Wyniki wyszukiwania - Biblioteka Nauki

1

Histoenzymological localization of glycerol 3 phosphate dehydrogenase and succinate dehydrogenase in the mesencephalon of a minor carp Barilius bendelisis (Hamilton)

100%

Singh N.

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tom 18

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nr 2

2

Morphohistochemical profile of red and pink muscles in freshwater fish

84%

Nejedli S. , Kozaric Z. , Gjurcevic Kantura V. , Zobundzija M. , Petrinec Z. , Matasin Z. , Vlahovic K.

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nr 11

1307-1310

EN

The morphohistochemical profile of red and pink muscles from the caudal portion of the trunk at the height of the horizontal septum in the Carassius auratus gibelio and Perca fluviatilis 20-25 cm long from the Gare.nica (Croatia) fish farm have been studied. Muscle samples, taken on arrival from live fish, were frozen in liquid nitrogen, cut on the cryo cut into 10 µm thick slices stained with hemalum and eosin and with the procedure for the detection of succinate dehydrogenase (SDH). The diameter of muscle fibers from the obtained preparations was measured using a micrometer scale with 10 µm divisions on a Nikon microscope. The red muscle of the Carassius auratus gibelio and Perca fluviatilis is known to have a triangular cut. It is positioned laterally beneath the skin and runs craniocaudally along the horizontal septum. Underneath the surface red muscle there is a pink muscle, shorter than the red one. In the Carassius auratus gibelio it is wide and divided into the surface (pink I) and deep (pink II) layer, while in Perca fluviatilis it is narrow, short and unchanged, but comprises a narrow transitional zone towards the red muscle. The red muscle is composed of red muscle fibers of small diameter with potent SDH activity. The pink muscle is composed of two types of fibers . intermediary small-diameter muscles of pronounced SDH activity and large-diameter muscles of less pronounced SDH activity as well as white muscles of large diameter with poor SDH activity. Intermediary fibers in the Carassius auratus gibelio accounted for 77.80% of the fibers in the pink I layer and 44.90% of the fibers in the pink II layer. White fibers in the same fish accounted for 22.20% of the fibers in the pink I layer and 55.10% of the fibers in the pink II layer. Intermediary fibers in the pink muscle of the Perca fluviatilis accounted for 58.80% fibers, whereas white fibers accounted for 41.20% of all fibers.

3

Fluorek - kompetycyjny i niekompetycyjny inhibitor dehydrogenazy bursztynianowej

84%

Stachowska E. , Chlubek D. , Machoy Z.

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tom 32

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nr 2

111-117

PL

Zbadano typ hamowania dehydrogenazy bursztynianowej [E.C. 1.3.99.1] (SDH) przez fluorek w zmiennych warunkach doświadczenia. Badania prowadzono z preparatami cząstek submitochondrialnych, co pozwoliło śledzić zachowanie enzymu w naturalnym otoczeniu błony mitochondrialnej. Ustalono, że w zależności od warunków doświadczalnych - a więc Stężeniu fluorku i bursztynianiu w roztworze, jon fluorkowy może zachowywać się jak inhibitor kompetycyjny lub niekompetycyjny.

EN

Effect of fluoride ions on the activity of succinate dehydrogenase was studied for different fluoride and substrate concentration: 0,025 mol/dm3, 0,50 mol/dm3, 0,75 mol/dm3, 0,1 mol/dm3, using Lineweaver-Burk and Dixon's methods. As it is evident from the enclosed diagrame the inhibition of succinate dehydrogenase induced by the presence of fluoride is not the inhibition being of exclusively competitive character. Resorting to additional measurement and calculation method it was revealed that fluoride - induced SDH inhibition was of mixed - competitive and noncompetitive character.

4

The influence of heavy metal ions on the viability and metabolic enzyme activity of the marbled crayfish Procambarus virginalis (Lyko, 2017)

67%

Marenkov O. , Prychepa M. , Kovalchuk J.

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tom 70

EN

The article shows the results of studies on the influence of heavy metal ions (manganese, nickel, lead) on the viability and metabolic enzyme activity of marbled crayfish Procambarus virginalis (Lyko, 2017) (Decapoda). Due to the fact that marbled crayfish got into the reservoirs of the Dnipropetrovsk region in 2015, it was necessary to study the possibilities of its adaptation to environmental factors of reservoirs for further prediction of its distribution or even acclimatization under conditions of toxicological contamination of the ponds of the steppe Prydniprovya. In the experiment with marbled crayfish, chronic effects of various concentrations of heavy metal ions on the physiological state and enzyme activity were investigated. The obtained results showed that among the investigated heavy metals nickel ions influenced the weight indexes and mortality of crustaceans the most negatively. According to the results of the research, significant changes were noted in the individual biochemical parameters of marbled crayfish under the influence of manganese, lead and nickel ions. The most significant changes in the activity of lactate dehydrogenase were detected in muscle tissues affected by manganese and nickel ions. A significant decrease in the activity of succinate dehydrogenase in muscle of marbled crayfish was determined after the action of heavy metal ions. Investigation of changes in the activity of alkaline phosphatase under the influence of the ions of manganese, lead and nickel has its own characteristics, which indicates certain violations in the tissues of cell membranes. Changes in the activity of enzymes were also reflected in the overall protein content. In conclusion, changes in these parameters may indicate a rapid biochemical response of crustaceans to the toxic effects of heavy metals.

5

Jon fosforanowy kompetycyjnym inhibitorem dehydrogenazy bursztynianowej

67%

Stachowska E. , Machoy Z. , Chlubek D.

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nr 3

263-267

PL

Badano zależność pomiędzy aktywnością dehydrogenazy bursztynianowej [E.C. 1.3.99.1] a jonem fosforanowym w niejonowym roztworze buforu Tris-kakodylowego. Preparatem pomiarowym były cząstki submitochondrialne, pozbawione szczawiooctanu - inhibitora związanego z centrum aktywnym enzymu. Na podstawie przeprowadzonych badań wykazano, że fosforan jest inhibitorem kompetycyjnym enzymu (przy stosunkowo niskich jak dla warunków pomiarowych stężeniach fosforanu - rzędu 10 mmoli/dm3). Uzyskane wyniki zwracają uwagę na fakt, że stosowane często do pomiaru aktywności enzymów bufory fosforanowe (gdzie stężenie fosforanu osiąga nawet 200 mmoli/dm3) mogą wpływać niekorzystnie na aktywność niektórych enzymów oddechowych.

EN

Effect of phosphate ions at various concentrations on succinate dehydrogenase activity was studied by the Lineweaver-Burk method. It has been found that the phosphate ion acts as a competitive inhibitor of the enzyme.

6

Histochemical studies on Fasciola hepatica in mice. II. Oxidoreductase activity in experimentally induced fasciolosis

67%

Kolodziejczyk L.

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nr 4

EN

CFW strain white mice were infected with various doses (10, 30, 50 and 100) of Fasciola hepatica metacercariae administered per os. Using histochemical methods, the activity of selected oxidoreductases (α-glycerophosphate dehydrogenase, isocitrate dehydrogenase, succinate dehydrogenase, and cytochrome oxidase) was evaluated in the liver of the mice suffering from acute and chronic fasciolosis. Intensified anaerobic and aerobic respiration was recorded. The host’s compensatory mechanism observed in the form of intensified metabolism, was found to be related to the infection intensity.

7

Oddzialywanie kadmu w zaleznosci od dawki i stopnia kumulacji na jelito cienkie ciezarnych samic szczurzych

51%

Kaminska O.

Bromatologia i Chemia Toksykologiczna

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1995

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tom 28

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nr 2

161-167

PL

Intoksykacja kadmem ciężarnych samic szczurzych od 7 do 19 dnia ciąży, w dawkach w zależności od grupy doświadczalnej równych 0,2, 2,0 oraz 20,0 mg Cd/kg masy ciała, powodowała w jelicie cienkim spadek aktywności procesów fosforylacji oksydatywnej korelujący z wielkością dawki oraz zawartością metalu w ścianie badanych odcinków jelita cienkiego. Równolegle obserwowano aktywację beztlenowych szlaków uzyskiwania energii.

EN

This work constitutes the continuation of a larger series intended to explain the bacground of prenatal cadmium sensitivity of pregnant female rats by analysing the relationship between the functional and structural condition of pregnant female rat organs and the dose and degree of cadmium accumulation. 60 pregnant female rats were used in the experiment. Between 7 and 19 day of gestation they were given cadmium by means of stomach tube at daily doses of 0,2,2,0 and 20,0 mg Cd/kg body weight, depending on the group to which they were assigned. Dose-dependent reduction of oxidative phosphorylation activity was observed whithin duodenum and jejunum. The reduction resulted in energy deficit, which triggered the anaerobic processes of energy production. Cadmium intoxication also resulted in statistically significant increase of cadmium concentration in the walls of the examined portions of the small intestine of all experimental groups. Cadmium accumulation was higher in the duodenum than in the jejunum.