The method does not differ in difficulty from the standard surface temperature imaging method, which is its unquestionable advantage. The disadvantage is the need to use test models made of materials transmitting visible light in the full range (blue light for excitation and red as a recorded result), which, with more complex shapes, makes the model more expensive. Nevertheless, the presented method is a valuable supplement to the set of methods and techniques used so far for imaging temperature fields and estimating the heat flux in the steady state.
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Microscopic methods usable for sample surface imaging and subsequent qualitative and quantitative evaluation of platelet adhesion to the surface of the biomaterial studied were compared. It was shown, making use of the samples of medical steel (AISI 316L), that such tools as surface imaging with scanning electron microscopy (SEM), glutaraldehyde induced fluorescence technique (GIFT) and metallurgical microscopy (MM) are equivalent in evaluating surface platelet adhesion. The importance of biological variability of blood samples for a proper result assessment and the necessity of using internal standards were also considered.
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