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nr 03
113-114
EN
The studies were done on 50 male Wistar rats divided into five groups: 1 - controls, II - rats immobilized for 13 hr, III - animals immobilized after peritoneal infection with a pathogenic E. coli, IV - rats immobilized after the application of vitamin E (30 mg/kg bw) for six continous days, V - rats immobilized after the application of vitamin E and peritoneal injection of a pathogenic E. coli. Metabolic neutrophil activity (NBT-test) was determined spectrophotometrically in blood and the level of corticosterone was estimated in blood plasma by the radiocompetition method. It was found that an increase of corticosterone and decrease of metabolic neutrophil activity appeared in rats after immobilization (group II). However, a significant decrease of neutrophil activity and an inconsiderable increase of corticosterone were noted in group III and V. Immobilization of rats after the administration of witamin E did not affect the level of the examined parameters (group IV).
EN
The level of adrenaline, noradrenaline and corticosterone in blood plasma of adult chickens immobilized by fastening of legs and wings was examined after 1, 3, 6, 12 and 24 h since the beginning of immobilization. The level of adrenaline and corticosterone increased significantly after 3 and 6 h since the beginning of immobilization and then steadily decreased. The concentration of noradrenaline significantly lowered during the whole period of immobilization. It was also noted a slight increase of metabolic activity of blood neutrophils measured in the NBT. These results point to decrease of the suprarenal glands activity (adaptation) just after a few hours of stress.
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