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nr 3
695-710
EN
In this paper the problem of accurate edge detection in images of heat-emitting specimens of metals is discussed. The images are provided by the computerized system for high temperature measurements of surface properties of metals and alloys. Subpixel edge detection is applied in the system considered in order to improve the accuracy of surface tension determination. A reconstructive method for subpixel edge detection is introduced. The method uses a Gaussian function in order to reconstruct the gradient function in the neighborhood of a coarse edge and to determine its subpixel position. Results of applying the proposed method in the measurement system considered are presented and compared with those obtained using different methods for subpixel edge detection.
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tom 80
EN
The degree of dependence of a lipid bilayer’s surface properties on its conformational state is still an unresolved question. Surface properties are functions of molecular organization in the complex interfacial region. In the past, they were frequently measured using fluorescence spectroscopy. Since a fluorescent probe provides information on its local environment, there is a need to estimate the effect caused by the probe itself. In this paper, we address this question by calculating how lipid head-group orientation effects the fluorescence intensity of Fluorescein-PE (a probe that is sensitive to surface potential). In the theoretical model assumed the lipid bilayer state and the interactions between the charged fluorescent probe and the surrounding lipid molecules was evaluated. The results of this theoretical analysis were compared with experimentally obtained data. A lipid bilayer formed from DPPC was chosen as the experimental system, since it exhibits all the major conformational states within a narrow temperature range of 30°C - 45°C. Fluorescein-PE fluorescence intensity depends on local pH, which in turn is sensitive to local electrostatic potential in the probe’s vicinity. This local electrostatic potential is generated by lipid head-group dipole orientation. We have shown that the effect of the probe on lipid bilayer properties is limited when the lipid bilayer is in the gel phase, whereas it is more pronounced when the membrane is liquid-crystalline. This implies that Fluorescein-PE is a good reporter of local electrostatic fields when the lipid bilayer is in the gel phase, and is a poor reporter when the membrane is in the liquid-crystalline state.
EN
Rye plants were grown in a nutrient solution prepa- red according to Hoagland for 2 weeks at pH 7, next for 14 days at pH 4.5 (without Cu+2) and in the presence of 20, 50, or 100 mg dm-3 copper ions. The control plants were grown continuously at pH 7. The physicochemical surface properties of the roots were exa- mined using two adsorbates – polar (water vapour) and non-polar (nitrogen). The surface properties of the roots grown at pH 4.5 without Cu+2 were apparently the same as those of controls. The roots of rye which grew in the presence of Cu+2 were characte- rized by lower (relative to controls) specific surface area values. Statistically significant differences in the size of the apparent surface area (determined by water vapour) were reported for roots in- cubated with copper ions at a concentration of 20 and 50 mg dm-3. The average water vapour adsorption energy of the root surface decreased under the stress conditions. There were no statistically significant differences for the free surface area and characteristic energy of nitrogen adsorption.
EN
In this paper the problem of accurate edge detection in images of heat-emitting specimens of metals is discussed. The images are provided by the computerized system for high temperature measurements of surface properties of metals and alloys. Subpixel edge detection is applied in the system considered in order to improve the accuracy of surface tension determination. A reconstructive method for subpixel edge detection is introduced. The method uses a Gaussian function in order to reconstruct the gradient function in the neighborhood of a coarse edge and to determine its subpixel position. Results of applying the proposed method in the measurement system considered are presented and compared with those obtained using different methods for subpixel edge detection.
12
Content available A method of improving the surface of HDF
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