCAST breakpoints are used for interpretation of susceptibility testing results.
6
Content available Indices and their applicability under high temperature in potato (Solanum tuberosum L.) cultivars
44%
Paul S. , Gogoi N.
International Letters of Natural Sciences
|
2015
|
tom 43
EN
The increasing temperature is going to be more vulnerable for cool season crops like potato which requires an optimum productivity temperature of 18 to 20 °C. Thus, breeding for heat tolerance has become very important. Therefore, some previously used indices for abiotic stress tolerance have been used in our study for screening of high temperature stress tolerance in potato. Three high yielding (Kufri jyoti, Kufri megha and Kufri pokraj) and two local (Rangpuria and Badami) commonly grown potato cultivars were selected for our experiment. Potato cultivars were sown under normal condition and two high temperature conditions (polyhouse and early season) and indices such as HSI (heat susceptibility index), HTI (heat tolerance index), GM (geometric mean) and HII (heat intensity index) were used to evaluate the performance of the cultivars under all the three temperature conditions. The positive and significant correlation between HTI (heat tolerance index), and GM (geometric mean) as well as with tuber yield under all the conditions revealed that these indices were efficient in selecting the high temperature tolerant potato cultivars. We recorded the equal applicability of these two indices for both high yielding and local group of potato cultivars. Our study revealed that cultivar Kufri megha and Rangpuria showed higher heat tolerance between high yielding and local cultivars respectively.
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