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The role of sulfation in the processing of mucus glycoprotein in gastric mucosa was investigated. Rat gastric mucosal segments were incubated in MEM at various medium sulfate concentrations in the presence of [³⁵S]Na₂ SO₄ [³H] glucosamine and [³H]proline, with and without chlorate an inhibitor of PAPS formation. The results revealed that the mucin sulfation attained maximum at 300 µM medium sulfate concentration. Introduction of chlorate into the incubation medium, while having no effect on the protein synthesis as evidenced by [³H]proline incorporation, caused at its optimal concentration of 2 mM a 90% decrease in mucin sulfation and a 40% drop in mucin glycosylation. Evaluation of mucin molecular forms distribution indicated the predominance of the high molecular mucin form in the interacellular fraction and the low molecular mucin from in the extracellular fraction. Increase in medium sulfate caused an increase in the high molecular weight mucin form in both fractions, and this effect was inhibited by chlorate. Also, higher medium sulfate concentrations led to a higher degree of sulfation in the high molecular weight mucin form, the effect of which was inhibited by chlorate. The results suggest that the sulfation process is an early event taking place at the stage of mucin subunit assembly and is required for mucin polymer formation. Hence, the disturbances in mucin sulfation process could be determinal to the maintenance of gastric mucus coat integrity.
EN
In this work extraction of copper and iron from a reverberatory furnace slag was studied. A two-step extraction procedure was followed. The first step was roasting of the slag in the presence of sulphuric acid at temperatures between 150 and 800oC. The second step was leaching of the resulting calcine with distilled water. The maximum copper extraction of about 94% was achieved. In this case, the slag was roasted at 250oC with sulphuric acid higher of about 33% than that stoichiometrically required, followed by water leaching of calcine at 50oC. About 55% of iron was also dissolved under these conditions. On the other hand, for the calcine obtained at sulphation temperature of 600oC, extraction of copper in a water leaching stage was still relatively high (about 79%), whereas that of iron was comparatively low (about 6%). Dissolution of copper and iron from the calcine was found to be very fast and was complete within the first few minutes. The water temperature in the leaching step was found to have no effect on extraction of copper and iron from the calcine in the range of 30 to 85oC.
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