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The usefulness of RAPD and AFLP markers for determining genetic similarity in rye [Secale L.] species and subspecies

100%

Cwiklinska A. , Broda Z. , Bocianowski J. , Dobrzycka A.

Acta Biologica Cracoviensia. Series Botanica

2010

tom 52

nr 1

19-25

In this work we searched for genetic similarities in twelve wild rye species and subspecies and a control (S. cereale ssp. cereale, cv. Walet), using RAPD and AFLP markers. AFLP is useful for distinguishing homo- and heterozygotes but is not recommended for evaluation of codominant markers. We assessed the usefulness of the applied methods for examining genetic similarity in rye. RAPD yielded four groups of genetic similarity, with similarity values between 0.32 and 0.81. AFLP markers distinguished two groups of genetic similarity, ranging from 0.49 to 0.79.

Intraspecific molecular variation of Allium ursinum (Amaryllidaceae) across the border of two subspecies distribution ranges

84%

Rola K. , Lenart-Boron A. , Boron P. , Osyczka P.

Acta Biologica Cracoviensia. Series Botanica

2015

tom 57

nr 1

The study investigates the genetic differentiation among two subspecies of Allium ursinum L., namely A. ursinum subsp. ursinum and subsp. ucrainicum as well as their putative hybrid that is represented by individuals with intermediate morphology. Inter-Simple Sequence Repeats (ISSR) were applied to determine the status of intermediate morphotypes in terms of their genetic pattern and to assess the level of genetic variability within and between various populations of A. ursinum. The study comprises 144 specimens from nine populations along the east-west transect in Poland, which includes localities of both subspecies and their putative hybrid. Among the examined populations, 48 bands were amplified, of which 45 were found to be polymorphic. The principal coordinate analysis (PCoA), the neighbour-net analysis and Mantel test showed a strong correlation between genetic variability and geographic distance. Analysis of molecular variance (AMOVA) revealed that a greater proportion of total genetic variation resided within populations rather than among them. The Structure Bayesian clustering analysis revealed the presence of three distinct genetic groups within studied populations, where 'eastern' genotypes correspond to A. ursinum subsp. ucrainicum, and 'western' to subsp. ursinum; whereas the third genetic group has the largest share in the individuals occurring at the border of the distribution ranges of both subspecies. The emergence of the third genetic group is probably an effect of hybridization events occurring within the secondary contact zone. Typical morphologically intermediate populations occur only in a relatively narrow geographical zone, but the hybrid zone revealed by molecular markers is actually much wider than it is suggested by the morphological pattern of individuals. The current distribution pattern of both subspecies of A. ursinum and their hybrid zone is related to the two main directions of postglacial migration of Fagus sylvatica to the area of Poland. The hybrid zone arose as an effect of the secondary contact of two divergent lineages of A. ursinum.

Gymnadenia conopsea (L.) R. BR. subsp. densiflora (Wahlenberf) Richter - nowy takson Tatrzańskiego Parku Narodowego i flory Polski

67%

Bernacki L.

tom 19

Blaisdell's formae and homonyms in the genus Eleodes Eschscholtz [Coleoptera: Tenebrionidae: Embaphionini]

67%

Thomas D. B.

Annales Zoologici

2005

tom 55

nr 4

The taxonomy of the speciose genus Eleodes Eschscholtz is complicated by a plethora of infrasubspecific names proposed by Blaisdell which were subsequently, if dubiously, elevated to subspecies. These cases are discussed and where deemed necessary, resolved. The following new names are proposed to replace occupied names: Eleodes formosus for E. oblongus Blaisdell, Eleodes tribulus for E. caudatus (Horn), Eleodes extricatus convexinotus for E. e. convexicollis Blaisdell, and Eleodes transvolcanensis for E. alticolus Pierre. Eleodes transvolcanensis rufipes Pierre is a new trinomial combination resulting from the new name for E. alticolus. Eleodes lecontei Horn is conserved by precedence over E. lecontei Gemminger et Harold.

Rody koni arabskich

59%

Upton P.

Araby

2011

tom 06

nr 3(22)