Wyniki wyszukiwania - Biblioteka Nauki

1

Effect of sucrose concentration on oxalic acid biosynthesis by Aspergillus niger

100%

Walaszczyk E. , Gasiorek E. , Podgorski W.

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tom 588

EN

The aim of the work was to determine the influence of initial sucrose concentration in fermentation medium on oxalic acid synthesis by Aspergillus niger W78C in submerged batch culture. Cultivations were conducted in synthetic medium containing sucrose in the concentration of 100, 125, 150, 175 or 200 g·dm⁻³. As the result of the present investigation, the optimal sucrose concentration was found at the level of 125 g·dm⁻³. In medium containing this amount of carbon source, 70.2 g·dm⁻³ of oxalic acid was obtained. When substrate concentration was the highest, 200 g dm⁻³, the amount of product was only 5.2 g·dm⁻³ higher than in medium with 125 g·dm⁻³ of sucrose. Moreover, an increase of the initial sucrose concentration resulted in process time extension, an increase of citric and gluconic acids concentration and a decrease of productivity and oxalic acid yield.

PL

Celem badań było określenie wpływu początkowego stężenia sacharozy, będącej jedynym źródłem węgla, na proces biosyntezy kwasu szczawiowego przez Aspergillus niger metodą wgłębnej hodowli okresowej. W badaniach stosowano szczep A. niger W78C. Hodowle prowadzono w podłożach syntetycznych zawierających sacharozę w stężeniu 100, 125, 150, 175 lub 200 g·dm⁻³. W podłożu o najniższym początkowym stężeniu sacharozy, tj. 100 g·dm⁻³, uzyskano najniższe stężenie kwasu szczawiowego, tj. 59,3 g·dm⁻³. Podwyższenie stężenia substratu do 125 g·dm⁻³ spowodowało zwiększenie ilości produktu do 70,8 g·dm⁻³, czyli o 19,1%. Dalszy wzrost stężenia cukru w podłożu nie był już tak skuteczny, gdyż w wariancie z najwyższym początkowym stężeniem sacharozy, tj. 200 g·dm⁻³, uzyskano tylko o 5,2 g·dm⁻³ (czyli o 7,4%) więcej kwasu szczawiowego niż w podłożu, w którym ilość substratu była równa 125 g·dm⁻³. Wzrost początkowego stężenia substratu ze 100 do 200 g·dm⁻³ powodował wydłużenie czasu procesu, wzrost stężenia kwasów towarzyszących: cytrynowego i glukonowego, oraz spadek wydajności kwasu szczawiowego i szybkości jego tworzenia. Na podstawie uzyskanych stężeń produktu w podłożach z różnym początkowym stężeniem substratu oraz po analizie parametrów procesu (wydajności kwasu szczawiowego i objętościowej szybkości jego produkcji) uznano, że najkorzystniejsze stężenie sacharozy w tym procesie wynosi 125 g·dm⁻³.

2

The influence of sucrose concentration on microtuber formation of Gloriosa rothschildiana O'Brien

100%

Kozak D.

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tom 08

3

Differential adaptation of membranes of two osmotolerant fungi, Aspergillus chevalieri and Penicillium expansum to high sucrose concentrations

100%

Hefnawy M. A. , Abou-Zeid A. M.

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nr 1

4

The influence of various sucrose concentrations, pH and hormone inhibitors on the efficiency of somatic embryogenesis in two fern species: Cyathea delgadii and Asplenium cuneifolium

86%

Grzyb M. , Tomiczak K. , Rybczynski J. J. , Mikula A.

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2015

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tom 96

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nr 1

5

Sucrose and cytokinin interactions in relation to ethylene and abscisic acid production in the regulation of morphogenesis in pelargonium x hortorum L.H. Bailey in vitro

86%

Wojtania A. , Wegrzynowicz-Lesiak E. , Dziurka M. , Waligorski P.

Acta Biologica Cracoviensia. Series Botanica

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2015

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tom 57

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nr 1

EN

The aim of the study was to determine the effect of exogenous sucrose and cytokinin on ethylene production and responsiveness in relation to the shoot formation of Pelargonium × hortorum 'Bergpalais' in vitro. Increasing the concentration of sucrose from 15 to 40 g L-1 in medium containing meta-topolin (mT) resulted in a two-fold decrease in the number of shoots and leaves as well as a reduction in ethylene production. The addition of ethylene synthesis inhibitor (AVG) to mT-medium significantly reduced the ethylene production and the shoot growth, but it had no significant influence on the shoot formation. The mT-induced shoot formation was, however, significantly reduced in the presence of ethylene action inhibitor (AgNO3), in a manner dependent on sucrose levels. At the end of the subculture period, increased sucrose concentrations (15–40 g L-1) in the presence of mT and AgNO3 resulted in a 3.7-fold increase in ethylene emission. At the same time, the supply of sucrose caused a 2.8-fold increase in the level of endogenous abscisic acid (ABA). Our results may suggest that the inhibitory effect of high sucrose concentration (30 and 40 g L-1) may depend on its influence on ethylene sensitivity. It also suggests that sucrose-regulation of the shoot formation of Pelargonium in vitro is mediated by ABA.

6

Effect of sucrose concentration on regeneration in vitro from different explants of Fritillaria imperialis L.

86%

Witomska M. , Lukaszewska A.

Annals of Warsaw Agricultural University. Horticulture

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2000

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tom 21

7

Effect of sucrose concentration BA and NAA on regeneration in vitro of Hippeastrum x chmielii Chm.

72%

Witomska M.

Annals of Warsaw Agricultural University. Horticulture (Landscape Architecture)

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2002

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tom 23

8

Influence of type and concentration of sugars, supplemented with 8-hydroxyquinoline sulphate, on the vase life of waxflower

58%

Dung C. D. , Seaton K. , Singh Z.

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2017

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tom 29

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nr 1

9

Effect of medium type, benzyladenine and sucrose on micropropagation of Hippeastrum x chmielii Chm.

58%

Witomska M. , Lukaszewska A. , Wojtowicz M.

Annals of Warsaw University of Life Sciences - SGGW. Horticulture and Landscape Architecture

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2007

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tom 28

10

Effect of various preculture and osmotic dehydration conditions on cryopreservation efficiency and morphogenetic response of chrysanthemum shoot tips

51%

Kulus D. , Serocka M. , Mikula A.

Acta Scientiarum Polonorum. Hortorum Cultus

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2018

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tom 17

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nr 1

EN

The aim of this study was to evaluate the influence of sucrose concentration in the preculture medium, as well as the duration of osmotic dehydration on the efficiency of chrysanthemum ‘Richmond’, ‘Lady Orange’ and ‘Lady Salmon’ cryopreservation by encapsulation-dehydration technique. For all cultivars tested, the best regrowth of cryopreserved shot tips was recorded with 0.25 M sucrose concentration and 10 µM ABA during a two-week preculture, followed by a 4- or 7-day osmotic dehydration. The survival rate ranged from 56.8–58.0% (the Lady group) to 63.6% (‘Richmond’). However, the ability to grow was smaller and reached 18.2–50.7%. It was found that higher sucrose concentration during the preculture slowed the growth of chrysanthemum shoot tips and led to an increased formation of multiple shoots (by activating axillary buds) or deformed adventitious shoots (incapable of further growth). The frequency of tissue hyperhydricity also increased, while the rhizogenesis efficiency decreased when higher sucrose concentration in the preculture medium was applied. The influence of osmotic dehydration duration on the explants morphogenetic response was cultivar-dependent.